Novel Apoptosis Suppressor Apsup from the Baculovirus Lymantria dispar Multiple Nucleopolyhedrovirus Precludes Apoptosis by Preventing Proteolytic Processing of Initiator Caspase Dronc

Yamada, Hayato; Kitaguchi, Koji; Hamajima, Rina; Kobayashi, Michihiro; Ikeda, Motoko

doi:10.1128/jvi.02065-13

Cited by 26 publications

(18 citation statements)

References 62 publications

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“…Ld‐Apsup suppresses apoptosis by inhibiting the processing and activation of the lepidopteran initiator caspase Dronc (Yamada et al . ) (Fig. ).…”

Section: Baculovirus Host Range Factors Involved In Apoptosismentioning

confidence: 93%

“…Apsup suppresses the apoptosis of lepidopteran cells, including BM‐N, Ld652Y and Sf9 cells, triggered by p35 ‐defective recombinant AcMNPV or exposure to actinomycin D or ultraviolet light, but does not inhibit the apoptosis of Drosophila S2 cells triggered by p35 ‐defective AcMNPV (Yamada et al . ). LdMNPV Apsup (Ld‐Apsup) homologues are found mainly in NPVs but several GVs and ascoviruses also encode homologues that resemble Ld‐Apsup.…”

Section: Baculovirus Host Range Factors Involved In Apoptosismentioning

confidence: 97%

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Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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Baculoviruses, members of the family Baculoviridae, are large, enveloped viruses that contain a doublestranded circular DNA genome of 80-180 kbp, encoding 90-180 putative proteins. These viruses are exclusively pathogenic for arthropods, particularly insects, and have been developed, or are being developed, as environmentally sound pesticides and eukaryotic vectors for foreign protein expression, surface display, gene delivery for gene therapy, vaccine production and drug screening. The baculoviruses contain a set of approximately 30 core genes that are conserved among all baculovirus genomes sequenced to date. Individual baculoviruses also contain a number of lineage-or species-specific genes that have greatly impacted the diversification and evolution of baculoviruses. In this review, we first describe the general properties and biology of baculoviruses and then focus on the baculovirus genes and mechanisms involved in the replication, spread and survival of baculoviruses within the context of their diversity, evolution and insect manipulation.

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“…Ld‐Apsup suppresses apoptosis by inhibiting the processing and activation of the lepidopteran initiator caspase Dronc (Yamada et al . ) (Fig. ).…”

Section: Baculovirus Host Range Factors Involved In Apoptosismentioning

confidence: 93%

Section: Baculovirus Host Range Factors Involved In Apoptosismentioning

confidence: 97%

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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“…However, the Epiphyas postvittana MNPV (EppoM-NPV) IAP-3 is unable to inhibit apoptosis [38], and IAP-3 and IAP-2 from Lymantria dispar MNPV (LdMNPV) induced apoptosis when overexpressed [39]. It should be noted that LdMNPV carries another type of viral apoptosis suppressor, called apsup [40], which is not related to either p35 or iap.…”

Section: Which Viral Iaps Can Inhibit Apoptosis?mentioning

confidence: 97%

Viral IAPs, then and now

Clem

2015

Seminars in Cell & Developmental Biology

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“…In this study, our results demonstrated that N. bombycis inhibits apoptosis by preventing the activation of the lepidopteran mitochondrial signaling pathway. Like other types of lepidopteran insect cells [43,44], after incubation with ActD, uninfected BmN cells detach from the substrate, become blebby (data not shown) and undergo DNA fragmentation. According to the results of flow cytometry, the early apoptotic ratio of infected cells was decreased compared with that of uninfected BmN cells.…”

Section: Discussionmentioning

confidence: 76%

<italic>Nosema bombycis</italic> (Microsporidia) suppresses apoptosis in <italic>Bm</italic>N cells (<italic>Bombyx mori</italic>)

et al. 2015

ABBS

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Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.

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Novel Apoptosis Suppressor Apsup from the Baculovirus Lymantria dispar Multiple Nucleopolyhedrovirus Precludes Apoptosis by Preventing Proteolytic Processing of Initiator Caspase Dronc

Cited by 26 publications

References 62 publications

Baculoviruses: diversity, evolution and manipulation of insects

Baculoviruses: diversity, evolution and manipulation of insects

Viral IAPs, then and now

<italic>Nosema bombycis</italic> (Microsporidia) suppresses apoptosis in <italic>Bm</italic>N cells (<italic>Bombyx mori</italic>)

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Novel Apoptosis Suppressor Apsup from the Baculovirus Lymantria dispar Multiple Nucleopolyhedrovirus Precludes Apoptosis by Preventing Proteolytic Processing of Initiator Caspase Dronc

Cited by 26 publications

References 62 publications

Baculoviruses: diversity, evolution and manipulation of insects

Baculoviruses: diversity, evolution and manipulation of insects

Viral IAPs, then and now

&lt;italic&gt;Nosema bombycis&lt;/italic&gt; (Microsporidia) suppresses apoptosis in &lt;italic&gt;Bm&lt;/italic&gt;N cells (&lt;italic&gt;Bombyx mori&lt;/italic&gt;)

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<italic>Nosema bombycis</italic> (Microsporidia) suppresses apoptosis in <italic>Bm</italic>N cells (<italic>Bombyx mori</italic>)