Novel antibacterial nanofibrous PLLA scaffolds

Abstract

In order to achieve high local bioactivity and low systemic side effects of antibiotics in the treatment of dental, periodontal and bone infections, a localized and temporally controlled delivery system is crucial. In this study, a three-dimensional (3D) porous tissue engineering scaffold was developed with the ability to release antibiotics in a controlled fashion for long-term inhibition of bacterial growth. The highly soluble antibiotic drug, Doxycycline (DOXY), was successfully incorporated into PLGA nanospheres using a modified water-in-oil-in-oil (w/o/o) emulsion method. The PLGA nanospheres (NS) were then incorporated into prefabricated nanofibrous PLLA scaffolds with a well interconnected macroporous structure. The release kinetics of DOXY from four different PLGA NS formulations on a PLLA scaffold was investigated. DOXY could be released from the NS-scaffolds in a locally and temporally controlled manner. The DOXY release is controlled by DOXY diffusion out of the NS and is strongly dependent upon the physical and chemical properties of the PLGA. While PLGA50-6.5K, PLGA50-64K, and PLGA75-113K NSscaffolds discharge DOXY rapidly with a high initial burst release, PLGA85-142K NS-scaffold can extend the release of DOXY to longer than 6 weeks with a low initial burst release. Compared to NS alone, the NS incorporated on a 3-D scaffold had significantly reduced the initial burst release. In vitro antibacterial tests of PLGA85 NS-scaffold demonstrated its ability to inhibit common bacterial growth (S.aureus and E.coli) for a prolonged duration. The successful incorporation of DOXY onto 3-D scaffolds and its controlled release from scaffolds extends the usage of nano-fibrous scaffolds from the delivery of large molecules such as growth factors to the delivery of small hydrophilic drugs, allowing for a broader application and a more complex tissue engineering strategy.

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

“…Infection is caused by either bacterial colonization at the wound site or foreign body response resulting from the implant material [5].Antibacterial biomaterials are one of the greatest interests in the war against implant-related infections, representing the broadest group of anti-infective biomaterials [6]. Focal antibacterial drug-loaded biomaterials can deliver a desired drug dose directly to the infected site for an extended time period while minimizing systemic distribution of toxic drugs [7,8]. Infection associated with GTR/GBR implant is mainly caused by anaerobic bacteria [9,10].…”

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

“…Feng et al 65 reported a three-dimensional (3D) porous tissue engineering scaffold, which was able to release antibiotics in a controlled fashion for long-term inhibition of bacterial growth. A highly soluble antibiotic, doxycycline (DOXY), was incorporated into PLGA nanospheres (NSs) using a water-in-oil-in-oil (w/o/o) emulsion method.…”

“…The successful incorporation of DOXY onto 3D scaffolds and its controlled release from scaffolds suggests that the usage of nanofibrous scaffolds in the delivery of large molecules (such as growth factors) to the delivery of small hydrophilic drugs allows for a broader application and a more complex tissue engineering strategy. 65 Similarly, PLGA was employed to compose a poly (ethylene glycol) monomethyl ether (mPEG) and PLGA copolymer as a sol-gel drug delivery system for treating osteomyelitis. 66 This delivery system was shown to have several advantages in treating osteomyelitis, including easy preparation, 100% encapsulation rate, near-linear sustained release of drugs, injectable design, and in situ gelling of the target tissue.…”

Peri̇odontal Doku Mühendi̇sli̇ği̇nde Kullanilan İskele Yapilari

“…43 Üç boyutlu nanoküreli PLLA iskelenin, bakteriyal gelişimin inhibisyonu için doksisilinin kontrollü olarak salınım kabiliyetini değerlendiren bir çalışmada, makroporöz ve nanofibröz yapıların iyi derecede birleştiği, 6 haftadan daha uzun zamanda bakteriyel gelişimi baskıladığı gösterilmiş ve böylece nanoküre iskele sistemlerinin protein ve ilaç moleküllerinin taşınması ile doku mühendisliği uygulamalarında kullanılabileceği bildirilmiştir. 44 …”