Novel Allele of FAD2-1A from an EMS-Induced Mutant Soybean Line (PE529) Produces Elevated Levels of Oleic Acid in Soybean Oil

Jo, Hyun; Woo, Changwan; Norah, Nabachwa; Song, Jong Tae; Lee, Jeong‐Dong

doi:10.3390/agronomy12092115

Cited by 6 publications

(3 citation statements)

References 38 publications

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“…The new allele FAD2-1A was induced by Jo et al in soybean mutant PE529; they showed that oleic acid content in soybean oil can be increased to 80%. The results were significantly higher than those of this experiment, which may be due to the influence of different soybean lines on genetic factors and different experimental environments [29]. Due to the different levels of expression, and the inhibition of the FAD2 gene family in different plants [30], the degree of increase in the oleic acid content varies.…”

Section: Discussioncontrasting

confidence: 64%

Construction and Functional Evaluation of CRISPR/Cas9 Multiple Knockout Vectors of the FAD2 Gene Family

Zhang

Xiao

et al. 2023

Agronomy

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Soybean oil is a traditional edible oil. Increasing the oleic acid content is an important direction of soybean breeding. The soybean FAD2 family consists of seven genes that regulate how oleic acid is converted into linoleic acid. Five genes of the soybean FAD2 gene family, GmFAD2-1B, GmFAD2-1A, GmFAD2-2B, GmFAD2-2C, and GmFAD2-2D, were taken as target genes in this study. Firstly, a multivalent CRISPR/Cas9 gene-editing vector was constructed to regulate FAD2 gene expression. Multiple knockout vectors were inserted into the soybean varieties JN38, T6098, and T7010 using Agrobacterium-mediated soybean cotyledon transformation. The functional analysis, agronomic character analysis, and comparison of the mutant lines of the offspring of different genotypes indicated that the JN38 mutant was significantly taller in terms of plant height than the receptor JN38. The fatty acid content of the three groups showed the same trend. The fatty acid contents of mutant plants were higher than those of recipient plants, and the linoleic acid contents of mutant plants were lower than those of recipient plants. The best-performing among the three groups was the JN38 mutant, whose oleic acid content increased from 18.58% to 54.07% and whose linoleic acid content decreased from 57.79% to 26.17%. In conclusion, the knockout expression of multiple FAD2 genes increased the soybean oleic acid content and decreased the linoleic acid content in different receptors.

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Section: Discussioncontrasting

confidence: 64%

Construction and Functional Evaluation of CRISPR/Cas9 Multiple Knockout Vectors of the FAD2 Gene Family

Zhang

Xiao

et al. 2023

Agronomy

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“…These platforms offer enhanced design flexibility and make it easier to manufacture ZFNs that are customized to a variety of target sites in horticultural crops. ZFN improvements have mostly focused on improving specificity, which has led to a reduction in off-target effects, as well as an improvement in precision and safety in ZFNmediated genome editing in horticultural crops [79].…”

Section: Zfns (Zinc Finger Nucleases)mentioning

confidence: 99%

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Bhavanee,

Krishnamoorthi,

Rathva

et al. 2024

JABB

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Genetic engineering has emerged as a powerful tool in the field of horticulture to enhance the traits of crops, leading to improved yield, quality, and resistance to biotic and abiotic stresses. This comprehensive review explores the recent advancements in genetic engineering techniques applied to horticulture crops, providing a detailed overview of the innovative strategies employed to manipulate plant genomes. The review begins by discussing the evolution of genetic engineering in horticulture, highlighting key milestones and breakthroughs that have paved the way for current advancements. It covers a range of techniques such as CRISPR-Cas9, RNA interference, and synthetic biology, shedding light on their applications in modifying specific genes responsible for desired traits. The main focus of the review is on the enhancement of key horticultural traits, including disease resistance, pest resistance, abiotic stress tolerance, and post-harvest attributes. Examples from various horticultural crops, such as fruits, vegetables, and ornamental plants, illustrate the success stories and potential applications of genetic engineering in each category. The ethical and regulatory aspects of genetic engineering in horticulture are also explored, addressing concerns related to environmental impact, biodiversity, and consumer acceptance. The review emphasizes the importance of responsible and sustainable practices in the application of genetic engineering to ensure the long-term benefits without adverse consequences. Furthermore, the review delves into emerging trends and future prospects in the field, including the potential of genome editing for precision breeding, the use of omics technologies for targeted trait improvements, and the integration of genetic engineering with other breeding techniques. It also discusses the challenges and opportunities associated with the global adoption of genetically modified horticulture crops.

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“…In a study, FAD2-1A and FAD2-1B genes were targeted in soybeans, which play a role in the conversion of oleic acid to linoleic acid, impacting oil composition and nutritional quality [79]. Through CRISPR-Cas9-mediated small insertions or deletions in these genes, they successfully enhanced the oleic acid content, thereby reducing levels of saturated fats and improving the nutritional quality of soybean oil [80].…”

Section: Genome Editing For Quality Improvementmentioning

confidence: 99%

Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

Daniel¹,

Raveendar

et al. 2023

Horticulturae

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Genome editing has emerged as a powerful tool for accelerating crop improvement in horticultural crops by enabling precise modifications to their genetic makeup. This review provides an in-depth exploration of the applications, methodologies, and potential impacts of genome editing in horticulture. The review focuses on three major genome editing tools in horticulture, CRISPR-Cas9, TALENs, and ZFNs. The underlying mechanisms, applications, and potential challenges associated with each tool are discussed in detail. CRISPR-Cas9, being a versatile and widely used system, has the potential to enhance traits such as disease resistance, abiotic stress tolerance, nutritional content, and yield in horticultural crops. TALENs and ZFNs, although less commonly used, offer alternative options for targeted DNA modifications, and have demonstrated success in specific applications. We emphasize the potential benefits of genome editing in horticulture, including improved crop productivity, quality, and nutritional value. However, challenges such as off-target effects, delivery methods, and regulatory frameworks need to be addressed for the full realization of this technology’s potential. This review serves as a valuable resource for researchers, policymakers, and stakeholders, providing insights into the opportunities and complexities associated with harnessing genome editing for enhanced traits in horticultural crops. By navigating these challenges, genome editing can contribute to sustainable advancements in horticulture, benefiting both producers and consumers worldwide.

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Novel Allele of FAD2-1A from an EMS-Induced Mutant Soybean Line (PE529) Produces Elevated Levels of Oleic Acid in Soybean Oil

Cited by 6 publications

References 38 publications

Construction and Functional Evaluation of CRISPR/Cas9 Multiple Knockout Vectors of the FAD2 Gene Family

Construction and Functional Evaluation of CRISPR/Cas9 Multiple Knockout Vectors of the FAD2 Gene Family

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

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