Novel biaxial tensile test for studying aortic failure phenomena at a microscopic level

Abstract

BackgroundAn aortic aneurysm is a local dilation of the aorta, which tends to expand and often results in a fatal rupture. Although larger aneurysms have a greater risk of rupture, some small aneurysms also rupture. Since the mechanism of aortic rupture is not well understood, clarification of the microstructure influencing the failure to rupture is important. Since aortic tissues are stretched biaxially in vivo, we developed a technique to microscopically observe the failure of an aortic rupture during biaxial stretch.MethodsA thinly sliced porcine thoracic aortic specimen was adhered to a circular frame and pushed onto a cylinder with a smaller diameter to stretch the specimen biaxially. To induce failure to rupture at the center, the specimen was thinned at the center of the hole as follows: the specimen was frozen while being compressed with metal plates having holes, which were 3 mm in diameter at their centers; the specimen was then sliced at 50-μm intervals and thawed.ResultsThe ratio of the thickness at the center to the peripheral area was 99.5% for uncompressed specimens. The ratio decreased with an increase in the compression ratio εc and was 47.3% for specimens with εc = 40%. All specimens could be stretched until failure to rupture. The probability for crack initiation within the cylinder was <30% and 100% for specimens with εc <10% and εc >30%, respectively. Among specimens ruptured within the cylinder, 93% of those obtained from the mid-media showed crack initiation at the thin center area.ConclusionsAortic tissues were successfully stretched biaxially until failure, and their crack initiation points were successfully observed under a microscope. This could be a very useful and powerful method for clarifying the mechanism of aortic rupture. We are planning to use this technique for a detailed investigation of events occurring at the point of failure when the crack initiates in the aortic aneurysm wall.

Age-related distensibility and histology of the ascending aorta in elderly patients with acute aortic dissection

“…However, a better control of the storage time for the specimens would suppress the effects of freezing on the distensibility (Langerak et al, 2001;Goh et al, 2010;Sugita and Matsumoto, 2013).…”

Age-related vascular stiffening: causes and consequences

“…Recent experiments have used biaxial testing devices to study blood vessels ( Vande Geest et al, 2005 ; Zemánek et al, 2009 ; Haskett et al, 2010 ), which are more representative of in vivo mechanical loading. As examples, biaxial testing has been used to microscopically observe the failure point of artery rupture ( Sugital and Matsumoto, 2013 ) and to show that the arterial wall is stiffer in the circumferential direction compared to the axial direction ( Shafigh et al, 2013 ).…”

Biomechanics of aortic wall failure with a focus on dissection and aneurysm: A review

“…Following the protocol reported by Sugita & Matsumoto [135], recently Sugita & Matsumoto [136] performed biaxial extension tests on thinly sliced porcine thoracic aortas with a reduced cross section in the center and reported a heterogeneous deformation field in terms of strains similar to Sugita & Matsumoto [134]. Strain distribution and the collagen realignment were similar between the crack initiation sites and the remaining tissue sample, in contrast to the idea behind the maximum principal strain failure criterion.…”