Novel antifungal antibiotics maniwamycins A and B. I. Taxonomy of the producing organism, fermentation, isolation, physico-chemical properties and biological properties.

Abstract

Twoantifungal antibiotics maniwamycins A and B were isolated from the culture broth of a strain of actinomycetes, which were classified as Streptomyces prasinopilosus. These antibiotics were isolated by resin absorption and extraction with EtOAcand purified by column chromatography. Both antibiotics were found to be new azoxy substances from their physico-chemical properties. They showed broad antifungal spectra.In the course of screening for new antifungal agents from culture broths, wehave discovered the novel antifungal antibiotics, maniwamycins A and B^produced by a Streptomyces sp. This paper details the taxonomy of the producing organism, isolation, and physico-chemical and biological properties oi these antibiotics. The structural elucidation of antibiotics maniwamycinsA and B are described in ar accompanying paper. 1}

Jietacins with potent nematocidal activity; efficient isolation of novel analogs and divergent total synthesis of jietacin A, B, C, and D

“…The comparison of 1 H, 13 C NMR, and IR between natural product and synthetic sample is shown in Supplementary data. 4.2.12.…”

“…The comparison of 1 H, 13 C NMR, and IR between the natural product and the synthetic sample is shown in Supplementary data. The data of 1 H, 13 C NMR, and IR was identical to the authentic sample.…”

“…The data of 1 H, 13 C NMR, and IR was identical to the authentic sample. The comparison of 1 H, 13 C NMR, and IR between the natural product and the synthetic sample is shown in Supplementary data.…”

“…1), contained a vinylazoxy group with an aliphatic long chain, and displayed potent nematocidal activity. 11 Although there are some natural products, such as valanimycin (3), 12 maniwamycins (4), 13 azoxybaciline (5), 14 and elaiomycin (6), 15 which possess an azoxy group, natural products with a vinylazoxy group have rarely been reported (Fig. 1).…”

“…1 H NMR and NOE spectra were recorded at 600 MHz Agilent Technologies INOVA 600 (600 MHz). The chemical shifts are expressed in ppm downfield from internal solvent peaks CDCl 3 (7.26 ppm, 1 H NMR), CDCl 3 (77.00 ppm,13 C NMR), and coupling constant (J values) are given in Hertz. The coupling patterns are expressed by s (singlet), d (doublet), dd (double doublet), dt (double triplet), t (triplet), tt (triple triplet), q (quartet), m (multiplet), br (broadened).…”