NOTES: The <i>DAL81</i> Gene Product Is Required for Induced Expression of Two Differently Regulated Nitrogen Catabolic Genes in Saccharomyces cerevisiae

Bricmont, P A; Daugherty, Jon R.; Cooper, Terrance G.

doi:10.1128/mcb.11.2.1161-1166.1991

Cited by 11 publications

(3 citation statements)

References 34 publications

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“…Tup1 and Ssn6 form a corepressor complex in yeast and have a known role in regulating amino acid permeases (Tanaka and Mukai 2015), while Stp2 is an amino acid regulated TR that regulates amino acid permeases (Martínez and Ljungdahl 2005). Dal81 has a known role in positively regulating nitrogen degradation in S. cerevisiae (Bricmont et al 1991), but has a different role in regulating arginine synthesis in C. parapsilosis (Turner et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Transcriptional regulation of the synthesis and secretion of farnesol in the fungusCandida albicans:Examination of the Homann transcription regulator knockout collection

Gutzmann

Kramer

Toomey

et al. 2023

Preprint

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Candida albicansis an efficient colonizer of human gastrointestinal tracts and skin and is an opportunistic pathogen.C. albicansexhibits morphological plasticity and the ability to switch between yeast and filamentous morphologies is associated with virulence. One regulator of this switch is the quorum sensing molecule farnesol which is produced inC. albicanscells throughout growth. In addition, aromatic fusel alcohols accumulate inC. albicanssupernatants and are implicated in regulating morphogenesis. The synthesis, secretion, and turnover of these molecules is not fully understood. To address this, we used our improved assay for farnesol and aromatic fusel alcohol quantification of whole cultures, pellets, and supernatants, to screen a transcription regulator knockout library of 164 mutants for differences in farnesol and aromatic fusel alcohol accumulation and localization. We found that all mutants produced farnesol with an average of 9.2X more farnesol localizing to the pellet in most mutants. Nineteen mutants had significant differences in accumulation and nine differed in localization. Ten mutants produced more farnesol than their SN152+parent, while nine produced less. Seven mutants exhibited greater secretion of farnesol while two exhibited less. Several of these mutants have known roles in white/opaque switching and cell cycle progression such as the farnesol overproducing mutantsahr1andswi4. The aromatic fusel alcohols were similarly characterized by four over- and eleven under-producing mutants. Phenethyl alcohol and tryptophol accumulation were closely proportional to one another, but despite their structural similarity, phenethyl alcohol and tyrosol accumulation were often regulated differently.

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Section: Resultsmentioning

confidence: 99%

Transcriptional regulation of the synthesis and secretion of farnesol in the fungusCandida albicans:Examination of the Homann transcription regulator knockout collection

Gutzmann

Kramer

Toomey

et al. 2023

Preprint

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“…Although the DBD’s metal biding portion is essential for the DNA binding activity, it can be dispensable in some cases and still exerts its regulatory activity [ 15 ]. In particular, the expression of a truncated-zinc Zn 2 Cys 6 finger motif DAL81 allele in a dal81 S. cerevisiae strain recovers its ability to induce the urea amidolyase activity, encoded by the Dal81p-regulated DUR1 , 2 gene [ 47 ]. The same was observed when the expression of a truncated DAL81 allele lacking the six cysteine residues of the Zn 2 Cys 6 finger motif could recover the ability of a dal81 mutant to express the β-galactosidase reporter under the control of the GABA upstream activation sequence (UAS GABA ), which is present in the promoters of both Dal81p- and Uga3p-regulated genes UGA1 and UGA4 [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

A novel aminotransferase gene and its regulator acquired in Saccharomyces by a horizontal gene transfer event

et al. 2023

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Background Horizontal gene transfer (HGT) is an evolutionary mechanism of adaptive importance, which has been deeply studied in wine S. cerevisiae strains, where those acquired genes conferred improved traits related to both transport and metabolism of the nutrients present in the grape must. However, little is known about HGT events that occurred in wild Saccharomyces yeasts and how they determine their phenotypes. Results Through a comparative genomic approach among Saccharomyces species, we detected a subtelomeric segment present in the S. uvarum, S. kudriavzevii, and S. eubayanus species, belonging to the first species to diverge in the Saccharomyces genus, but absent in the other Saccharomyces species. The segment contains three genes, two of which were characterized, named DGD1 and DGD2. DGD1 encodes dialkylglicine decarboxylase, whose specific substrate is the non-proteinogenic amino acid 2-aminoisobutyric acid (AIB), a rare amino acid present in some antimicrobial peptides of fungal origin. DGD2 encodes putative zinc finger transcription factor, which is essential to induce the AIB-dependent expression of DGD1. Phylogenetic analysis showed that DGD1 and DGD2 are closely related to two adjacent genes present in Zygosaccharomyces. Conclusions The presented results show evidence of an early HGT event conferring new traits to the ancestor of the Saccharomyces genus that could be lost in the evolutionary more recent Saccharomyces species, perhaps due to loss of function during the colonization of new habitats.

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“…Induction of this permease requires at least two positive acting proteins, the specifc Uga3p factor and the pleiotropic Uga35p factor (i.e. Dal81p/DurLp) [14,15]. These factors act through a 19 bp CG-rich upstream activating sequence, UAS GABA .…”

Section: Introductionmentioning

confidence: 99%

UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene

Moretti

Batlle

García

2001

The International Journal of Biochemistry &Amp; Cell Biology

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NOTES: The DAL81 Gene Product Is Required for Induced Expression of Two Differently Regulated Nitrogen Catabolic Genes in Saccharomyces cerevisiae

Cited by 11 publications

References 34 publications

Transcriptional regulation of the synthesis and secretion of farnesol in the fungusCandida albicans:Examination of the Homann transcription regulator knockout collection

Transcriptional regulation of the synthesis and secretion of farnesol in the fungusCandida albicans:Examination of the Homann transcription regulator knockout collection

A novel aminotransferase gene and its regulator acquired in Saccharomyces by a horizontal gene transfer event

UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene

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