Novel Bartonella Species in Insectivorous Bats, Northern China

Han, Hui; Wen, Hong Ling; Zhao, Li; Liu, Jian Wei; Luo, Li; Zhou, Chunkui; Qin, Xiang Rong; Zhu, Ye; Zheng, Xue Xing; Yu, Xue Jie

doi:10.1371/journal.pone.0167915

Cited by 28 publications

(32 citation statements)

References 36 publications

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“…Reports of Bartonella spp. infections are known from blood of bats (Judson et al 2015, Han et al 2017, Urushadze et al 2017), from tissues (Bai et al 2017, Stuckey et al 2017) and from their associated ectoparasites (Morse et al 2012, Davoust et al 2016, Sándor et al 2018, Hornok et al 2019). In our study this pathogen group was found in low proportions, in two samples (2 out of 10, 20%), but the PCR targeting the ITS gene of Bartonella spp.…”

Section: Discussionmentioning

confidence: 99%

The heart microbiome of insectivorous bats from Central and South Eastern Europe

Corduneanu

Mihalca

Sándor

et al. 2020

Preprint

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Host associated microbiome not only may affect the individual health-status or provide insights into the species- or group specific bacterial communities but may act as early warning signs in the assessment of zoonotic reservoirs, offering clues to predict, prevent and control possible episodes of emerging zoonoses. Bats may be carriers and reservoirs of multiple pathogens such as viruses, bacteria and parasites, showing in the same time robust immunity against many of them. The microbiota plays a fundamental role on the induction, training and function of the host immune system and the immune system has largely evolved in order to maintain the symbiotic relationship of the host with these diverse microbes. Thus, expanding our knowledge on bat-associated microbiome it can be usefully in understanding bats’ outstanding immune capacities. The aim of this study was to investigate the presence of different bacterial communities in heart tissue of insectivorous bats, Nyctalus noctula, Pipistrellus pipistrellus and Rhinoplophus hipposideros, from Central and Eastern Europe using high-throughput sequencing of variable regions of the 16S rRNA. In addition, species-specific PCRs were used to validate the presence of the vector-borne pathogens Bartonella spp. and Rickettsia spp. In this study we identified a wide variety of bacterial groups, with the most abundant phyla being Proteobacteria and Firmicutes. The results showed that at individual level, the year or location had no effect on the diversity and composition of the microbiome, however host species determined both structure and abundance of the bacterial community. We report the presence of vector-borne bacteria Bartonella spp. in samples of N. noctula and indications of Rickettsia spp. in R. hipposideros. Our results provide a first insight into the bacterial community found in heart tissue of bats from Central and South Eastern Europe.

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Section: Discussionmentioning

confidence: 99%

The heart microbiome of insectivorous bats from Central and South Eastern Europe

Corduneanu

Mihalca

Sándor

et al. 2020

Preprint

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“…Currently, 35 species of Bartonella have been identified (http://www.bacterio.net/index .html) and have international standing in nomenclature, and other species have been proposed, several of which are classified as "Candidatus" species (80). New species of Bartonella continue to be discovered, but frequently only partial genetic data obtained by molecular analysis are available (81)(82)(83). B. rochalimae was reported in 2007 to have caused an Oroya fever-like disease in a traveler returning from Peru (57), and a retrospective study also identified this species in the blood of a Peruvian patient with Carrion's disease (84).…”

Section: Bartonellaceaementioning

confidence: 99%

Carrion's Disease: the Sound of Silence

2018

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Carrion's disease (CD) is a neglected biphasic vector-borne illness related to . It is found in the Andean valleys and is transmitted mainly by members of the genus but also by blood transfusions and from mother to child. The acute phase, Oroya fever, presents severe anemia and fever. The lethality is high in the absence of adequate treatment, despite the organism being susceptible to most antibiotics. Partial immunity is developed after infection by , resulting in high numbers of asymptomatic carriers. Following infection there is the chronic phase, Peruvian warts, involving abnormal proliferation of the endothelial cells. Despite potentially being eradicable, CD has been expanded due to human migration and geographical expansion of the vector. Moreover, studies have demonstrated the risk of the development of antimicrobial resistance. These findings, together with the description of new species producing CD-like infections, the presence of undescribed potential vectors in new areas, the lack of adequate diagnostic tools and knowledge of the immunology and bacterial pathogenesis of CD, and poor international visibility, have led to the risk of increasing the potential expansion of resistant strains which will challenge current treatment schemes as well as the possible appearance of CD in areas where it is not endemic.

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“…Details regarding anesthetization of bats and tissue sampling were described previously ( 5 ). We collected the kidneys, storing them at −80°C until analysis.…”

Section: The Studymentioning

confidence: 99%