Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

Suwandecha, Tan; Srichana, Teerapol; Balekar, Neelam; Nakpheng, Titpawan; Pangsomboon, Kanokporn

doi:10.1007/s00203-015-1126-z

Cited by 13 publications

(11 citation statements)

References 31 publications

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“…So far, scholars have confirmed the antibacterial effect of many natural and synthetic AMPs, including Bac8c, Aedesin, decapeptide, XLAsp-P1, LL-37 and variant, Pep-7, and HBD3-C15 [35,87,[110][111][112][113][114][115][116], which provided us with the study methods of this new AMP GERM CLEAN. What's more exciting, Wang et al [43,117] in our hospital successfully synthesized three new AMPs (GH8, GH12, and GH16) and further studied the bacteriostatic properties and mechanisms.…”

Section: Discussionmentioning

confidence: 96%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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Objective. To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on Streptococcus mutans (S. mutans) in vitro and further explore the related mechanisms at phenotypic and transcriptional levels. Methods. The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards S. mutans was determined by the broth dilution method. S. mutans virulence-related phenotypic assays including initial adhesive assay, pH drop, exopolysaccharides (EPS), and biofilm formation measurements and quantitative real-time PCR (qRT-PCR) were further applied to detect the inhibitory mechanisms of GREM CLEAN at 1/2MIC. Results. The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria S. mutans. The minimal inhibitory concentration of GERM CLEAN on S. mutans was 100% mass fraction (the stock solution). The study of the antibacterial mechanism showed that GERM CLEAN had a certain inhibitory effect on the initial adhesion, acid production, extracellular polysaccharides (EPS) production, and biofilm formation of S. mutans. GERM CLEAN disturbed S. mutans biofilm physiology mainly through destruction of biofilm architecture and suppression of bacterial growth. The results of qRT-PCR further confirmed that the expression levels of EPS and lactic acid generation genes including gtfB, gtfC, gtfD, and ldh were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results. Conclusion. The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti-Streptococcus mutans effect and the inhibitory property may be due to suppression of the virulence factors of S. mutans including adhesive, acidogenicity, EPS, and biofilm formation.

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Section: Discussionmentioning

confidence: 96%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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“…Furthermore, two studies have previously identified antibacterial peptides specifically targeting P . gingivalis (Franzman et al, ; Suwandecha, Srichana, Balekar, Nakpheng, & Pangsomboon, ). In addition to their therapeutic effect, target‐specific antibacterials may be used as a tool to study the role of a particular pathogen in complex microbial communities such as biofilms found in the oral cavity (Frias‐Lopez, ; Guo et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…As for the oral cavity, different studies have published promising results on antimicrobial peptides specifically designed to target S. mutans, a key pathogen involved in caries (Eckert et al, 2006;Guo et al, 2015;Mai et al, 2011). Furthermore, two studies have previously identified antibacterial peptides specifically targeting P. gingivalis (Franzman et al, 2009;Suwandecha, Srichana, Balekar, Nakpheng, & Pangsomboon, 2015).…”

Section: Discussionmentioning

confidence: 99%

Repurposing AM404 for the treatment of oral infections by Porphyromonas gingivalis

Gerits

Spincemaille

Cremer

et al. 2017

Clinical & Exp Dental Res

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Porphyromonas gingivalis is a major pathogen involved in oral diseases such as periodontitis and peri‐implantitis. Management of these diseases typically includes mechanical debridement of the colonized surfaces followed by application of antiseptics or antibiotics. Disadvantages associated with the use of antiseptics and the growing worldwide problem of antibiotic resistance have necessitated the search for alternative agents. In this study, the antibacterial and antibiofilm properties of AM404, an active metabolite of paracetamol, were tested against P. gingivalis and other bacterial pathogens. The activity of AM404 was tested against 10 bacteria, including both oral and nonoral human pathogens. The minimal inhibitory concentration (MIC) of AM404 was determined by measuring optical density (OD) values. The minimum biofilm inhibitory concentration (MBIC) was detected by crystal violet staining. The activity of structural analogs of AM404 was tested by MIC determinations. The effect of AM404 on P. gingivalis biofilms formed on titanium disks as a model for dental implants was evaluated by colony forming unit counting. Potential cytotoxicity of AM404 towards HEK‐293 (human embryonic kidney cells), HepG2 (human hepatoma cells), IEC‐6 (rat intestinal cells), and Panc‐1 cells (pancreatic cancer cells) was assessed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assays. To get more insight in the mode of action of AM404, we used the fluorescent dyes N‐phenyl‐1‐napthylamine and SYTOX green to investigate outer and inner membrane damage of P. gingivalis induced by AM404, respectively. Of all tested pathogens, AM404 only inhibited growth and biofilm formation of P. gingivalis. Moreover, it showed potent activity against P. gingivalis biofilms formed on titanium surfaces. A structure–activity analysis demonstrated that the unsaturated carbon chain is essential for its antibacterial activity. Importantly, AM404 was not toxic towards the tested mammalian cells up to concentrations approaching 4× the MIC. Membrane damage assays using fluorescent probes N‐phenyl‐1‐napthylamine and SYTOX green revealed that membrane permeabilization presumably is the primary antibacterial mode of action of AM404. Collectively, our results suggest that AM404 has the potential to be used for the development of new drugs specifically targeting P. gingivalis‐related infections.

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“…In a study where 236 lactic acid bacterial isolates from food were included, none of the tested strains was able to affect the growth of gram-negative periodontal pathogens, including P. gingivalis [ 42 ]. Antimicrobial agents from L. paracasei HL32 have been shown to suppress P. gingivalis growth [ 43 , 44 ], and a short biosynthetic peptide, Pep-7, was produced and demonstrated to be selectively active against P. gingivalis [ 45 ]. We show that the antimicrobial effect of the two-peptide bacteriocin PLNC8 αβ occur rapidly and is very efficient, using liposomes and viable P. gingivalis .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effects of Lactobacillus and bacteriocin PLNC8 αβ on the periodontal pathogen Porphyromonas gingivalis

et al. 2016

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BackgroundThe complications in healthcare systems associated with antibiotic-resistant microorganisms have resulted in an intense search for new effective antimicrobials. Attractive substances from which novel antibiotics may be developed are the bacteriocins. These naturally occurring peptides are generally considered to be safe and efficient at eliminating pathogenic bacteria. Among specific keystone pathogens in periodontitis, Porphyromonas gingivalis is considered to be the most important pathogen in the development and progression of chronic inflammatory disease. The aim of the present study was to investigate the antimicrobial effects of different Lactobacillus species and the two-peptide bacteriocin PLNC8 αβ on P. gingivalis.ResultsGrowth inhibition of P. gingivalis was obtained by viable Lactobacillus and culture media from L. plantarum NC8 and 44048, but not L. brevis 30670. The two-peptide bacteriocin from L. plantarum NC8 (PLNC8 αβ) was found to be efficient against P. gingivalis through binding followed by permeabilization of the membranes, using Surface plasmon resonance analysis and DNA staining with Sytox Green. Liposomal systems were acquired to verify membrane permeabilization by PLNC8 αβ. The antimicrobial activity of PLNC8 αβ was found to be rapid (1 min) and visualized by TEM to cause cellular distortion through detachment of the outer membrane and bacterial lysis.ConclusionSoluble or immobilized PLNC8 αβ bacteriocins may be used to prevent P. gingivalis colonization and subsequent pathogenicity, and thus supplement the host immune system against invading pathogens associated with periodontitis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0810-8) contains supplementary material, which is available to authorized users.

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Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

Cited by 13 publications

References 31 publications

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Repurposing AM404 for the treatment of oral infections by Porphyromonas gingivalis

Antibacterial effects of Lactobacillus and bacteriocin PLNC8 αβ on the periodontal pathogen Porphyromonas gingivalis

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