Note: Mobile micro-Raman analyzer integrated with a lab-on-a-chip

Abstract

A mobile micro-Raman microfluidic analyzer was designed and built for label-free, nondestructive, fingerprint detection of samples on microfluidic chip systems. It mainly includes an optical module (including the Raman detection system and the microscopic imaging system), 3-axis stages with step motors and other auxiliary circuits. The setup of the analyzer was designed with fully considering the characters of the microfluidics system. The experimental results prove that this microfluidics analyzer is practical and very convenient to use. This micro-Raman microfluidics analyzer with high performance to cost ratio has wide application potential in lab-on-a-chip fields as a powerful analytical tool.

A highly sensitive microfluidics system for multiplexed surface-enhanced Raman scattering (SERS) detection based on Ag nanodot arrays

“…Scheme 1(b) shows the detailed conguration of the detection zone of the microuidic chip. Subsequently, the nished microuidic chip was installed on a self-built Raman microspectrometer (Supra-1 model) 17 for SERS detection (Scheme 1(c)). Scheme 1(d) and (e) show the results of structure characterizations of Ag nanodots with good uniformity.…”

“…Our research group has reported a micro-Raman analyser that copes with this problem in our previous publication. 17 The reported Raman micro-analyser integrates an inverted microscopic imaging system and a Raman detection system for Raman experiments, with a 3-axis stage controlled by step motors for easy positioning of the laser spot onto the detection zone. Furthermore, the optimized setup of the micro-Raman analyser was designed under the consideration of microuidic chips that are oen capped with moulded channels, so the inverted micro-imaging system and the Raman system were under the microuidic chips, minimizing the interference brought about by the cumbersome PDMS cover and circulation tubes, while in the meantime retaining SERS enhancement ability of the substrate during detection.…”

“…11,[13][14][15][16] Nevertheless, it is even harder for a commercial Raman solution to focus inside the microuidic channel, because the existence of a polymer cover may contaminate the Raman signals of the analytes, and the circulation tubes may be too cumbersome for SERS detection. Therefore, we employed a self-developed Raman microanalyser equipped with an inverted laser path and an XYZaxis-moveable microscopic imaging and detection system 17 for measuring this SERS microuidic chip, easily focusing laser spots and performing on-site, real-time SERS detection.…”

Qualitative analysis of desi ghee, edible oils, and spreads using Raman spectroscopy

“…Raman micro‐spectroscopy has been used for the identification of microorganism . It is now being considered a suitable detection probe with lab‐on‐chip to produce an efficient clinical diagnostic tool . Recently, it has been reported for the prediction of dengue virus infection and plasmodium viral infection in human sera/plasma .…”

ZnO/Ag nanoarrays substrate for surface-enhanced Raman scattering (SERS) detection

“…Various methods for the preparation of SERS substrates have been reported, such as atomic layer deposition (ALD) [8], chemical reduction [9], lithography and ion-beamsputtered technique. There are a variety of forms of SERS substrates, such as hydrothermal Au/Ag nano colloids [10], Au/Ag nanoparticles assembled films [11][12][13][14], semi-conductor substrates [15,16] and anodized aluminum oxide templates [17], and so on. One-dimensional nanoscale metal-semiconductor composite materials exhibit distinctive optical, electronic and chemical properties and other interesting applications in catalysis, biomedicine and photonics.…”