Nothobranchius as a model for anorexia of aging research: an evolutionary, anatomical, histological, immunohistochemical, and molecular study

Mhalhel, Kamel; Briglia, Marilena; Aragona, Marialuisa; Porcino, Caterina; Abbate, Francesco; Guerrera, Maria Cristina; Laurà, Rosaria; Krichen, Youssef; Guerbej, Hamadi; Germanà, Antonino; Levanti, Maria; Montalbano, Giuseppe

doi:10.1016/j.aanat.2023.152116

Cited by 6 publications

(6 citation statements)

References 63 publications

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“…The pathological observation of the intestinal tissue of zebrafish was examined by using the hematoxylin and eosin (H&E) [ 48 ] and Alcian blue (AB) [ 49 ] methods. In this experiment, AB zebrafish embryos at 3 dpf were randomly divided into six groups and colitis zebrafish was induced as described in Section 4.4 .…”

Section: Methodsmentioning

confidence: 99%

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The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

Cao,

Xu,

Xia

et al. 2024

Marine Drugs

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Inflammatory bowel disease (IBD) is a chronic inflammatory bowel disease with unknown pathogenesis which has been gradually considered a public health challenge worldwide. Peptides derived from Rapana venosa have been shown to have an anti-inflammatory effect. In this study, peptide LLTRAGL derived from Rapana venosa was prepared by a solid phase synthesis technique. The protective effects of LLTRAGL were studied in a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced zebrafish colitis model. The underlying mechanisms of LLTRAGL were predicted and validated by transcriptome, real-time quantitative PCR assays and molecular docking. The results showed that LLTRAGL reduced the number of macrophages migrating to the intestine, enhanced the frequency and rate of intestinal peristalsis and improved intestinal inflammatory damage. Furthermore, transcriptome analysis indicated the key pathways (NOD-like receptor signal pathway and necroptosis pathway) that link the underlying protective effects of LLTRAGL’s molecular mechanisms. In addition, the related genes in these pathways exhibited different expressions after TNBS treatment. Finally, molecular docking techniques further verified the RNA-sequencing results. In summary, LLTRAGL exerted protective effects in the model of TNBS-induced colitis zebrafish. Our findings provide valuable information for the future application of LLTRAGL in IBD.

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Section: Methodsmentioning

confidence: 99%

“…The ultrastructure of the intestinal tissue of zebrafish was observed by using the scanning electron microscopy method [ 49 ]. After drug treatment, ten zebrafish larvae were randomly collected from each group and fixed in an electron microscope fixation liquid containing 5% glutaraldehyde.…”

Section: Methodsmentioning

confidence: 99%

The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

Cao,

Xu,

Xia

et al. 2024

Marine Drugs

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“…The immunofluorescence technique was used to evaluate the expression of BDNF in the larvae telencephalon sections as previously described [ 14 ]. In brief, sections were rinsed in Tris-HCl buffer (0.05 M, pH 7.5) containing bovine serum albumin and Triton-X 100.…”

Section: Methodsmentioning

confidence: 99%

“…During the past decade, teleost fish have been introduced as successful vertebrate models in scientific research [ 14 , 15 , 16 , 17 ], emphasizing zebrafish species ( Danio rerio ) [ 18 , 19 ]. Zebrafish offer multifaceted advantages in developmental neurotoxicity testing, as seen in the concordance between zebrafish and human neurodevelopmental pathways reported in many studies [ 5 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Urtica dioica Extract Abrogates Chlorpyrifos-Induced Toxicity in Zebrafish Larvae

Mhalhel,

Kadmi,

Ben Chira

et al. 2024

IJMS

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Chlorpyrifos (CPF) is a widely used organophosphate insecticide, though its excessive use causes environmental contamination, raising concerns about its adverse effects on human health. In this regard, Urtica dioica stands out as a promising candidate for counteracting chemical ‘contaminant’ toxicity thanks to its therapeutic properties. Therefore, our study aimed to investigate the potential of an Urtica dioica ethanolic extract (UDE) to mitigate chlorpyrifos-induced toxicity. Eight compounds in the Urtica dioica ethanolic extract have been identified, most of which present significant potential as antioxidant, anti-inflammatory, and neuroprotective agents. Chlorpyrifos exposure altered hatching rates, increased the incidence of teratogenic effects, and upregulated the expression of brain-derived neurotrophic factor (Bdnf) in zebrafish larvae telencephalon. On the other hand, UDE demonstrated a preventive effect against CPF-induced teratogenicity, which is expressed by a lower morphological deformity rate. Moreover, the UDE showed a rather protective effect, maintaining the physiological condition of the telencephalon. Additionally, CPF altered the locomotor behavior of larvae, which was characterized by irregular swimming and increased activity. This defective behavioral pattern was slightly attenuated by the UDE. Our findings suggest that the UDE possesses significant protective properties against CPF-induced toxicity, probably conferred by its natural antioxidant and anti-inflammatory contents. Still, further research is needed to elucidate the recruited mechanisms and implicated pathways on UDE’s protective effects.

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“…In addition, the Nothobranchius retina undergoes neurogenesis and regeneration phenomena, even in postnatal life [ 24 ], thanks to the persistence of stem cells. For all these reasons, the Nothobranchius is being established as a model organism in aging research [ 42 ], which has been impaired for a long time in vertebrates due to the lack of short-lived models [ 36 ]. N. guentheri is not only a suitable object of study in the fields of evolution and development, but has recently also been proposed as a model for studying the structure, formation, and stages of the onset of age-related changes in the visual system [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Neurotrophins and Trk Neurotrophin Receptors in the Retina of Adult Killifish (Nothobranchius guentheri)

Porcino,

Mhalhel,

Briglia

et al. 2024

IJMS

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Specific subpopulations of neurons in nerve and sensory systems must be developed and maintained, and this is accomplished in significant part by neurotrophins (NTs) and the signaling receptors on which they act, called tyrosine protein kinase receptors (Trks). The neurotrophins–tyrosine protein kinase receptors (NTs/Trks) system is involved in sensory organ regulation, including the visual system. An NTs/Trks system alteration is associated with neurodegeneration related to aging and diseases, including retinal pathologies. An emergent model in the field of translational medicine, for instance, in aging study, is the annual killifish belonging to the Nothobranchius genus, thanks to its short lifespan. Members of this genus, such as Nothobranchius guentheri, and humans share a similar retinal stratigraphy. Nevertheless, according to the authors’ knowledge, the occurrence and distribution of the NTs/Trks system in the retina of N. guentheri has never been investigated before. Therefore, the present study aimed to localize neurotrophin BDNF, NGF, and NT-3 and TrkA, TrkB, and TrkC receptors in the N. guentheri retina using the immunofluorescence method. The present investigation demonstrates, for the first time, the occurrence of the NTs/Trks system in N. guentheri retina and, consequently, the potential key role of these proteins in the biology and survival of the retinal cells.

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Nothobranchius as a model for anorexia of aging research: an evolutionary, anatomical, histological, immunohistochemical, and molecular study

Cited by 6 publications

References 63 publications

The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

Urtica dioica Extract Abrogates Chlorpyrifos-Induced Toxicity in Zebrafish Larvae

Neurotrophins and Trk Neurotrophin Receptors in the Retina of Adult Killifish (Nothobranchius guentheri)

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