Novel biocatalyst for optimal biodiesel production from diatoms

Saranya, G.; Ramachandra, T. V.

doi:10.1016/j.renene.2020.02.053

Cited by 32 publications

(15 citation statements)

References 95 publications

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“…Triacylglycerols in C. cryptica and T. weissflogii could account for up to 88% and 82% of total glycerolipids, offering these diatoms as attractive candidates for up-scalable production of biofuels . Besides, the purified lipase enzyme with good stability obtained from Cladosporium tenuissimum fungi was utilized as a biocatalyst for the enzymatic transesterification of oil realized from Nitzschia punctata diatom; this biocatalyst exhibited high productivity of biodiesel formation …”

Section: Applications Of Diatomsmentioning

confidence: 99%

“…115 Besides, the purified lipase enzyme with good stability obtained from Cladosporium tenuissimum fungi was utilized as a biocatalyst for the enzymatic transesterification of oil realized from Nitzschia punctata diatom; this biocatalyst exhibited high productivity of biodiesel formation. 116 Phaeodactylum tricornutum diatoms were explored for the nutrient elimination as well as biodiesel production simultaneously using various growth mediums, including seawater and wastewater; significant efficiency of nutrient elimination and high lipid production (54.76 mg/L/day) could be acquired. 117 In another study, Chaffin et al 118 reported that Fragilaria capucina diatom could accumulate lipid to excessive levels and tolerance various temperatures, permitting a significant rate of growth and thus can be considered as suitable candidate for production of biofuels; however, one of the important challenges is industrial and up-scalable applications of diatoms, which needs more improvements, optimization of procedures, and analytical studies.…”

Section: Applications Of Diatomsmentioning

confidence: 99%

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Diatoms with Invaluable Applications in Nanotechnology, Biotechnology, and Biomedicine: Recent Advances

Rabiee

Khatami

Soufi

et al. 2021

ACS Biomater. Sci. Eng.

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Diatoms are unicellular microalga found in soil and almost every aquatic environment (marine and fresh water). Biogenic silica and diatoms are attractive for biotechnological and industrial applications, especially in the field of biomedicine, industrial/synthetic manufacturing processes, and biomedical/pharmaceutical sciences. Deposition of silica by diatoms allows them to create micro- or nanoscale structures which may be utilized in nanomedicine and especially in drug/gene delivery. Diatoms with their unique architectures, good thermal stability, suitable surface area, simple chemical functionalization/modification procedures, ease of genetic manipulations, optical/photonic characteristics, mechanical resistance, and eco-friendliness, can be utilized as smart delivery platforms. The micro- to nanoscale properties of the diatom frustules have garnered a great deal of attention for their application in diverse areas of nanotechnology and biotechnology, such as bioimaging/biosensing, biosensors, drug/gene delivery, photodynamic therapy, microfluidics, biophotonics, solar cells, and molecular filtrations. Additionally, the genetically engineered diatom microalgae-derived nanoporous biosilica have enabled the targeted anticancer drug delivery to neuroblastoma and B-lymphoma cells as well as the mouse xenograft model of neuroblastoma. In this perspective, current trends and recent advances related to the applications of diatoms for the synthesis of nanoparticles, gene/drug delivery, biosensing determinations, biofuel production, and remediation of heavy metals are deliberated, including the underlying significant challenges and future perspectives.

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Section: Applications Of Diatomsmentioning

confidence: 99%

Section: Applications Of Diatomsmentioning

confidence: 99%

Diatoms with Invaluable Applications in Nanotechnology, Biotechnology, and Biomedicine: Recent Advances

Rabiee

Khatami

Soufi

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…verified the viability of biodiesel production from hybrids (karanja and castor oil) using bio‐support spheres immobilized with Pseudomonas cepacia lipase and the reaction using the temperature of 50 ± 1 °C showed the highest yield. Saranya and Ramachandra studied the use of Cladosporium tenuissimum lipase to obtain biodiesel and the maximum lipid activity was at 60 °C and pH 6.00 46 …”

Section: Resultsmentioning

confidence: 99%

“…Saranya and Ramachandra studied the use of Cladosporium tenuissimum lipase to obtain biodiesel and the maximum lipid activity was at 60 °C and pH 6.00. 46 The temperature and pH of the best CRL enzymatic activity depend on the support on which this enzyme is immobilized. Chiou and Wu immobilized CRL in chitosan and lipase showed considerable enzymatic activity at 30 °C and pH 9.00.…”

Section: Enzymatic Activity In the Transesterification Processmentioning

confidence: 99%

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Rial

Freitas

Cavalheiro

et al. 2021

Biofuels Bioprod Bioref

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This work investigated the best reaction conditions to obtain biodiesel from soybean oil using Candida rugosa lipase (CRL) immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel) support. The alcohols used were methanol and ethanol and the molar ratio used was 1:4 (n/n). The CRL immobilized on NBXCel support showed greater catalytic activity at pH 7.00 and at 45 °C. Thus, 23 experiments were carried out at this pH and at this temperature, varying the reaction time, type of alcohol, and amount of immobilized CRL to achieve a conversion equal to or greater than 96.5% as recommended by EN 14103. The highest conversions of methyl biodiesel were achieved using 4.31%mCRL/mNBXCel and with 3.89%mCRL/mNBXCel. Ethyl biodiesel was achieved at the highest conversion using 5.18% and 4.76%mCRL/mNBXCel. The results were explored with regression analysis to identify the best reaction conditions to obtain a good conversion, and the response surface graphs were plotted to visualize other reaction conditions. We also evaluated the number of reuse cycles of CRL immobilized on NBXCel support, to lower biodiesel production cost, and after 15 cycles of reuse the conversions obtained were above 50%, demonstrating the efficiency of CRL immobilized on the NBXCel support in catalyzing the synthesis of biodiesel. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Over the last decade, immense efforts have been made to focus on lipase enzyme synthesis and characterization for a variety of applications. Lipase as a biocatalyst in biodiesel processing is a relatively new eld of research, with speci c applications for rst-and second-generation biodiesel feedstocks including sun ower oil, Jatropha oil, soybean oil, and palm oil (Saranya et al 2020). Enzymatic catalysts can be useful in the synthesis of ethyl esters, but the reaction conditions must be optimized (temperature, molar ratio, pH, amount of catalyst).…”

Section: Use Of Immobilized Lipase In Biodiesel Productionmentioning

confidence: 99%

Green valorization of waste date seed oil for biodiesel production

Mahmoud

Zaki

Ismail

et al. 2022

Preprint

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During recent decades, focus has increased on biodiesel production from wastes or other nonedible resources. The high expense of the manufacturing process is one of the major obstacles it faces. So, continuous research for cheaper and safer resources is more important. Seed oil from various vegetables and fruit species has garnered a lot of: attention recently in the production of biodiesel, such as date palm. The date palm is. one of the most. popular fruits in the Middle East Egypt has recorded now as the highest countries production of dates. The oil of date seeds (DSO) has high concentrations of fatty acids and minerals. In biodiesel production, lipid extraction is an important step. But the advanced extraction techniques are preferred to protect human health and the environment. Therefore, the demand for innovative and environmentally friendly products is now necessary. Transesterification is the process in which oils (mostly triglycerides) bond with alcohol in the existence of a catalyst to form an alkyl ester. Synthesizing the green catalyst and utilizing it in the manufacture of biodiesel have a great added value. The high yield of biodiesel supports the catalyst suitability for the transesterification reaction. Immobilized lipase, moreover, plays a critical function in the transesterification process, that it is more effective and safer. DSO is being studied to see if its fatty acid profile or physicochemical properties suggest it could be a suitable biodiesel source.

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Novel biocatalyst for optimal biodiesel production from diatoms

Cited by 32 publications

References 95 publications

Diatoms with Invaluable Applications in Nanotechnology, Biotechnology, and Biomedicine: Recent Advances

Diatoms with Invaluable Applications in Nanotechnology, Biotechnology, and Biomedicine: Recent Advances

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Green valorization of waste date seed oil for biodiesel production

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