Novel approaches to determination of PAHs and halogenated POPs in canned fish

Abstract

Drábová L., Pulkrabová J., Kalachová K., Hradecký J., Suchanová M., Tomaniová M., Kocourek V., Hajšlová J. (2011): Novel approaches to determination of PAhs and halogenated PoPs in canned fish. Czech J. Food Sci., 29: 498-507. A simple method is described for simultaneous isolation of 7 indicator polychlorinated biphenyls (PCBs), 10 polybrominated diphenyl ethers (PBDEs), 22 organochlorine pesticides (OCPs), and 16 polycyclic aromatic hydrocarbons (16 EU PAHs). The sample preparation procedure, including a pressurised liquid extraction (PLE) followed by gel permeation chromatography (GPC) for the selective isolation of the target compounds, was optimised and validated. For the final identification/quantitation of the target PCBs, PBDEs, OCPs, and PAHs, gas chromatography (GC) coupled to a high speed time-of-flight mass spectrometer (TOF MS) was used. The performance characteristics of the procedure were assessed including the recoveries (86-118% for PCBs, 73-113% for PBDEs, 71-113% for OCPs, and 85-111% for PAHs), repeatabilities (3-12% PCBs, 3-9% PBDEs, 1-11% OCPs and 3-10% PAHs), and limits of quantitation (LOQs -0.5 µg/kg PCBs, 0.1-0.3 µg/kg PBDEs, 0.1-0.5 µg/kg OCPs, and 0.03-0.1 µg/kg PAHs). Within the follow-up study, this method will be used for the monitoring of contamination of canned fish and sea food products available at the Czech market.

Application of gas and liquid chromatography coupled to time‐of‐flight mass spectrometry in pesticides: Multiresidue analysis

“…A multiresidue pesticide method based on QuEChERS and GC–MS and multidimensional GC‐TOF for determination of 166 pesticides in spinach was reported (Wong, Zhang, Hayward, & Kai‐Meng, ). An optimization and validation of extraction procedure based on pressurized liquid extraction and gel permeation chromatography followed by GC‐TOF‐MS for analysis of Polychlorinated biphenyls (PCBs), Polybrominated diphenyl ethers (PBDEs), Organochlorine pesticides (OCPs) and Polycyclic aromatic hydrocarbon (PAHs) in canned fish and seafood products was demonstrated (Drábová et al, ).…”

Determination of Pesticide Residues in Food of Animal Origin

“…However, high solvent consumption and laborious sample-handling can be expected by using these methodologies. Therefore, an automated extraction technique, as PLE, has been applied for the extraction of milk, egg and meat 13,14 , liver and fish 15,16 , and honey 17 . The use of elevated temperatures and pressure increases diffusion and desorption rates.…”

“…Gel permeation chromatography (GPC) is a non-destructive treatment applied for lipid elimination. GPC is mainly performed either in automated systems 14,16 or by gravity flow columns 9 . The current use of pre-packed polystyrene-divinyl benzene based high performance GPC columns has resulted in higher separation efficiencies, improved reproducibility and lower solvent consumption as compared to manually packed columns.…”

“…The time-of-flight detector (TOF) has been applied in different matrices like fish 14 and honey, honeybee and pollen 76 for the determination of multiple classes of pesticides. Both of them operated in the EI mode and employed a BPX-50 column 14 and a DB-XLB column 76 .…”

“…The time-of-flight detector (TOF) has been applied in different matrices like fish 14 and honey, honeybee and pollen 76 for the determination of multiple classes of pesticides. Both of them operated in the EI mode and employed a BPX-50 column 14 and a DB-XLB column 76 . On the other hand, to improve the drawbacks of the TOF detector, a hybrid system, consisted on a quadrupole/TOF (Q-TOF), has been applied for the determination of 50 pesticides in meat samples 34 .…”