Novel binding sites of 15-deoxy-Δ12,14-prostaglandin J2 in plasma membranes from primary rat cortical neurons

Anti-heat Shock 70 kDa Protein Antibody Induced Neuronal Cell Death

“…15d-PGJ 2 induces neuronal cell death via apoptosis. 23,24) Apoptosis is accompanied with plasma membrane blebs, caspase activation and chromatin condensation. The anti-Hsp70 antibody did not condense chromatin (Supplementary Fig.…”

“…22) Neither PPARγ nor its membrane receptor, chemoattractant receptor homologous molecule expressed on T-helper 2 (Th2) cells (CRTH2), was involved in 15d-PGJ 2 -induced neurotoxicity. 23) 15d-PGJ 2 activated caspase and induced neuronal apoptosis. 23,24) This apoptosis is accompanied with activation of caspase 24) and inhibition of ubiquitinproteasome pathway (UPP).…”

“…23) 15d-PGJ 2 activated caspase and induced neuronal apoptosis. 23,24) This apoptosis is accompanied with activation of caspase 24) and inhibition of ubiquitinproteasome pathway (UPP). 25) UPP is responsible for the degradation of mutant or misfolded proteins in cells and plays a critical role in maintaining cell homeostasis.…”

15-Deoxy-delta12,14-prostaglandin J2, a neuroprotectant or a neurotoxicant?

“…Effects of PGJ2 on several kinds of cells, including cancer cells, vascular smooth muscles, and astrocyte (Chen et al, 2005;Giri et al, 2004;Hashimoto et al, 2004;Ichiki et al, 2004;Takeda et al, 2001;Zhao et al, 2004), have been being investigated. Although there are several reports about its effects on neuronal cells (Aoun et al, 2003;Heneka et al, 1999;Li et al, 2004a,b;Rohn et al, 2001;Yagami et al, 2003;Zhuang et al, 2003), the precise effects of 15d-PGJ2 on neuronal cells have not yet been clearly established. In the reports that PGJ2 was neurotoxic (Li et al, 2004a,b;Rohn et al, 2001), as the mechanism of neurotoxic effect of 15d-PGJ2, it has been suggested that 15d-PGJ2 enhances COX-2 expression (Li et al, 2004a), inhibits the ubiquitin hydrolase UCH-L1 and elicits ubiquitinprotein aggregation without proteasome inhibition (Li et al, 2004b), and induces apoptosis (Rohn et al, 2001).…”

“…There have been several reports about effects of PGJ2 on neuronal cells (Aoun et al, 2003;Heneka et al, 1999;Li et al, 2004a,b;Rohn et al, 2001;Yagami et al, 2003;Zhuang et al, 2003). Nevertheless, its precise effects on neuronal cells have not yet been clearly established.…”