Novel Antimicrobial Approaches to Combat Bacterial Biofilms Associated with Urinary Tract Infections

Mancuso, Giuseppe; Trinchera, Marilena; Midiri, Angelina; Zummo, Sebastiana; Vitale, Giulia; Biondo, Carmelo

doi:10.3390/antibiotics13020154

Cited by 3 publications

(2 citation statements)

References 129 publications

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“…Neurological disorders were the only comorbidities that occurred more frequently in the study group compared to the control group. The fact that patients in both groups had a high rate of urosepsis on admission, as in other studies [37], demonstrates the aggressiveness of MDR Kpn. However, mortality was lower, demonstrating the better prognosis of urosepsis of urinary origin.…”

Section: Discussionsupporting

confidence: 79%

“…Numerous studies have demonstrated bacterial colonization of catheters, including with Kpn through its ability to bind to biotic and abiotic surfaces via fimbriae [43]. After fixation, the germs form a bacterial biofilm [26,29,37,44], an element of bacterial persistence and the development of resistance to carbapenems [27]. We have shown that not only does the presence of Foley urethral catheters confer a risk of urinary tract infection with CR Kpn [6], but also the presence of double-J ureteral catheters, nephrostomy, cystostomy, or ureterostomy double-J catheters, as in other studies [26,27,29].…”

Section: Discussionmentioning

confidence: 99%

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Urinary Tract Infections with Carbapenem-Resistant Klebsiella pneumoniae in a Urology Clinic—A Case-Control Study

Radu,

Costache,

Onofrei

et al. 2024

Antibiotics

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Background: The aim of our study was to analyze the factors associated with the increased risk of urinary tract infection (UTI) with carbapenem-resistant (CR) Klebsiella pneumoniae (Kpn) and the antibiotic resistance spectrum of the strains in patients. As secondary objectives, we elaborated the profile of these patients and the incidence of different types of carbapenemases. Methods: We conducted a retrospective case-control study in which we compared a group of 62 patients with urinary tract infections with CR Kpn with a control group consisting of 136 patients with urinary tract infections with multidrug-resistant (MDR), but carbapenem-sensitive (CS), Kpn, who were hospitalized between 1 January 2022 and 31 March 2024. Results: Compared to patients with urinary tract infections with CS Kpn, patients with urinary tract infections with CR Kpn were preponderant in rural areas (62.9% vs. 47.1%, p = 0.038) and more frequently had an upper urinary tract infection (69.4% vs. 36.8%, p < 0.01). Among the risk factors examined, patients in the study group had a higher presence of urinary catheters inserted for up to one month (50% vs. 34.6%, p = 0.03), rate of hospitalization in the last 180 days (96.8% vs. 69.9%, p < 0.01) and incidence of antibiotic therapy in the last 180 days (100% vs. 64.7%, p < 0.01). They also had a higher rate of carbapenem treatment in the last 180 days (8.1% vs. 0%, p < 0.01). Patients in the study group had a broader spectrum of resistance to all antibiotics tested (p < 0.01), with the exception of sulfamethoxazole–trimethoprim, where the resistance rate was similar in both groups (80.6% vs. 67.6%, p = 0.059). In the multivariate analysis, transfer from other hospitals (OR = 3.51, 95% and CI: 1.430–8.629) and treatment with carbapenems in the last 180 days (OR = 11.779 and 95% CI: 1.274–108.952) were factors associated with an increased risk of disease compared to the control group. The presence of carbapenemases was observed in all patients with CR Kpn, in the order of frequency New Delhi metallo-ß-lactamase (NDM) (52.2%), Klebsiella pneumoniae carbapenemase (KPC) (32.6%), and carbapenem-hydrolyzing oxacillinase (Oxa-48) (15.2%). Conclusions: The environment of origin and previous treatment with carbapenems appear to be the factors associated with an increased risk of urinary tract infection with CR Kpn compared to patients with urinary tract infections with CS Kpn. CR Kpn exhibits a broad spectrum of antibiotic resistance, among which is resistance to carbapenem antibiotics.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Urinary Tract Infections with Carbapenem-Resistant Klebsiella pneumoniae in a Urology Clinic—A Case-Control Study

Radu,

Costache,

Onofrei

et al. 2024

Antibiotics

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Validation of the Russian version of the pelvic pain and urgency/frequency patient symptom scale (C.L. Parsons, 2000) for patients with chronic recurrent uncomplicated cystitis

Kupriyanov,

Zaitsev,

Bernikov

et al. 2024

Urology reports (St. - Petersburg)

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BACKGROUND: Questionnaires allow to objectify and quantify the disease severity, especially at the patient’s initial visit, its impact on the patients’ quality of life, as well as the dynamics of symptoms during treatment. AIM: To validate the Russian version of the Pelvic Pain and Urgency/Frequency Patient Symptom Scale (PUF scale; C.L. Parsons, 2000) for patients with chronic recurrent uncomplicated cystitis. MATERIALS AND METHODS: The Russian version of the PUF Scale was suggested to two respondent groups aged 19 to 53 years: relatively healthy volunteers, 15 women who did not complains of urinary disorders or pain/discomfort in the bladder, and 14 patients with verified diagnosis of chronic recurrent uncomplicated cystitis. RESULTS: The resulting data demonstrate the validity of the proposed Russian version of the PUF Scale. Statistically significant differences were noted in the answers to most of the questionnaire issues between two respondent groups: conditionally healthy volunteers and patients with chronic recurrent uncomplicated cystitis. A high level of internal consistency of the questionnaire was found. Cronbach’s alpha coefficient in the main group was 0.888, in the control group — 0.819. CONCLUSIONS: The study findings proved that the Russian version of the Pelvic Pain and Urgency/Frequency Patient Symptom Scale (C.L. Parsons, 2000) is a valid tool for subjective assessment of the severity of chronic recurrent uncomplicated cystitis symptoms.

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Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections

Purbowati,

Utami,

Raharjo

et al. 2024

J. Multidiscip. Appl. Nat. Sci.

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Uropathogenic Escherichia coli (UPEC) is responsible for 80–90% of urinary tract infections (UTI) in the global population. The emergence of the increasing resistance to broad-spectrum antimicrobial agents was due to the ability to form biofilms. Cell surface factors that play a role in biofilm formation include Quorum Sensing (QS) which is encoded by the luxS family gene and curli by two operons, namely the csgBA operon. The purpose of the study is to detect the effects of 2 virulence genes (csgD and luxS) on biofilm-forming UPEC associated with UTI. As many as 76 UPEC isolates were collected from the clinical microbiology laboratories and the biofilm development was analyzed using the crystal violet method on microplate 96 wells. Using PCR assay, the two studied genes (csgD and luxS) were determined to be present in the isolates. UPEC isolates the bacteria-produced biofilms (90.80%) and nonproducers (9.20%). Most UPEC bacteria (97.36%) are known to be positive for csgD and luxS gene, while the others (92.10%) are known to be positive for the luxS gene. The highest proportion of the genes expressed in this study is followed by the presence of a relationship between the ability to produce biofilm and the presence of the genes under investigation, which is followed by all UPEC strains that cause UTI in humans.

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Novel Antimicrobial Approaches to Combat Bacterial Biofilms Associated with Urinary Tract Infections

Cited by 3 publications

References 129 publications

Urinary Tract Infections with Carbapenem-Resistant Klebsiella pneumoniae in a Urology Clinic—A Case-Control Study

Urinary Tract Infections with Carbapenem-Resistant Klebsiella pneumoniae in a Urology Clinic—A Case-Control Study

Validation of the Russian version of the pelvic pain and urgency/frequency patient symptom scale (C.L. Parsons, 2000) for patients with chronic recurrent uncomplicated cystitis

Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections

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