Notice of Violation of IEEE Publication Principles: Peptide coated quantum dots for biological applications

Iyer, Gopal; Pinaud, Fabien; Tsay, James M.; Li, J.J.; Bentolila, Laurent A.; Michalet, Xavier; Weiss, Shimon

doi:10.1109/tnb.2006.886563

Cited by 16 publications

(5 citation statements)

References 43 publications

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“…Previously, we reported a ligand exchange approach which is based on phytochelatin-related α-peptide ligands (“peptide coating”) 25–28 . These peptides contained multiple cysteines interspaced by hydrophobic residues and were anchored to Zn or Cd ions on the QD surface via the cysteines’ thiol groups.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we reported a ligand exchange approach which is based on phytochelatin-related α-peptide ligands (“peptide coating”). − These peptides contained multiple cysteines interspaced by hydrophobic residues and were anchored to Zn or Cd ions on the QD surface via the cysteines’ thiol groups. The α-peptide coating provided the QDs with excellent colloidal properties and allowed to modulate their charge, solubility, and functionality via the peptide sequence itself.…”

Section: Introductionmentioning

confidence: 99%

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Stable, Compact, Bright Biofunctional Quantum Dots with Improved Peptide Coating

Ruchala

Ebenstain

et al. 2012

J. Phys. Chem. B

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We developed a new peptide, natural phytochelatin (PC), which tightly binds to CdSe/ZnS quantum dots’ (QDs) surfaces and renders them water-soluble. Coating QDs with this flexible and all-hydrophilic peptide offers high colloidal stability, adds only 0.8–0.9 nm to the radius of the particles (as compared to their original inorganic radius), preserves very high quantum yield (QY) in water, and affords facile bioconjugation with various functional groups. We demonstrate specific targeting (with minimal non-specific binding) of such fluorescein-conjugated QDs to ScFv-fused mouse prion protein expressed in live N2A cells. We also demonstrated homogeneous in-vivo biodistribution with no significant toxicity in live zebrafish.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stable, Compact, Bright Biofunctional Quantum Dots with Improved Peptide Coating

Ruchala

Ebenstain

et al. 2012

J. Phys. Chem. B

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“…Researchers have encountered difficulties in developing QD surface passivation coatings that simultaneously satisfy all of the above criteria. Published methods in the literature include the use of monothiolated [11][12][13] or bidentate thiols [14], silane [6,15], encapsulation within amphiphilic polymers [16][17][18][19], and the direct attachment of proteins or peptides [20][21][22][23]. Comprehensive reviews on QDs are available that describe further details of surface coatings and capping strategies [3,24,25].…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies attempting to quantitatively evaluate a particular passivation strategy, quantum yield (QY) has been used as the primary figure-of-merit, with most researchers determining the QY with, and without the passivating ligand [15,22,31]. Researchers have additionally used parameters including cytotoxicity [32], luminescence intensity over time [28,33], and colloidal stability across a range of pH [28,34].…”

Section: Introductionmentioning

confidence: 99%

Photostability of quantum dots with amphiphilic polymer-based passivation strategies

Nida

Nitin

et al. 2007

Nanotechnology

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Quantum dots (QDs) have many appealing properties for biological fluorescence imaging, but exhibit photostabilities that are dependent upon surface passivation to minimize susceptibility to oxygen and light. Here, through spectroscopy and imaging techniques, we compare the photostability of micelle-encapsulated QDs with QDs passivated with either crosslinked amphiphilic polymers or crosslink-free amphiphilic polymers. Both crosslinked and crosslink-free amphiphilic polymer passivation strategies produced QDs with high photoluminescence stability for exposure to light under ambient conditions. In contrast, micelle encapsulation resulted in QDs with photoluminescence emission levels that were highly sensitive to both light exposure and oxygen, exhibiting a reduction of up to 70% in photoluminescence intensity within twenty minutes of exposure. With the addition of reducing agents, the photoluminescence level of the micelle-encapsulated QDs was significantly stabilized. We conclude that amphiphilic polymers provide coatings with considerably higher integrity and stability than micelle encapsulation, reducing the QDs' sensitivities to oxygen and light, both of which are relevant factors in biological imaging applications.

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“…Detection of bio-molecules in many cases is assisted by labels or 'tags' [38][39][40][41][42][43] as mentioned above to allow for very sensitive detection even down to the level of single molecules [44][45][46]. However, labels can structurally and functionally interfere with an assay, and require nontrivial biochemical engineering for proper performance.…”

Section: Vcsels For Label-free Biosensingmentioning

confidence: 99%

Emerging applications for vertical cavity surface emitting lasers

Harris¹,

O’Sullivan²,

Sarmiento³

et al. 2010

Semicond. Sci. Technol.

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Vertical cavity surface emitting lasers (VCSELs) emitting at 850 nm have experienced explosive growth in the past decade because of their many attractive optical features and incredibly low-cost manufacturability. This review reviews the foundations for GaAs-based VCSEL technology as well as the materials and device challenges to extend the operating wavelength to both shorter and longer wavelengths. We discuss some of the applications that are enabled by the integration of VCSELs with both active and passive semiconductor elements for telecommunications, both in vivo and in vitro biosensing, high-density optical storage and imaging at wavelengths much less than the diffraction limit of light.

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Notice of Violation of IEEE Publication Principles: Peptide coated quantum dots for biological applications

Cited by 16 publications

References 43 publications

Stable, Compact, Bright Biofunctional Quantum Dots with Improved Peptide Coating

Stable, Compact, Bright Biofunctional Quantum Dots with Improved Peptide Coating

Photostability of quantum dots with amphiphilic polymer-based passivation strategies

Emerging applications for vertical cavity surface emitting lasers

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