NOTE: Elevation of Mycoplasma agalactiae subsp. bovis to Species Rank: Mycoplasma bovis (Hale et al.) comb. nov.

Askaa, Gerd; Ernø, H.

doi:10.1099/00207713-26-3-323

Cited by 65 publications

(35 citation statements)

References 7 publications

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“…MALDI-TOF MS also proved to accurately distinguish species that are known to be closely related, M. pneumoniae, M. genitalium, and M. amphoriforme, which grow in the same HG medium, and M. bovis and M. agalactiae, which used to be classified as two subspecies of the same species prior to being separated into two different species based on serological, DNA-DNA reassociation experiments (41), and 16S rRNA sequence data (42). Furthermore, within individual species, the dendrograms generated from the MALDI-TOF MS spectra achieved epidemiologically relevant strain clustering.…”

Section: Discussionmentioning

confidence: 99%

Identification and Subtyping of Clinically Relevant Human and Ruminant Mycoplasmas by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2013

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g Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) recently emerged as a technology for the identification of bacteria. In this study, we aimed to evaluate its applicability to human and ruminant mycoplasmal identification, which can be demanding and time-consuming when using phenotypic or molecular methods. In addition, MALDI-TOF MS was tested as a subtyping tool for certain species. A total of 29 main spectra (MSP) from 10 human and 13 ruminant mycoplasma (sub)species were included in a mycoplasma MSP database to complete the Bruker MALDI Biotyper database. After broth culture and protein extraction, MALDI-TOF MS was applied for the identification of 119 human and 143 ruminant clinical isolates that were previously identified by antigenic or molecular methods and for subcultures of 73 ruminant clinical specimens that potentially contained several mycoplasma species. MALDI-TOF MS resulted in accurate (sub)species-level identification with a score of >1.700 for 96% (251/262) of the isolates. The phylogenetically closest (sub)species were unequivocally distinguished. Although mixtures of the strains were reliably detected up to a certain cellular ratio, only the predominant species was identified from the cultures of polymicrobial clinical specimens. For typing purposes, MALDI-TOF MS proved to cluster Mycoplasma bovis and Mycoplasma agalactiae isolates by their year of isolation and genome profiles, respectively, and Mycoplasma pneumoniae isolates by their adhesin P1 type. In conclusion, MALDI-TOF MS is a rapid, reliable, and cost-effective method for the routine identification of high-density growing mycoplasmal species and shows promising prospects for its capacity for strain typing.

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Section: Discussionmentioning

confidence: 99%

Identification and Subtyping of Clinically Relevant Human and Ruminant Mycoplasmas by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2013

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“…Hale et al (1962) relataram o primeiro caso de mastite bovina por Mycoplasma agalactiae subsp. bovis, posteriormente denominado por Aska & Erno (1976) de Mycoplasma bovis. No Brasil, a primeira citação de mastite por M. bovis foi de Mettifogo et al (1996) na região de Londrina, Estado do Paraná.…”

Section: Introductionunclassified

Mastite bovina por Mycoplasma bovis em rebanhos leiteiros

et al. 2001

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Foram examinadas 713 vacas de três rebanhos leiteiros localizados na região norte do Estado do Paraná e sudoeste do Estado de São Paulo, das quais 137 apresentaram mastite. Nas três propriedades foram detectados oito animais (1,12%) com mastite clínica por Mycoplasma bovis. Destes animais, quatro tratados com oxitetraciclina e tilosina e três com enrofloxacina, não responderam ao tratamento e foram descartados no decorrer da lactação. Uma vaca medicada com enrofloxacina recuperou quase que totalmente a secreção láctea mas a eliminação de M. bovis persistiu por toda lactação. Esta vaca apresentou cura bacteriológica na lactação seguinte. O descarte dos animais positivos, monitora-mento bacteriológico e a aplicação correta das medidas de prevenção para as mastites contagiosas controlaram a disseminação de M. bovis nos rebanhos.

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“…The extent of DDH, although recently demoted as an absolute arbiter of species status (Rosselló -Mora & Amann, 2001), may provide essential justification for promoting a strain group to subspecies status or elevation of a subspecies to species rank (Askaa & Ernø, 1976). A subspecies should not be proposed if serological and supporting phenotypic or ecological evidence is limited or if the strain has few cohorts.…”

Section: (V) Adherence To Taxonomic Rules and Proceduresmentioning

confidence: 99%

“…Cumbersome and difficult to standardize though it may be, this technique remains an important benchmark in assessment of species status in prokaryote taxonomy (Grimont, 1988;Murray et al, 2001;Stackebrandt et al, 2002). DDH experiments may be necessary particularly when designating subspecies or elevating subspecies to species status (Askaa & Ernø, 1976;Bradbury et al, 1993;Leach et al, 1993).…”

Section: (Xii) Genomic and Genetic Analysesmentioning

confidence: 99%

Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes)

Brown

Whitcomb

Bradbury

2007

International Journal of Systematic and Evolutionary Microbiology

138

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Minimal standards for novel species of the class Mollicutes (trivial term, mollicutes), last published in 1995, require revision. The International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Mollicutes proposes herein revised standards that reflect recent advances in molecular systematics and the species concept for prokaryotes. The mandatory requirements are: (i) deposition of the type strain into two recognized culture collections, preferably located in different countries; (ii) deposition of the 16S rRNA gene sequence into a public database, and a phylogenetic analysis of the relationships among the 16S rRNA gene sequences of the novel species and its neighbours; (iii) deposition of antiserum against the type strain into a recognized collection; (iv) demonstration, by using the combination of 16S rRNA gene sequence analyses, serological analyses and supplementary phenotypic data, that the type strain differs significantly from all previously named species; and (v) assignment to an order, a family and a genus in the class, with an appropriate specific epithet. The 16S rRNA gene sequence provides the primary basis for assignment to hierarchical rank, and may also constitute evidence of species novelty, but serological and supplementary phenotypic data must be presented to substantiate this. Serological methods have been documented to be congruent with DNA-DNA hybridization data and with 16S rRNA gene placements. The novel species must be tested serologically to the greatest extent that the investigators deem feasible against all neighbouring species whose 16S rRNA gene sequences show .0.94 similarity. The investigator is responsible for justifying which characters are most meaningful for assignment to the part of the mollicute phylogenetic tree in which a novel species is located, and for providing the means by which novel species can be identified by other investigators.

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NOTE: Elevation of Mycoplasma agalactiae subsp. bovis to Species Rank: Mycoplasma bovis (Hale et al.) comb. nov.

Cited by 65 publications

References 7 publications

Identification and Subtyping of Clinically Relevant Human and Ruminant Mycoplasmas by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Identification and Subtyping of Clinically Relevant Human and Ruminant Mycoplasmas by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Mastite bovina por Mycoplasma bovis em rebanhos leiteiros

Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes)

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