Novel amine-functionalized iron trimesates with enhanced peroxidase-like activity and their applications for the fluorescent assay of choline and acetylcholine

Valekar, Anil H.; Batule, Bhagwan S.; Kim, Moon Il; Cho, Kyung‐Ho; Hong, Do‐Young; Lee, U‐Hwang; Chang, Jong‐San; Park, Hyun Gyu; Hwang, Young Kyu

doi:10.1016/j.bios.2017.08.056

Cited by 104 publications

(31 citation statements)

References 61 publications

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“…The solvent accessible pore volume comprises 43.7% of its unit cell volume as calculated by Platon. The overall double-layered geometry of complex 1 was similar to a previously reported structure that was constructed from TCPP linker and sodium ion, which represents a seldom seen architecture in MPFs ( 7 , 8 ). Moreover, there are the N-H…N and C-H…O hydrogen bonds in the packing structure.…”

Section: Resultssupporting

confidence: 77%

“…Metal-organic frameworks (MOFs) are a class of porous crystalline materials that are constructed from judiciously designed multi-dentate organic linkers and discrete metal ions/clusters ( 1 , 2 ). Due to their structural diversity and tunability as well as their promising applications in gas storage/separation ( 3 , 4 ), chemical sensing ( 5 ), heterogeneous catalysis ( 6 , 7 ), and biomedical usage ( 8 ), etc., MOFs have attracted tremendous research interest in the past two decades. Among the vast family of organic ligands used in constructing MOFs, porphyrins are a family of biologically relevant tetrapyrrole macrocycles ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]

2018

Braz J Med Biol Res

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A novel heterometallic metal-porphyrinic framework (MPFs) built from Y and K ions as nods and meso-tetra(4-carboxyphenyl)porphyrin as linkers has been successfully synthesized and characterized. The single crystal X-ray diffraction indicated that this complex 1 exhibited a bilayered architecture of the porphyrins, which is seldom seen in MPFs. In addition, in vitro anticancer activity of complex 1 on three human breast cancer cells (BT474, SKBr-3 and ZR-75-30) was further determined.

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Section: Resultssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]

2018

Braz J Med Biol Res

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“…This reaction produces free ·OH radicals that interact with AUR to create oxidized AUR. Based on previous reports, the concentration of ACh in body fluid is extremely low, generally in the nanomolar range [ 34 , 35 ]. Thus, AUR was exploited instead of other peroxidase substrates to monitor the presence of ACh since it can produce a very sensitive, highly fluorescent product (AURox).…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

2021

Self Cite

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Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

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“…Since the peroxidase-like activity of CoAC to oxidize employed substrate is promoted by H 2 O 2 , the ACs were then employed to detect H 2 O 2 dissolved in sample solution. To achieve higher sensitivity, another peroxidase-mediated substrate, AUR, which is also widely used for the peroxidase-mediated reaction at around neutral pH condition, was employed in this assay system [ 28 ]. Through the typical reaction condition described in Experimental section, fluorescence intensity by the oxidation of AUR was specifically observed with both CoAC and H 2 O 2 while other control cases exhibited negligible fluorescence intensities, indicating that CoAC displayed high peroxidase activity with AUR as a substrate ( Figure 5 a).…”

Section: Resultsmentioning

confidence: 99%

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

Song

Lee

Bui

et al. 2018

Sensors

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In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol–gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex® UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 μM to 1 mM with a lower detection limit of 5 μM. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules.

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Novel amine-functionalized iron trimesates with enhanced peroxidase-like activity and their applications for the fluorescent assay of choline and acetylcholine

Cited by 104 publications

References 61 publications

Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]

Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]

Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

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