Notch Signaling as Gatekeeper of Rat Acinar-to-β-Cell Conversion in Vitro

Baeyens, Luc; Bonné, Stefan; Bos, Tomas; Rooman, Ilse; Peleman, Cindy; Lahoutte, Tony; German, Michael S.; Heimberg, Harry; Bouwens, Luc

doi:10.1053/j.gastro.2009.01.047

Cited by 74 publications

(72 citation statements)

References 45 publications

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“…Several reports suggested that activation of EGF signaling induces transdifferentiation of pancreatic exocrine cells into endocrine cells (5,20). To understand the functional consequences of ligand-bound TR␣ with downstream signaling molecules of the EGF receptor, we focused on PI3K.…”

Section: Resultsmentioning

confidence: 99%

“…Because of the higher expression of this sugar on exocrine cells, WGA labels only the acinar cells (20). Real-time RT-PCR-RNA was extracted from tissues or cells by using an RNeasy mini kit (Qiagen) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Reprogramming of Acinar Cells to Insulin-producing Cells-The lectin WGA binds to N-acetylglucosamine and remains in the cytoplasm of pancreatic acinar cells (20). To visualize acinar cells, the purified exocrine fraction was incubated with fluorescein-conjugated lectin and infected with adenovirus.…”

Section: Ligand-bound Tr␣ Enhances the Expression Of Transcription Famentioning

confidence: 99%

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Ligand-bound Thyroid Hormone Receptor Contributes to Reprogramming of Pancreatic Acinar Cells into Insulin-producing Cells

Furuya¹,

Shimura

Asami³

et al. 2013

Journal of Biological Chemistry

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Background: One goal of diabetic regenerative medicine is to convert mature pancreatic acinar cells into insulin-producing cells. Results: Ligand-bound thyroid hormone receptor ␣ (TR␣), which interacts with p85␣, induces phosphatidylinositol 3-kinase (PI3K) signaling and insulin expression. Conclusion: PI3K signaling must be activated for TR␣-induced reprogramming of pancreatic acinar cells. Significance: TR␣ is critical for postnatal expansion of the ␤-cell mass.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Ligand-bound Tr␣ Enhances the Expression Of Transcription Famentioning

confidence: 99%

See 1 more Smart Citation

Ligand-bound Thyroid Hormone Receptor Contributes to Reprogramming of Pancreatic Acinar Cells into Insulin-producing Cells

Furuya¹,

Shimura

Asami³

et al. 2013

Journal of Biological Chemistry

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“…cells can be stimulated to express Ngn3 [28,29] (Figure 2). This finding suggests that acinar cells can be a source of Ngn3 + precursors in the PDL model.…”

Section: Abbreviationsmentioning

confidence: 99%

The Quest for Tissue Stem Cells in the Pancreas and Other Organs, and their Application in Beta-Cell Replacement

Houbracken

Bouwens

2010

Rev Diabet Stud

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■ AbstractAdult stem cell research has drawn a lot of attention by many researchers, due to its medical hope of cell replacement or regenerative therapy for diabetes patients. Despite the many research efforts to date, there is no consensus on the existence of stem cells in adult pancreas. Genetic lineage tracing experiments have put into serious doubt whether β-cell neogenesis from stem/progenitor cells takes place postnatally. Different in vitro experiments have suggested centroacinar, ductal, acinar, stellate, or yet unidentified clonigenic cells as candidate β-cell progenitors. As in the rest of the adult stem cell field, sound and promising observations have been made. However, these observations still need to be replicated. As an alternative to committed stem/progenitor cells in the pancreas, transdifferentiation or lineage reprogramming of exocrine acinar and endocrine α-cells may be used to generate new β-cells. At present, it is unclear which approach is most medically promising. This article highlights the progress being made in knowledge about tissue stem cells, their existence and availability for therapy in diabetes. Particular attention is given to the assessment of methods to verify the existence of tissue stem cells.

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“…1 b-cell self-duplication, 2 acinar cell transdifferentiation [3][4][5] and pancreatic ductal cell reprogramming 6 have all been identified as potential pathways for islet regeneration in postnatal mammals. During embryogenesis, pancreatic progenitor/stem cells control the development of all pancreatic cells in the exocrine, endocrine and ductal compartments.…”

Section: Introductionmentioning

confidence: 99%

Ectopic transgenic expression of NKX2.2 induces differentiation of adult pancreatic progenitors and mediates islet regeneration

et al. 2012

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Notch Signaling as Gatekeeper of Rat Acinar-to-β-Cell Conversion in Vitro

Cited by 74 publications

References 45 publications

Ligand-bound Thyroid Hormone Receptor Contributes to Reprogramming of Pancreatic Acinar Cells into Insulin-producing Cells

Ligand-bound Thyroid Hormone Receptor Contributes to Reprogramming of Pancreatic Acinar Cells into Insulin-producing Cells

The Quest for Tissue Stem Cells in the Pancreas and Other Organs, and their Application in Beta-Cell Replacement

Ectopic transgenic expression of NKX2.2 induces differentiation of adult pancreatic progenitors and mediates islet regeneration

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