Notice of Retraction: Homology Modeling and Expression Analysis of Sterol 14alpha-Demethylase from Ustilago maydis

Abstract

Corn smut is one of the most serious diseases affecting corn crops caused by Ustilago maydis. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis and has been shown to be an effective target of antifungal drugs. To design novel antifungal compounds against UmCYP51, in this study, homology modeling and expression of CYP51 from U. maydis (UmCYP51) were carried out. A-three-dimensional structure model of UmCYP51 was built through homology modeling based on the crystal structure of Mycobacterium tuberculosis. Only pET32-Um-35 could be highly expressed in E. coli BL21 (DE3)successfully after many attempts, which indicated N terminal transmembrane regions and the lowest energy of mRNA structure are importment for the protein expression of UmCYP51, combining of bioinformatics analysis of transmembrane prediction and mRNA secondary structure of translation initiation region. These results will provide some information for predicting the expression of protein from the angle of bioinformatics, and will set the stage for insighting the feature of the UmCYP51.

Fungicides Inhibition Analysis by Molecular Docking and Sensitivity Testing of <i>Penicillium italicum</i>

“…The 3D model of PiCYP51 obtained from NCBI database (accession number: CAA89824) has been built yet [10]. The amino acid sequence of PiCYP51 in this study was obtained from the isolate (HS-1).…”