Novel approach based on GQD-PHB as anchoring platform for the development of SARS-CoV-2 electrochemical immunosensor

Martins, Gustavo Celestino; Gogola, Jeferson L.; Budni, Lucas H.; Papi, Maurício A.; Bom, Maritza A.T.; Budel, Maria L.T.; Souza, Emanuel Maltempi de; Müller-Santos, Marcelo; Beirão, Breno Castello Branco; Banks, Craig E.; Marcolino‐Júnior, Luiz H.; Bergamini, Márcio F.

doi:10.1016/j.aca.2022.340442

Cited by 16 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A new method was devised by Martins et al [75] for constructing immunosensors by attaching biological material to a screen-printed carbon electrode (SPE) that has been modified with electrodeposited Graphene Quantum Dots (GQD) and polyhydroxybutyric acid (PHB). The resultant electrode was employed as a functional substrate to recognize SARS-CoV-2 spike protein RBD to detect Anti-S antibodies with a LOD value of 100 ng/mL (Figure 8).…”

Section: Using Graphene Quantum Dotsmentioning

confidence: 99%

Graphene-Based Electrochemical Nano-Biosensors for Detection of SARS-CoV-2

Sengupta¹,

Hussain

2023

Inorganics

View full text Add to dashboard Cite

COVID-19, a viral respiratory illness, is caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), which was first identified in Wuhan, China, in 2019 and rapidly spread worldwide. Testing and isolation were essential to control the virus’s transmission due to the severity of the disease. In this context, there is a global interest in the feasibility of employing nano-biosensors, especially those using graphene as a key material, for the real-time detection of the virus. The exceptional properties of graphene and the outstanding performance of nano-biosensors in identifying various viruses prompted a feasibility check on this technology. This paper focuses on the recent advances in using graphene-based electrochemical biosensors for sensing the SARS-CoV-2 virus. Specifically, it reviews various types of electrochemical biosensors, including amperometric, potentiometric, and impedimetric biosensors, and discusses the current challenges associated with biosensors for SARS-CoV-2 detection. The conclusion of this review discusses future directions in the field of electrochemical biosensors for SARS-CoV-2 detection, underscoring the importance of continued research and development in this domain.

show abstract

Section: Using Graphene Quantum Dotsmentioning

confidence: 99%

Graphene-Based Electrochemical Nano-Biosensors for Detection of SARS-CoV-2

Sengupta¹,

Hussain

2023

Inorganics

View full text Add to dashboard Cite

show abstract

“…These results, in combination with successful clinical trials, show that the bionanocomposite scaffold could be implemented for antidiabetic devices, which should "potentially improve the quality of life for millions of patients" [38]. Much more sophisticated G-PHB nanocomposites with embedded graphene quantum dots have recently demonstrated an ultimately successful recognition of receptor binding domains from the coronavirus spike protein to indicate the specific antibody as the witness of the virus infection [12].…”

Section: Introductionmentioning

confidence: 98%

“…This polyester is close to polypropylene in a number of physicochemical characteristics [11]. It has satisfactory thermal, mechanical, and barrier properties, but these characteristics require significant improvement in the situation when innovative platforms, implants, and devices for the implementation in the diverse areas of human activity based on PHB are designed [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Biocomposites Based on Electrospun Fibers of Poly(3-hydroxybutyrate) and Nanoplatelets of Graphene Oxide: Thermal Characteristics and Segmental Dynamics at Hydrothermal and Ozonation Impact

Karpova,

Olkhov,

Varyan

et al. 2023

Polymers

View full text Add to dashboard Cite

In order to create new biodegradable nanocomposites for biomedicine, packaging, and environmentally effective adsorbents, ultra-thin composite fibers consisting of poly(3-hydroxybutyrate) (PHB) and graphene oxide (GO) were obtained by electrospinning. Comprehensive studies of ultrathin fibers combining thermal characteristics, dynamic electron paramagnetic resonance (ESR) probe measurements, and scanning electron microscopy (SEM) were carried out. It is shown that at the addition of 0.05, 0.1, 0.3, and 1% OG, the morphology and geometry of the fibers and their thermal and dynamic characteristics depend on the composite content. The features of the crystalline and amorphous structure of the PHB fibers were investigated by the ESR and DSC methods. For all compositions of PHB/GO, a nonlinear dependence of the correlation time of molecular mobility TEMPO probe (τ) and enthalpy of biopolyether melting (ΔH) is observed. The influence of external factors on the structural-dynamic properties of the composite fiber, such as hydrothermal exposure of samples in aqueous medium at 70 °C and ozonolysis, leads to extreme dependencies of τ and ΔH, which reflect two processes affecting the structure in opposite ways. The plasticizing effect of water leads to thermal destruction of the orientation of the pass-through chains in the amorphous regions of PHB and a subsequent decrease in the crystalline phase, and the aggregation of GO nanoplates into associates, reducing the number of GO-macromolecule contacts, thus increasing segmental mobility, as confirmed by decreasing τ values. The obtained PHB/GO fibrillar composites should find application in the future for the creation of new therapeutic and packaging systems with improved biocompatibility and high-barrier properties.

show abstract

“…These immunoassays have the limitations that the antibodies are expensive, and there can be a significant deviation in detection. Electrochemical analyses are increasingly being used as rapid tests since this methodology provides a straightforward and cheap arrangement due to the possibility of a label-free detection [ 6 ]. For this purpose, electrochemical genosensors combine the inherent specificity of Watson–Crick base-pairing with the high sensitivity of electrochemical sensors and thus sustain the development of the next generation of POC tests.…”

Section: Introductionmentioning

confidence: 99%