Novel anionic surfactant-modified chlorhexidine and its potent antimicrobial properties

Muneeswaran, Zilma Pereira; Teoman, Baran; Wang, Yu; Chaudhry, Haroon; Brinzari, Tatiana V.; Verma, Gaurav; Ranasinghe, Lomaani; Kaler, Kylie Ryan; Huang, Xiaoyi; He, Xing; Thomas, Belvin; Xu, Shiyou; Cheng, Chi-Yuan; Boyd, Jeffrey M.; Chen, Dailin; Hao, Zhigang; Ma, Shengqian; Asefa, Tewodros; Pan, Long; Dubovoy, Viktor

doi:10.1039/d3dt02559d

Cited by 3 publications

(1 citation statement)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is crucial as it indicates the longlasting antimicrobial effects, which play a vital role in ensuring the sustained clinical success of dental restorative treatments. The long-term antibacterial effect of functional adhesives is attributed to the controlled release of CHX, which destroys the function of the bacterial cell through the coordination of guanidine groups in the bacterial phospholipid cell membrane, thus exerting its bactericidal activity (Muneeswaran et al, 2003). The acidic environment created by bacterial biofilms promotes increased release of CHX from functional adhesives, further enhancing its antibacterial effectivity over time.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial properties and bonding durability of pH-responsive dentin adhesives with chlorhexidine-load mesoporous silica nanoparticles

Li,

Qi,

Liu

2024

Front. Mater.

View full text Add to dashboard Cite

In this study, we synthesized pH-sensitive CHX@SBA-PDA nanoparticles and characterized their structure. These nanoparticles were then incorporated into Single Bond 2 commercial dentin adhesive. Subsequently, timely and long-term antibacterial evaluation, cytotoxicity evaluation and bonding properties were conducted. The results demonstrated the successful synthesis of CHX@SBA-PDA nanoparticles. Moreover, CHX@SBA-PDA exhibited excellent pH sensitivity and maintained a high release rate of chlorhexidine (CHX) under cariogenic low pH conditions. At pH 5.0, the release rate could reach up to 71.1% after 24 h of incubation with CHX@SBA-PDA nanoparticles. Among the different adhesive formulations tested, the 2% wt adhesive displayed the strongest immediate and 30 days bacterial inhibition ability (p < 0.05). No significant difference was observed in immediate shear strength among the four groups (p > 0.05). After undergoing pH cycling, all functional adhesive groups exhibited higher shear strength compared to the control group (p < 0.05). Furthermore, there was no significant difference in cell proliferation activity between the experimental group and control group (p > 0.05). In conclusion, our functional dentin adhesive containing CHX@SBA-PDA nanoparticles demonstrated long-term antibacterial properties as well as improved bond strength characteristics, thus, offering a promising approach to enhance durability of bonded restorations.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial properties and bonding durability of pH-responsive dentin adhesives with chlorhexidine-load mesoporous silica nanoparticles

Li,

Qi,

Liu

2024

Front. Mater.

View full text Add to dashboard Cite

show abstract

NMR of soft matter systems

Wallace

2024

Nuclear Magnetic Resonance

View full text Add to dashboard Cite

This chapter summarises recent advances and applications of solid-state, solution-state and gel-state NMR techniques to study soft matter systems. Original research articles published between March 2023 and March 2024 are discussed that cover surfactants, liquid crystals, polymer solutions, gels and other soft matter systems. Each section of the review focuses on a different NMR technique, including 1D and 2D solution-state experiments, 2H NMR, nuclear Overhauser effect (NOE) and saturation transfer difference (STD) experiments, pulsed-field gradient (diffusion) NMR, relaxation measurements, experiments on quadrupolar counterions and solid-state NMR.

show abstract

Potentiality of Antibacterial Gels for the Prophylactic Coating of Hernia Repair Prosthetic Materials

Pérez-Köhler,

Benito-Martínez,

Rivas-Santos

et al. 2024

Gels

View full text Add to dashboard Cite

Prosthetic mesh infection constitutes one of the major postsurgical complications following abdominal hernia repair. Antibacterial coatings represent a prophylactic strategy to reduce the risk of infection. This study assessed the in vitro response of two antibacterial gels made of 1% carboxymethylcellulose (CMC) functionalized with an antiseptic (chlorhexidine, CHX) or an antibiotic (rifampicin, RIF), developed for the coating of polypropylene (PP) meshes for hernia repair. Fragments of a lightweight PP mesh (1 cm2) presoaked in the unloaded or drug-loaded CMC (0.05% CHX; 0.13 mg/mL RIF) were challenged with 106 CFU/mL Staphylococcus aureus (Sa) and methicillin-resistant S. aureus (MRSA). Agar diffusion tests, sonication, turbidimetry, crystal violet staining, scanning electron microscopy and cell viability assays (fibroblasts, mesothelial cells) were performed to evaluate the response of the gels. Both compounds—especially the RIF-loaded gel—exerted a biocidal effect against gram-positive bacteria, developing wide inhibition halos, precluding adhesion to the mesh surface, and hampering bacterial survival in culture. The antibiotic gel proved innocuous, while lower viability was found in cells exposed to the antiseptic (p < 0.05). Together with their fast, affordable, convenient processing and easy application, the results suggest the potential effectiveness of these drug-loaded CMC gels in providing meshes with an antibacterial coating exhibiting great biocide performance.

show abstract

Novel anionic surfactant-modified chlorhexidine and its potent antimicrobial properties

Abstract: Chlorhexidine dodecyl sulfate (CHX-DS) was synthesized and characterized via single-crystal X-ray diffraction (SC-XRD), 1H nuclear magnetic resonance (NMR), 1H nuclear Overhauser effect spectroscopy (NOESY), and attenuated total reflectance Fourier-transform infrared...

Cited by 3 publications

References 49 publications

Antimicrobial properties and bonding durability of pH-responsive dentin adhesives with chlorhexidine-load mesoporous silica nanoparticles

Antimicrobial properties and bonding durability of pH-responsive dentin adhesives with chlorhexidine-load mesoporous silica nanoparticles

NMR of soft matter systems

Potentiality of Antibacterial Gels for the Prophylactic Coating of Hernia Repair Prosthetic Materials

Contact Info

Product

Resources

About