A typical glucose sensor is a glucose oxidase (GOx) enzyme-based sensor due to its high sensitivity and selectivity. However, it has difficulty activating and stabilizing the enzyme. To enhance the stability of GOx-based sensors, chitosan is added to ZnS-doped Mn nanoparticles. Chitosan stabilizes the ZnS-doped Mn particles and can induce charge processes such as creation, transfer, and separation at the polymer interface and nanoparticles' interface. Hence, it is a strong candidate for electrochemical glucose sensing applications. This letter reports the impedance biosensor based on ZnS-doped Mn-capped chitosan nanomaterials and microinterdigitated electrodes. The glucose sensors' high sensitivity, accuracy, and stability are obtained in the range of 2 to 10 mM. In addition, an application of the Canny edge detection to enhance the limit of detection is demonstrated using experimental data.