Novel Antimicrobial Peptides from the Arctic Polychaeta Nicomache minor Provide New Molecular Insight into Biological Role of the BRICHOS Domain

Panteleev, Pavel V.; Tsarev, Andrey V.; Bolosov, Ilia A.; Paramonov, Alexander S.; Marggraf, Mariana B; Sychev, Sergei V.; Шенкарев, Захар О.; Ovchinnikova, Tatiana V.

doi:10.3390/md16110401

Cited by 36 publications

(41 citation statements)

References 58 publications

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“…Recently, nicomicin-1 and -2 were identified in the artic polychaeta Nicomache minor [110]. This worm lives in the cold water, inhabiting hard tubes attached to stones [111].…”

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

“…Nicomicins consist of 33 residues (Table 1), containing BRICHOS domain in the sequences of their prepropeptide. They are characterized by many hydrophobic amino acids (51%) and a disulfide bond (Cys24 – Cys29) [110]. While Nicomicin-2 has no effect on bacteria, Nicomicin-1 exerts strong antimicrobial activity towards Gram-positive bacteria by damaging their membranes; the presence of salt impedes its activity [110].…”

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

“…They are characterized by many hydrophobic amino acids (51%) and a disulfide bond (Cys24 – Cys29) [110]. While Nicomicin-2 has no effect on bacteria, Nicomicin-1 exerts strong antimicrobial activity towards Gram-positive bacteria by damaging their membranes; the presence of salt impedes its activity [110]. Conversely, the AMP 3D structure is different from alvinellacin and arenicin and is organized into two independent regions with an α-helix at the N-terminal moiety and a six-residue loop stabilized by the disulfide bridge at the C-terminus [110].…”

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

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Worms’ Antimicrobial Peptides

et al. 2019

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Antimicrobial peptides (AMPs) are natural antibiotics produced by all living organisms. In metazoans, they act as host defense factors by eliminating microbial pathogens. But they also help to select the colonizing bacterial symbionts while coping with specific environmental challenges. Although many AMPs share common structural characteristics, for example having an overall size between 10–100 amino acids, a net positive charge, a γ-core motif, or a high content of cysteines, they greatly differ in coding sequences as a consequence of multiple parallel evolution in the face of pathogens. The majority of AMPs is specific of certain taxa or even typifying species. This is especially the case of annelids (ringed worms). Even in regions with extreme environmental conditions (polar, hydrothermal, abyssal, polluted, etc.), worms have colonized all habitats on Earth and dominated in biomass most of them while co-occurring with a large number and variety of bacteria. This review surveys the different structures and functions of AMPs that have been so far encountered in annelids and nematodes. It highlights the wide diversity of AMP primary structures and their originality that presumably mimics the highly diverse life styles and ecology of worms. From the unique system that represents marine annelids, we have studied the effect of abiotic pressures on the selection of AMPs and demonstrated the promising sources of antibiotics that they could constitute.

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“…Recently, nicomicin-1 and -2 were identified in the artic polychaeta Nicomache minor [110]. This worm lives in the cold water, inhabiting hard tubes attached to stones [111].…”

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

Section: Amps Diversity In Annelids and Nematodesmentioning

confidence: 99%

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Worms’ Antimicrobial Peptides

et al. 2019

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“…Polychaete is a marine worm that belongs to phylum Annelida. It is a small invertebrate that inhabits all places in a marine environment and contributes to a high percentage of the total macrobenthic community diversity, abundance, and biomass [ 8 , 9 ]. In Malaysia, 64 species of polychaete that come from 31 families have been recorded from 1866 to 2013 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats

Soh

Rapi

Azam

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. 1H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter.

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“…Papers included in this Special Issue deal with marine polychaeta, providing good examples of their biological potential. The peptides arenicins and nicomicins, isolated from Arenicola marina [21,22] and Nicomache minor [23], respectively, exhibited in vitro antimicrobial activity and possessed cytotoxicity against cancer cells. Arenicin was shown to modulate the human complement system [22].…”

mentioning

confidence: 99%