Novel bi-layered dressing patches constructed with radially-oriented nanofibrous pattern and herbal compound-loaded hydrogel for accelerated diabetic wound healing

Wu, Shaohua; Zhao, Wenwen; Sun, Mingchao; He, Peng; Lv, Hongyu; Wang, Qiuyu; Zhang, Shuo; Wu, Qian; Ling, Peixue; Chen, Shaojuan; Ma, Jing

doi:10.1016/j.apmt.2022.101542

Cited by 57 publications

(39 citation statements)

References 64 publications

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“…Poly(lactic acid) is biodegradable and easy to treat. It is a kind of green and environment-friendly material [ 14 , 15 ]. It has been widely used in filtration, adsorption of heavy metal ions, and biomedicine [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials

Yang

Lu³

et al. 2022

Nanomaterials

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Crude oil spills seriously harm the ocean environment and endanger the health of various animals and plants. In the present study, a totally biodegradable polymer, poly(L-lactic acid) (PLLA), was employed to fabricate highly porous oil absorbent nanofibrous materials by using a combination of electrospinning technique and subsequent acetone treatment. We systematically investigated how the electrospinning parameters affected formation of the porous structure of PLLA nanofibers and demonstrated that PLLA nanofibers with decreased and uniform diameter and improved porosity could be rapidly prepared by adjusting solution parameters and spinning parameters. We also demonstrated that the acetone treatment could obviously enhance the pore diameter and specific surface area of as-optimized electrospun PLLA nanofibers. The acetone treatment could also improve the hydrophobic property of as-treated PLLA nanofiber membranes. All these led to a significant increase in oil absorption performance. Through our research, it was found that the oil absorption of PLLA nanofiber membrane increased by more than double after being treated with acetone and the oil retention rate was also improved slightly.

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Section: Introductionmentioning

confidence: 99%

Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials

Yang

Lu³

et al. 2022

Nanomaterials

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“…An ideal wound dressing should have effective inflammatory regulation function, especially for the treatment of chronic wounds caused by diabetic ulcers, given that long-term chronic inflammation is one of the key reasons to hold back the healing process of diabetic wounds [ 29 , 41 ]. LPS-activated RAW 264.7 cells were utilized to determine the anti-inflammatory property of the CS-PVA-0.2UA nanofiber mat.…”

Section: Resultsmentioning

confidence: 99%

“…The macrophage is a prominent inflammatory cell and plays a crucial role in the early inflammatory stage of wound healing [ 29 , 47 ]. Compared with the normal wounds, the macrophages in the diabetic wounds commonly exhibited a high expression level of the pro-inflammatory M1 phenotype, rather than the pro-healing M2 phenotype, which suppressed the transition from the inflammatory phase to the proliferative phase, finally leading to the generation of chronic hard-to-heal wounds [ 24 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…The in vivo animal study was approved by the Qingdao University Animal Research Committee. The mouse model with type I diabetic disease was established according to our previous studies [ 19 , 29 ]. The surgical procedure was performed under general anesthesia.…”

Section: Materials and Experimental Methodsmentioning

confidence: 99%

“…Philip et al demonstrated that the electrospinning dressing loaded with growth factor TGF-β helped to recruit inflammatory cells to the wound site, deactivated the superoxide ions, and promoted granulation tissue formation [ 28 ]. Wu et al found that the use of antioxidant ingredient manganese dioxide in the fabrication of dressing materials could effectively reduce the oxidative stress by eliminating ROS and replenishing oxygen to promote diabetic wound healing [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Electrospun Chitosan–Polyvinyl Alcohol Nanofiber Dressings Loaded with Bioactive Ursolic Acid Promoting Diabetic Wound Healing

Zhao

et al. 2022

Nanomaterials

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The design and development of novel dressing materials are urgently required for the treatment of chronic wounds caused by diabetic ulcers in clinics. In this study, ursolic acid (UA) extracted from Chinese herbal plants was encapsulated into electrospun nanofibers made from a blend of chitosan (CS) and polyvinyl alcohol (PVA) to generate innovative CS-PVA-UA dressings for diabetic wound treatment. The as-prepared CS-PVA-UA nanofiber mats exhibited randomly aligned fiber morphology with the mean fiber diameters in the range of 100–200 nm, possessing great morphological resemblance to the collagen fibrils which exist in the native skin extracellular matrix (ECM). In addition, the CS-PVA-UA nanofiber mats were found to possess good surface hydrophilicity and wettability, and sustained UA release behavior. The in vitro biological tests showed that the high concentration of UA could lead to slight cytotoxicity. It was also found that the CS-PVA-UA nanofiber dressings could significantly reduce the M1 phenotypic transition of macrophages that was even stimulated by lipopolysaccharide (LPS) and could effectively restore the M2 polarization of macrophages to shorten the inflammatory period. Moreover, the appropriate introduction of UA into CS-PVA nanofibers decreased the release levels of TNF-α and IL-6 inflammatory factors, and suppressed oxidative stress responses by reducing the generation of reactive oxygen species (ROS) as well. The results from mouse hepatic hemorrhage displayed that CS-PVA-UA nanofiber dressing possessed excellent hemostatic performance. The in vivo animal experiments displayed that the CS-PVA-UA nanofiber dressing could improve the closure rate, and also promote the revascularization and re-epithelization, as well as the deposition and remodeling of collagen matrix and the regeneration of hair follicles for diabetic wounds. Specifically, the mean contraction rate of diabetic wounds using CS-PVA-UA nanofiber dressing could reach 99.8% after 18 days of treatment. In summary, our present study offers a promising nanofibrous dressing candidate with multiple biological functions, including anti-inflammation, antioxidation, pro-angiogenesis, and hemostasis functions, for the treatment of hard-to-heal diabetic wounds.

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Four‐Arm Polymer‐Guided Formation of Curcumin‐Loaded Flower‐Like Porous Microspheres as Injectable Cell Carriers for Diabetic Wound Healing

Yuan

Yang

et al. 2023

Adv Healthcare Materials

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Stem cell injection is an effective approach for treating diabetic wounds; however, shear stress during injections can negatively affect their stemness and cell growth. Cell‐laden porous microspheres could provide shelter for stem cells. We herein designed curcumin‐loaded flower‐like porous microspheres (CFPM) by combining phase inversion emulsification with thermally induced phase separation‐guided four‐arm poly(L‐lactic acid) (B‐PLLA). Notably, the CFPM showed a well‐defined surface topography and inner structure, ensuring a high surface area to enable the incorporation and delivery of a large amount of stem cells and curcumin. The stem cell‐carrying CFPM (BMSC@CFPM) maintained the proliferation, retention and stemness of BMSC, which, in combination with their sustainable curcumin release, facilitated the endogenous production of growth/proangiogenic factors and offered a local anti‐inflammatory function. An in vivo bioluminescence assay demonstrated that BMSC@CFPM could significantly increase the retention and survival of BMSC in wound sites. Accordingly, BMSC@CFPM, with no significant systemic toxicity, could significantly accelerate diabetic wound healing by promoting angiogenesis, collagen reconstruction and M2 macrophage polarization. RNA sequencing further unveiled the mechanisms by which BMSC@CFPM promoted diabetic wound healing by increasing the expression of growth factors and enhancing angiogenesis through the JAK/STAT pathway. Overall, BMSC@CFPM represents a potential therapeutic tool for diabetic wound healing.This article is protected by copyright. All rights reserved

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Novel bi-layered dressing patches constructed with radially-oriented nanofibrous pattern and herbal compound-loaded hydrogel for accelerated diabetic wound healing

Cited by 57 publications

References 64 publications

Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials

Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials

Electrospun Chitosan–Polyvinyl Alcohol Nanofiber Dressings Loaded with Bioactive Ursolic Acid Promoting Diabetic Wound Healing

Four‐Arm Polymer‐Guided Formation of Curcumin‐Loaded Flower‐Like Porous Microspheres as Injectable Cell Carriers for Diabetic Wound Healing

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