The stratum corneum of the outermost skin is an important barrier impeding transdermal
permeation, and permeation enhancers can reduce the barrier resistance
of the stratum corneum and enhance the permeation of drugs in tissues.
The optical imaging depth, signal intensity, and scattering
coefficient variation rules of skin tissues in time dimension are
obtained by using optical coherence tomography (OCT). The effect of
optical clearing agents (OCAs) on OCT imaging is obtained by
quantitatively analyzing the changes in the optical properties of
tissues. D-fructose, one of the monosaccharides, and sucrose, one of
the disaccharides, were selected for the ex vivo
optical clearing experiments on pig skin tissues utilizing the
dimethyl sulfoxide (DMSO) carrier effect. We find that DMSO synergized
with sugars applied to skin tissue has a more significant increase in
the optical imaging depth and signal intensity, and a reduction in the
scattering coefficient with an increasing concentration of DMSO. DMSO
with a high concentration and D-fructose with saturated concentration
(10:1; v/v) effectively reduce light attenuation in OCT imaging and
improve the image quality. This operation will also shorten the
application time to minimize skin damage from hyperosmotic agents.