Novel application of imaging surface plasmon resonance for <i>in situ</i> studies of the surface exploration of marine organisms

Andersson, Olof; Ekblad, Tobias; Aldred, Nick; Clare, Anthony S.; Liedberg, Bo

doi:10.1116/1.3274060

Cited by 28 publications

(34 citation statements)

References 17 publications

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“…Touchdowns of the antennules can be visualized and it is possible to observe whether or not adhesive remains on the surface. Cyprids of the species Semibalanus balanoides left footprints on bare gold surfaces while on mOEG only touchdown events were detected [107]. Especially noteworthy from a surface science point of view is the mechanism of attachment and detachment of the antennules, which has similarities with the detachment of protein loaded AFM tips.…”

Section: Footprints Of Walking Cyprids Visualized By Imaging Surface mentioning

confidence: 97%

“…Recently, imaging Surface Plasmon Resonance (iSPR) was applied by the Liedberg group to observe and quantify the adhesive deposition during ''walking'' of the cyprids (Fig. 12) [107]. Touchdowns of the antennules can be visualized and it is possible to observe whether or not adhesive remains on the surface.…”

Section: Footprints Of Walking Cyprids Visualized By Imaging Surface mentioning

confidence: 99%

“…The magnified view of the contact point illustrates schematically the probing depth of SPR of a few hundreds of nanometers (reproduced from Ref. [107] (Fig. 1, Ó Springer 2009) with kind permission from Springer Science and Business Media) Fig.…”

Section: Footprints Of Walking Cyprids Visualized By Imaging Surface mentioning

confidence: 99%

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Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Rosenhahn

Sendra

2012

Biointerphases

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This review article summarizes some recent insights into the strategies used by marine organisms to select surfaces for colonization. While larger organisms rely on their sensory machinery to select surfaces, smaller microorganisms developed less complex but still effective ways to probe interfaces. Two examples, zoospores of algae and barnacle larvae, are discussed and both appear to have build-in test mechanisms to distinguish surfaces with different physicochemical properties. Some systematic studies on the influence of surface cues on exploration, settlement and adhesion are summarized. The intriguing notion that surface colonization resembles a parallelized surface sensing event is discussed towards its complementarity with conventional surface analytical tools. The strategy to populate only selected surfaces seems advantageous as waves, currents and storms constantly challenge adherent soft and hard fouling organism.

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Section: Footprints Of Walking Cyprids Visualized By Imaging Surface mentioning

confidence: 97%

Section: Footprints Of Walking Cyprids Visualized By Imaging Surface mentioning

confidence: 99%

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Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Rosenhahn

Sendra

2012

Biointerphases

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“…This interaction includes a temporary adhesive secreted at the terminus of the antennules, which enables temporary adhesion , Aldred, Li et al 2010, Aldred, Høeg et al 2013. As reported by Andersson et al (Andersson et al 2009), the application of imaging surface plasmon resonance microscopy (iSPR microscopy) allows quantitative analysis of the interaction of cyprids and their temporary adhesive with the surfaces. iSPR microscopy is an imaging optical transducer technique which measures refractive index changes of thin dielectric layers (de Bruijn et al 1993), which can be translated into adsorbed amounts, or layer thicknesses.…”

Section: Introductionmentioning

confidence: 95%

“…In this way dynamics of the swimming motion, detectable from 3D motility data, and of cyprid-surface interaction and exploration behavior can directly be correlated with iSPR. For these experiments, we used barnacle cypris larvae of the species Balanus amphitrite, which are considerably smaller than the Semibalanus balanoides larvae used in previous iSPR experiments (Andersson et al 2009, and whose footprints are more challenging to detect and record. Using two different self-assembled monolayers (SAMs) to vary the surface chemistry, we demonstrate that both surface interactions and bulk swimming patterns vary with the surface chemistry.…”

Section: Introductionmentioning

confidence: 99%

Imaging SPR combined with stereoscopic 3D tracking to study barnacle cyprid–surface interactions

et al. 2016

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Barnacle larvae (cyprids) explore surfaces to identify suitable settlement sites. This process is selective, and cyprids respond to numerous surface cues. To better understand the settlement process, it is desirable to simultaneously monitor both the surface exploration behavior and any close interactions with the surface. Stereoscopic 3D tracking of the cyprids provides quantitative access to surface exploration and pre-settlement rituals. Imaging surface plasmon resonance (SPR) reveals any interactions with the surfaces, such as surface inspection during bipedal walking and deposition of temporary adhesives. We report on a combination of both techniques to bring together information on swimming behavior in the vicinity of the interface and physical interactions of the cyprid with the surface. The technical requirements for the setup are described, and we applied the setup to cyprids of Balanus amphitrite. Initial data shows the applicability of the combined setup to correlate exploration and touchdown events on surfaces with different chemical termination.

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Patterned Hydrogels for Controlled Platelet Adhesion from Whole Blood and Plasma

Ekblad¹,

Faxälv²,

Andersson³

et al. 2010

Adv Funct Materials

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This work describes the preparation and properties of hydrogel surface chemistries enabling controlled and well‐defined cell adhesion. The hydrogels may be prepared directly on plastic substrates, such as polystyrene slides or dishes, using a quick and experimentally simple photopolymerization process, compatible with photolithographic and microfluidic patterning methods. The intended application for these materials is as substrates for diagnostic cell adhesion assays, particularly for the analysis of human platelet function. The non‐specific adsorption of fibrinogen, a platelet adhesion promoting protein, is shown to be completely inhibited by the hydrogel, provided that the film thickness is sufficient (>5 nm). This allows the hydrogel to be used as a matrix for presenting selected bioactive ligands without risking interference from non‐specifically adsorbed platelet adhesion factors, even in undiluted whole blood and blood plasma. This concept is demonstrated by preparing patterns of proteins on hydrogel surfaces, resulting in highly controlled platelet adhesion. Further insights into the protein immobilization and platelet adhesion processes are provided by studies using imaging surface plasmon resonance. The hydrogel surfaces used in this work appear to provide an ideal platform for cell adhesion studies of platelets, and potentially also for other cell types.

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Novel application of imaging surface plasmon resonance for in situ studies of the surface exploration of marine organisms

Cited by 28 publications

References 17 publications

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Imaging SPR combined with stereoscopic 3D tracking to study barnacle cyprid–surface interactions

Patterned Hydrogels for Controlled Platelet Adhesion from Whole Blood and Plasma

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