Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis.

Itō, Akira; Plancarte, A.; Ma, Liang; Kong, Yoon; Flisser, Ana; Cho, Seung Yull; Liu, Yuehan; Kamhawi, S.; Lightowlers, Marshall W.; Schantz, Peter

doi:10.4269/ajtmh.1998.59.291

Cited by 116 publications

(113 citation statements)

References 15 publications

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“…Further diagnosis could be achieved by coproscopy, i.e., demonstrating eggs of the parasite in stool samples [52,54]. For the differential diagnosis of cysticercosis, a highly specific western blot based on Taenia solium-derived glycoproteins and an ELISA based on recombinant antigens are available [55,56], although in EITB cross reactivity with sera from AE patients with non-diagnostic higher molecular weight components have been reported [57]. Schistosomosis in imaging studies mainly appears in the periportal tissue together with fibrosis, calcification (mainly in Schistosoma japonicum, rare in S. mansoni) and thickening of the walls of periportal septae and the liver capsule [58], features usually not seen in Echinococcus patients.…”

Section: Discussionmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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Section: Discussionmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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“…Enzyme linked immunosorbent assay (ELISA) and immunoblot were carried out using glycoproteins (GPs) from a pH 8.1 fraction purified by preparative isoelectric focusing from T. solium cysts (Rotofor, BioRad, USA) (Ito et al, 1998(Ito et al, , 1999) and a chimeric recombinant antigen (modified from Sako et al, 2000). Detection of antibody by ELISA was performed as described by Ito et al (1998Ito et al ( , 1999 and Sako et al (2000).…”

Section: Elisa Immunoblot and Copro-elisamentioning

confidence: 99%

“…(Allan et al, 1992;Ito et al, 1998Ito et al, , 1999Nakao et al, 2002;Yamasaki et al, 2004aYamasaki et al, , 2004b. Cysticercosis cases were suspected by questionnaires on the history of epileptic seizures, detection of subcutaneous nodule by palpation and serological examination for T. solium cysticercosis .…”

Section: Introductionmentioning

confidence: 99%

High prevalence of Taenia saginata taeniasis and status of Taenia solium cysticercosis in Bali, Indonesia, 2002–2004

Wandra

Sutisna

Dharmawan

et al. 2006

Transactions of the Royal Society of Tropical Medicine and Hygi

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1 SummaryAn epidemiological survey of taeniasis/cysticercosis was carried out in one semi-urban and two urban villages in three districts in Bali, Indonesia in 2002 and. A total of 398 local people from 247 families were diagnosed by anamnesis and clinical examinations, and 60 residents were suspected to be taeniasis carriers. Among 60 suspected carriers, 56 persons expelled a total of 61 taeniid adult worms after praziquantel treatment. From 398 residents, 252 stool samples were available for analysis of taeniid eggs, copro-antigens or copro-DNA for identification of taeniid species, and 311 serum samples were available for detection of antibodies against Taenia solium cysticercosis. Taeniasis prevalences were highly variable among three villages (1.1-27.5%) and only one cysticercosis due to T. solium infection was detected. All expelled tapeworms were confirmed to be Taenia saginata by mitochondrial DNA analysis. There was no Taenia asiatica human case in Bali. Case control analysis of 106 families chosen at random from 179 families in 2004 and another 106 families from non-endemic areas revealed that risk factors of T. saginata taeniasis for families were the level of education (p < 0.01), the consumption of beef lawar (p < 0.01) and the source of lawar (p < 0.01).

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“…6 Thus, the disease may result primarily from the host inflammatory response to dying parasites. 7 Many efforts have been made to use homologous antigens, [8][9][10][11][12][13][14] T. crassiceps antigens and synthetic peptides, [15][16][17] a phage display peptide library, 18 and recombinant T. solium antigens in the immunodiagnosis of cysticercosis. [19][20][21][22] Host-parasite interaction is poorly understood in NCC.…”

Section: Introductionmentioning

confidence: 99%

Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

Molinari¹,

García-Mendoza²,

Garza³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. To detect IgG antibodies to Taenia solium, a controlled double-blind study was conducted using 91 coded cerebrospinal fluid samples from patients with neurocysticercosis (NCC) and other neurologic disorders. Samples were tested in an enzyme-linked immunosorbent assay (ELISA) using metacestode excretion/secretion antigens. The results were correlated with data from medical records on the diagnosis of NCC (based on computed tomography and magnetic resonance imaging criteria) and other neurologic disorders. The ELISA results were positive in 22 of the 24 cases with active NCC. In contrast, six cases with calcified cysts (inactive NCC), as well as one case in a transitional stage, were negative. One case with a calcified granuloma and another with a granuloma plus calcifications (classified as inactive NCC) had positive results. The remaining negative results corresponded to other neurologic disorders (58 cases). The results of the ELISA showed a significant difference between active and inactive NCC (P ‫ס‬ 0.0034).

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Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis.

Cited by 116 publications

References 15 publications

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

High prevalence of Taenia saginata taeniasis and status of Taenia solium cysticercosis in Bali, Indonesia, 2002–2004

Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

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