Novel 3D-printed methacrylated chitosan-laponite nanosilicate composite scaffolds enhance cell growth and biomineral formation in MC3T3 pre-osteoblasts

Cebe, Tugba; Ahuja, Neelam; Monte, Felipe; Awad, Kamal; Vyavhare, Kimaya; Aswath, Pranesh B.; Huang, Jian; Brotto, Marco; Varanasi, Venu

doi:10.1557/jmr.2018.260

Cited by 55 publications

(37 citation statements)

References 56 publications

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“…Recently, synthetic silicates have exposed a high degree of functionality for promoting osteodifferentiation and bone healing 7 . Laponite (LAP, 59.5% SiO 2 , 27.5% MgO, 2.8% Na 2 O, and 0.8% Li 2 O) is synthetic disk‐shaped nanoplatelets with approximately 25 nm diameter and 1 nm thickness 8 . LAP nanoplatelets introduced as a bioactive nanomaterial to amend the mechanical properties and osteoinductivity of scaffolds without external osteoinductive factors 9 …”

Section: Introductionmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

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Section: Introductionmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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“…In this regard, HA is one of the most common ceramics which is used for this application [82]. Likewise, in numerous researches CS is incorporated into the synthetic-based polymer, including PCL, PLLA [85][86][87][88][89][90][91], or synthetic polymers and ceramics are incorporated at the same time [92][93][94][95]. Even though synthetic polymers offer positive characteristics including cytocompatibility and sufficient mechanical performance, their hydrophobicity and the absence of particular cell recognition sites restrict their functional application.…”

Section: Bone Regenerationmentioning

confidence: 99%

“…These results indicate that CS embedded into PCL scaffolds improved osteogenesis of BMMSCs for seven days, as opposed to pure PCL scaffolds. Keeping that in mind, at 14 days, CS and CS-embedded PCL constructs portrayed incredibly higher levels of (Alkaline phosphatase) ALP, (osteocalcin) OCN, and (collagen) COL1 compared with pure PCL scaffolds [85,[92][93][94][95].…”

Section: Bone Regenerationmentioning

confidence: 99%

“…By implantation of scaffolds in mice, ARS revealed just disperse calcium deposition in the CSG specimen and restricted calcium deposition on the PCL scaffold fibers. On the other hand, more substantial area of ARS was formed in the pores and fibers of hybrid scaffolds [ 85 , 92 , 93 , 94 , 95 ]. Furthermore, it was exhibited that the pore sizes >300 μm are generally helpful for improved fresh bone generation and the creation of capillaries.…”

Section: Chitosan-based 3d Printed Construct For Hard Tissues Applmentioning

confidence: 99%

“…Taken together, the results illustrated the significant effect of CS in the enhancement of osteogenesis level, whereas poor mechanical properties limited the application of the CS polymer for 3D printing. However, after incorporation of HA [ 83 , 96 ], calcium phosphate (Ca-P) [ 84 ], laponite [ 93 ], and bioglass (BG) [ 97 ] into the natural polymers, the ability of CS scaffolds for 3D printing for bone repair was remarkably escalated. In addition to the mechanical properties, incorporating various types of materials into each other helps to achieve optimal rheology, the parameter which can influence the printability of the compounds used as the slurry for constructs made by 3D printing.…”

Section: Chitosan-based 3d Printed Construct For Hard Tissues Applmentioning

confidence: 99%

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Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

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Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

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Utilizing the Sensitization Effect for Direct Laser Writing in a Novel Photoresist Based on the Chitin Monomer N‐acetyl‐D‐glucosamine

Meiers¹,

Rothammer

Maier

et al. 2023

Adv Eng Mater

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Novel 3D-printed methacrylated chitosan-laponite nanosilicate composite scaffolds enhance cell growth and biomineral formation in MC3T3 pre-osteoblasts

Abstract: Abstract

Cited by 55 publications

References 56 publications

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

Utilizing the Sensitization Effect for Direct Laser Writing in a Novel Photoresist Based on the Chitin Monomer N‐acetyl‐D‐glucosamine

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