Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin

Abstract

Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.

Amaranth Protein Hydrolysates Efficiently Reduce Systolic Blood Pressure in Spontaneously Hypertensive Rats

“…On the contrary, when the hydrolysate was orally administered (by using feeding tubes), a significant effect was seen after 3 h. Similarly, Matsui et al [ 19 ] reported that an antihypertensive peptide and captopril could decrease the systolic blood pressure in spontaneous hypertensive rats after 3 h of oral administration (by using feeding tubes). Others have reported that the highest antihypertensive effect of the antihypertensive peptide GAAGGAF from Coix lachryma-jobi occurs after 4 h to 6 h of administration, and the effect remains until 7 h post-supplementation [ 20 ]. Our findings are in line with the previous studies and confirm that an optimized amaranth hydrolysate has a hypotensive effect in vivo.…”

Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin

“…ma-yuen Stapf ) Glutelin”. Change in Main Body Paragraphs There are three errors in this article [ 1 ]: Section 2.6. The ACE Inhibitory Activity of GAAGGAF In Vitro should be corrected to 2.6.…”

Impact of germination on the chemical components and bioactive properties of adlay (Coix lachryma‐jobi L.) water extract

“…Li et al . () had established that adlay glutelin hydrolysate can effectively inhibit ACE activity in vitro . The promoted ACE inhibitory activity might be attributed to the release of bioactive peptides during adlay germination.…”

“…A previous study also revealed that germination can improve the ACE inhibitory activity of legume samples (Mamilla & Mishra, 2017). Li et al (2017) had established that adlay glutelin hydrolysate can effectively inhibit ACE activity in vitro. The promoted ACE inhibitory activity might be attributed to the release of bioactive peptides during adlay germination.…”