Novel and simple method for isolating autologous mesothelial cells from the tunica vaginalis

Asano, Touko; Takazawa, Ryoji; Yamato, Masayuki; Kageyama, Yukio; Kihara, Kazunori; Okano, Teruo

doi:10.1111/j.1464-410x.2005.05816.x

Cited by 19 publications

(16 citation statements)

References 14 publications

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“…11 Recently, we proposed a new technique for harvesting autologous mesothelial cells from tunica vaginalis without intra-abdominal maneuvers. 16 In the current study, we confirmed the efficacy of autologous mesothelial cell sheet transplantation for preventing post-operative adhesions in a canine adhesion model. Fibrinolytic activity gradually increases as mesothelial cells expand, and it becomes greatest when re-peritonealization is complete.…”

Section: Discussionsupporting

confidence: 78%

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Transplantation of an Autologous Mesothelial Cell Sheet Prepared from Tunica Vaginalis Prevents Post-Operative Adhesions in a Canine Model

et al. 2006

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Post-operative adhesions often cause severe complications such as bowel obstruction and abdominopelvic pain. Previously, we reported that transplantation of a mesothelial cell sheet is effective for preventing adhesion in rat model. We also proposed a new technique for harvesting autologous mesothelial cells from tunica vaginalis without intra-abdominal maneuvers. In this study, we examined whether an autologous mesothelial cell sheet can prevent post-operative peritoneal adhesions in a canine adhesion model. Mesothelial cells were isolated from the tunica vaginalis of male beagles. Isolated cells were cultured on fibrin gel. We named this construct the "mesothelial cell sheet." Animals underwent surgery to induce peritoneal adhesion formation and were then transplanted with the mesothelial cell sheets (sheet group, n = 4), fibrin gel (fibrin group, n = 4), or no materials (sham group, n = 4). Four weeks after the transplantation, we evaluated adhesion formation and scored adhesion levels. The abdominal wall transplanted with the mesothelial cell sheet was covered with mesothelium. The total adhesion score of the sheet group was significantly lower than that of the fibrin group and the sham group. These results indicated that transplantation of an autologous mesothelial cell sheet is effective for preventing post-operative adhesion formation in the canine adhesion model. Our mesothelial cell sheet has the potential to be a powerful adhesion prophylactic material in surgery.

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Section: Discussionsupporting

confidence: 78%

“…16 Embryologically, the tunica vaginalis originates from the peritoneum and descends along with the testes into the scrotum during fetal development. 17 Therefore, the tunica vaginalis is a useful source of mesothelial cells because surgical isolation is convenient and free from abdominal complications.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of an Autologous Mesothelial Cell Sheet Prepared from Tunica Vaginalis Prevents Post-Operative Adhesions in a Canine Model

et al. 2006

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“…Takazawa et al and Asano et al. seeded a gel‐like fibrin matrix with mesothelial cells that had been isolated and cultured, and came up with the idea of transplanting them in sheets .…”

Section: Attempts To Apply Cell Sheet Technology To Peritoneal Regenementioning

confidence: 99%

Therapeutic Applications of Mesothelial Cell Sheets

et al. 2014

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Mesothelial cells are an integral part of the peritoneum and play an important role in maintaining its structural and functional properties. In recent years a number of studies on mesothelial cells have been performed to evaluate the localization, secretional properties and the ability of regeneration and transdifferentiation of these cells. They are also involved in the repair of the peritoneum damage following surgery or peritonitis. Mesothelial cells produce several cytokines, growth factors and extracellular matrix components, possessing anti-inflammatory and immunomodulatory properties. Based on previous research, cell sheet engineering has made it possible to transplant cells that retain the cells' function, and stacking different cells in layers has also become possible. Arranging blood vessels between the cell layers is a problem when stacking cells in layers. Whether adequate blood flow can be obtained for the cell layers to survive long-term is the difference between success and failure. Mesothelial cell transplantation for peritoneal regeneration needs to be performed under conditions in which the surface area of the visceral peritoneum is large and the mesothelial cell damage area is small. In this article we explain cell sheet engineering as one of the technologies for transplanting cells with a variety of intercellular adhesion and cell membrane molecules maintained intact, and its application to peritoneal regeneration.

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“…Several adhesion prevention strategies have been proposed, including the inhibition of inflammation (Avsar et al ., ), prevention of fibrin formation and promotion of fibrinolysis (Topal et al ., ), anti‐angiogenesis (Chiang et al ., ; Greene et al ., ) and tissue separation using hyaluronan‐based membranes (Becker et al ., ). Recently, a tissue engineering approach was suggested to promote regeneration of the peritoneum and mesothelium (Asano et al ., ; Takazawa et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model

Kawanishi

Yamato

Sakiyama

et al. 2013

J Tissue Eng Regen Med

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Postoperative intra-abdominal adhesions remain an unsolved problem despite significant progress in the surgical procedures themselves. They often lead to small-bowel obstruction, chronic abdominal and pelvic pain, as well as female infertility. The loss of mesothelial cells and several components of the inflammatory system following injury to the peritoneum results in fibrin formation and angiogenesis. The remaining fibrin matrix and angiogenesis lead to replacement by fibroblasts and fibrous band formation. The aim of this study was to develop a new therapeutic method of preventing intra-abdominal adhesions. We fabricated transplantable peritoneal cell sheets from the rat peritoneum by cell sheet engineering using a temperature-responsive culture system. The peritoneal cell sheets developed were composed of an upper monolayer of mesothelial cells and underlying multilayered fibroblasts, similar to the peritoneum in vivo. Transplantation of peritoneal cell sheets prevented tissue adhesion, fibrin deposition and angiogenesis, and, moreover, lymphangiogenesis and macrophage infiltration in a rat caecum cauterization adhesion model. Copyright © 2013 John Wiley & Sons, Ltd.

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Novel and simple method for isolating autologous mesothelial cells from the tunica vaginalis

Cited by 19 publications

References 14 publications

Transplantation of an Autologous Mesothelial Cell Sheet Prepared from Tunica Vaginalis Prevents Post-Operative Adhesions in a Canine Model

Transplantation of an Autologous Mesothelial Cell Sheet Prepared from Tunica Vaginalis Prevents Post-Operative Adhesions in a Canine Model

Therapeutic Applications of Mesothelial Cell Sheets

Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model

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