Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome

Masoudi, Mojtaba; Yamini, Nazila; Salehi, Fahimeh; Aflatoonian, Reza; Kutenaee, Maryam Azizi; Esfandiyari, Sahar; Hosseini, Elham; Asadi, Ebrahim; Najarkolaei, Azadeh Hosseini; Mortezaee, Keywan; Moini, Ashraf; Abolhassani, Farid; Salehi, Ensieh

doi:10.1007/s00404-021-06039-1

Cited by 8 publications

(6 citation statements)

References 32 publications

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“… 52 , 53 The Notch signaling pathway is associated with development and differentiation in all postnatal animals; it controls proper germ layer formation and embryonic segmentation and controls the timing and duration of differentiation events in a dynamic manner. 54 Studies have shown that the Notch pathway can lead to the loss of epithelial cell polarity and induces the onset of EMT in CRC. 55 The Notch pathway reduces the adhesion between epithelial cells by downregulating E-cadherin expression, which enhances the metastatic ability of CRC.…”

Section: Treatment and Drug Resistance Of Crc Associated With Emtmentioning

confidence: 99%

Research Progress of Epithelial-mesenchymal Transition Treatment and Drug Resistance in Colorectal Cancer

2022

Technol Cancer Res Treat

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Colorectal cancer (CRC) is one of the most common malignancies in the world that seriously affects human health. Activation of epithelial-mesenchymal transition (EMT) is a physiological phenomenon during embryonic development that is essential for cell metastasis. EMT participates in various biological processes associated with trauma repair, organ fibrosis, migration, metastasis, and infiltration of tumor cells. EMT is a new therapeutic target for CRC; however, some patients with CRC develop resistance to some drugs due to EMT. This review focuses specifically on the status of treatments that target the EMT process and its role in the therapeutic resistance observed in patients with CRC.

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Section: Treatment and Drug Resistance Of Crc Associated With Emtmentioning

confidence: 99%

Research Progress of Epithelial-mesenchymal Transition Treatment and Drug Resistance in Colorectal Cancer

2022

Technol Cancer Res Treat

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“…Existing findings about Notch in PCOS ovaries are quite limited and conflicting. Furthermore, most of them are based on transcriptome profiling of Notch and/or target genes, without examination of protein expression [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. In addition, all existing reports about granulosa cells utilized human GLCs or cumulus cells harvested at IVF after ovarian stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…Bioinformatic analysis showed that Notch signaling pathways are enriched in PCOS [ 20 ]. Notch1 and Notch2 mRNAs were upregulated in GLCs/cumulus cells of PCOS patients compared with those of control participants in some reports [ 15 , 18 ], while other reports showed that Notch2, Notch3, and Hes1 mRNAs were downregulated [ 16 , 17 , 19 ]. The present study showed that mRNA expression of Notch2 and target transcription factors of Notch2 signaling, Hey2 and Hes1, was increased in GLCs of PCOS patients.…”

Section: Discussionmentioning

confidence: 99%

“…It regulates follicular assembly and growth, steroidogenesis, and ovarian vascular development [ 11 ]. In addition to its physiological roles, recent studies demonstrated that Notch signaling components are aberrantly expressed in the human ovary in several pathologies including PCOS, although the findings are quite limited and conflicting [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Furthermore, the mechanisms that induce aberration of Notch signaling and the downstream pathological effects of its aberration remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Notch Signaling Induced by Endoplasmic Reticulum Stress Regulates Cumulus-Oocyte Complex Expansion in Polycystic Ovary Syndrome

et al. 2022

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Endoplasmic reticulum (ER) stress activated in granulosa cells contributes to the pathophysiology of polycystic ovary syndrome (PCOS). In addition, recent studies have demonstrated that Notch signaling plays multiple roles in the ovary via cell-to-cell interactions. We hypothesized that ER stress activated in granulosa cells of antral follicles in PCOS induces Notch signaling in these cells, and that activated Notch signaling induces aberrant cumulus-oocyte complex (COC) expansion. Expression of Notch2 and Notch-target transcription factors was increased in granulosa cells of PCOS patients and model mice. ER stress increased expression of Notch2 and Notch-target transcription factors in cultured human granulosa-lutein cells (GLCs). Inhibition of Notch signaling abrogated ER stress-induced expression of genes associated with COC expansion in cultured human GLCs, as well as ER stress-enhanced expansion of cumulus cells in cultured murine COCs. Furthermore, inhibition of Notch signaling reduced the areas of COCs in PCOS model mice with activated ER stress in the ovary, indicating that Notch signaling regulates COC expansion in vivo. Our findings suggest that Notch2 signaling is activated in granulosa cells in PCOS and regulates COC expansion. It remains to be elucidated whether aberrant COC expansion induced by the ER stress-Notch pathway is associated with ovulatory dysfunction in PCOS patients.

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“…Polycystic ovarian syndrome (PCOS), tubal factors, and unexplained infertility are among the primary causes of infertility [2]. PCOS is a common endocrine disorder with an incidence rate of about 4% to 8%, though it can be as high as 25% in some populations [3]. It has been noted that over 70% of women with PCOS exhibit normal-gonadotrophic anovulation [4].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with polycystic ovarian syndrome, tubal factor infertility, and unexplained infertility whose partners exhibit normal semen parameters: A retrospective study of sibling oocytes

Ashourzadeh,

Safari,

Hosseinisadat

et al. 2024

Clin Exp Reprod Med

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Objective: This study compared the outcomes of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in patients with polycystic ovarian syndrome (PCOS), tubal factor (TF) infertility, and unexplained infertility whose partners had normal semen parameters.Methods: This retrospective study included 360 couples diagnosed with infertility involving PCOS (n=157), unexplained infertility (n=140), and TF infertility (n=63). Sibling oocytes were randomly assigned to undergo ICSI or conventional IVF insemination. The fertilization rate and embryo morphology were evaluated as outcomes. Results: Retrieved cumulus-oocyte complexes from patients with PCOS (2,974), unexplained infertility (1,843), and TF infertility (844) were split and inseminated by conventional IVF and ICSI respectively. In comparison to the ICSI method, the conventional IVF approach was linked to a significantly higher fertilization rate in groups with PCOS (68.81% vs. 77.49%), unexplained infertility (67.62% vs. 78.84%), and TF issues (69.23% vs. 78.63%) (p<0.05). The proportion of embryos with grade A produced by the conventional IVF method was significantly higher than that produced using the ICSI method in the PCOS and unexplained infertility groups (p<0.05). Additionally, the percentage of grade B embryos produced with the ICSI method was significantly higher than that produced with the conventional IVF method in PCOS patients (p=0.002).Conclusion: Our results indicated that the conventional IVF method was associated with higher zygote production and a higher proportion of grade A embryos when all infertile groups were evaluated together. Thus, ICSI is not suggested for patients with these causes of infertility if their partner has normal semen parameters.

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Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome

Cited by 8 publications

References 32 publications

Research Progress of Epithelial-mesenchymal Transition Treatment and Drug Resistance in Colorectal Cancer

Research Progress of Epithelial-mesenchymal Transition Treatment and Drug Resistance in Colorectal Cancer

Notch Signaling Induced by Endoplasmic Reticulum Stress Regulates Cumulus-Oocyte Complex Expansion in Polycystic Ovary Syndrome

Comparative analysis of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with polycystic ovarian syndrome, tubal factor infertility, and unexplained infertility whose partners exhibit normal semen parameters: A retrospective study of sibling oocytes

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