Novel angiotensin I-converting enzyme inhibitory peptides from protease hydrolysates of Qula casein: Quantitative structure-activity relationship modeling and molecular docking study

Lin, Kai; Zhang, Lanwei; Han, Xue; Cheng, Dansong

doi:10.1016/j.jff.2017.03.008

Cited by 95 publications

(67 citation statements)

References 58 publications

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“…Finally, molecular docking simulations have also been developed to predict possible interactions of peptides with proteins (e.g., active sites of enzymes), which are the target of the biological activity (Li-Chan, 2015). Examples in the literature include studies on ACE as well as DPP-IV inhibitory peptides (Lin et al, 2017;Nongonierma et al 2014). Thus, in silico tools may expedite the discovery and production of bioactive peptides from food proteins, although they still have some limitations (e.g., do not consider secondary, tertiary and quaternary structures of proteins to predict cleavage sites; and assume enzymes with stringent substrate specificity, which is not always the case in food applications where complex proteaseproteins interactions occur) (Li-Chan, 2015;Nongonierma and FitzGerald, 2016a;Udenigwe, 2014).…”

Section: Removing Bitternessmentioning

confidence: 99%

Peptides: Production, bioactivity, functionality, and applications

Hajfathalian

Ghelichi

Moreno

et al. 2017

Critical Reviews in Food Science and Nutrition

138

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Production of peptides with various effects from proteins of different sources continues to receive academic attention. Researchers of different disciplines are putting increasing efforts to produce bioactive and functional peptides from different sources such as plants, animals, and food industry by-products. The aim of this review is to introduce production methods of hydrolysates and peptides and provide a comprehensive overview of their bioactivity in terms of their effects on immune, cardiovascular, nervous, and gastrointestinal systems. Moreover, functional and antioxidant properties of hydrolysates and isolated peptides are reviewed. Finally, industrial and commercial applications of bioactive peptides including their use in nutrition and production of pharmaceuticals and nutraceuticals are discussed.

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Section: Removing Bitternessmentioning

confidence: 99%

Peptides: Production, bioactivity, functionality, and applications

Hajfathalian

Ghelichi

Moreno

et al. 2017

Critical Reviews in Food Science and Nutrition

138

View full text Add to dashboard Cite

show abstract

“…Tri‐peptides were chosen as research object in the present studies, as to a peptide with smaller molecular weight is likely to cross cell membrane and intestinal barrier (Hu, Chi, Wang, & Deng, ). At the same time, it has been documented that short peptide interacts well with ACE active sites (Lin et al, ). Additionally, many studies have revealed that tri‐peptides with reasonable molecular weight show significant ACE inhibitory activity, such as MCS (Jia, Wu, Yan, & Gui, ), VWP, and VNP (Chen et al, ).…”

Section: Introductionmentioning

confidence: 96%

“…Therefore, synthetic ACE inhibitory medicines (Captopril, Enalapril, and Lisinopril et al) arose at the historic moment. However, these medicines result in many side effects, such as headache, chest pain, tachycardia, skin rashes, chest pain, and taste disturbances (Lin, Zhang, Han, & Cheng, ). Herein, the research about natural ACE inhibitors without side effects became an important field, especially the food‐derived ACE inhibitory peptides (Lee & Hur, ).…”

Section: Introductionmentioning

confidence: 99%

Novel ACE inhibitors derived from soybean proteins using in silico and in vitro studies

Zhao

Xue

et al. 2019

J Food Biochem

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The widespread application of soybean‐derived peptides is currently limited due to the challenges in the identification of peptides. In the present work, in silico and in vitro analysis were applied to identify ACE inhibitory tri‐peptides from soybean protein. The soybean protein was cleaved by PeptideCutter. Then, unknown tri‐peptides were selected to solubility estimation and ADME prediction. Subsequently, Discovery Studio was applied to evaluate the interaction mechanism between ACE and tri‐peptides. Finally, in vitro activity of theoretical ACE inhibitory tri‐peptides was verified by RP‐HPLC method. As a result, DMG was selected as a potent ACE inhibitory peptide. Cell experiment showed that DMG had no cytotoxic effects on HEK‐293 cells. And molecular docking results indicated that DMG contacted well with ACE’s active sites (Gln281, His353, Ala354, Glu384, Lys511, His513, and Tyr520). Furthermore, DMG could exert potent activity against ACE, with IC50 value of 3.95 ± 0.11 mM. Practical applications Present research showed soybean is a potential protein resource to obtain ACE inhibitory peptides. Simultaneously, virtual screening method is a feasible way to substitute for classical method in emerging nutritional fields. What's more, present study provides a theoretical basis for industrial research on foodstuff for ACE inhibitory peptides without side effects.

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“…). Many studies on transport through the Caco‐2 cell monolayers system have indicated that oligopeptides, especially those larger than three amino acid residues, were usually cleaved into shorter peptides before their absorption . However, some of them still can be transported intact through the Caco‐2 cell monolayers …”

Section: Resultsmentioning

confidence: 99%

“…Many studies on transport through the Caco-2 cell monolayers system have indicated that oligopeptides, especially those larger than three amino acid residues, were usually cleaved into shorter peptides before their absorption. 21,31,32 However, some of them still can be transported intact through the Caco-2 cell monolayers. 2,22 PepT1, paracellular route and transcytosis are three probable mechanisms for small molecular substances (i.e., drugs or peptides) transport across the intestinal epithelial membrane.…”

Section: Stability Of Gp-12 During In Vitro Digestion and Absorptionmentioning

confidence: 99%

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Zhang

Zhu

et al. 2020

J Sci Food Agric

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BACKGROUND As the world's population is transitioning gradually to an ageing stage, the incidence of osteoporosis is increasing annually. Yak bone is one of the major components of Tibetan medicine and it has mainly been associated with an improvement in bone health, for example against osteoporosis. However, the functional bioactive ingredients and the underlying mechanisms are still unclear. RESULTS Sequential purification of yak‐bone hydrolysates was achieved by ultrafiltration, size exclusion chromatography, and semi‐preparative reverse‐phase high‐performance liquid chromatography. After this, 35 novel peptides were identified by mass spectrometry analysis, of which peptide GPAGPPGPIGNV (GP‐12) displayed the highest osteoblast proliferation‐promoting activity, with an increase of 42.7% in cell growth. An in vitro stability study demonstrated that GP‐12 was digested into smaller peptides (GP‐9, GV‐9, AV‐10 and GP‐11) after simulated gastrointestinal digestion and absorption (Caco‐2 cell monolayers) experiments. However, some of them still can be absorbed intact through the (Caco‐2 cell monolayers by a paracellular route (Papp: 5.36 ± 0.34 cm s−1). Flow cytometry results indicated that GP‐12 enhanced osteoblastic proliferation by inducing the alteration of the cell‐cycle progression both from the G0/G1 to the S phase and from the S to the G2/M phase. Quantitative real‐time polymerase chain reaction (PCR) and western blot results revealed that GP‐12 induced osteoblastic proliferation and differentiation in a dose–response manner through the activation of a Wnt/β‐catenin signaling pathway. CONCLUSION These findings highlighted that such peptides hold the promise of discovering candidates for functional and health‐promoting foods, which could be potentially used for the treatment of osteoporosis. © 2020 Society of Chemical Industry

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Novel angiotensin I-converting enzyme inhibitory peptides from protease hydrolysates of Qula casein: Quantitative structure-activity relationship modeling and molecular docking study

Cited by 95 publications

References 58 publications

Peptides: Production, bioactivity, functionality, and applications

Peptides: Production, bioactivity, functionality, and applications

Novel ACE inhibitors derived from soybean proteins using in silico and in vitro studies

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

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