Novel biodegradable sandwich-structured nanofibrous drug-eluting membranes for repair of infected wounds: an in vitro and in vivo study

Chen, Dave Wei-Chih; Liao, Junyi; Liu, Shih‐Jung; Chan, Err–Cheng

doi:10.2147/ijn.s29119

Cited by 34 publications

(15 citation statements)

References 28 publications

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“…In particular, dosage forms for transdermal or topical delivery where release is unidirectional and not isotropic may exhibit release profiles in vitro that are not recapitulated in tissues and cells. For example, Chen et al observed much lower in vivo release of vancomycin, gentamicin and lidocaine compared to in vitro release profiles from poly(lactic-co-glycolic acid) (PLGA)/collagen sandwich-structured nanofibers for topical delivery of wound healing agents (Chen et al , 2012). …”

Section: Introductionmentioning

confidence: 99%

In vitro–ex vivo correlations between a cell-laden hydrogel and mucosal tissue for screening composite delivery systems

et al. 2017

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Composite delivery systems where drugs are electrospun in different layers and vary the drug stacking-order are posited to affect bioavailability. We evaluated how the formulation characteristics of both burst- and sustained-release electrospun fibers containing three physicochemically diverse drugs: dapivirine (DPV), maraviroc (MVC) and tenofovir (TFV) affect in vitro and ex vivo release. We developed a poly(hydroxyethyl methacrylate) (pHEMA) hydrogel release platform for the rapid, inexpensive in vitro evaluation of burst- and sustained-release topical or dermal drug delivery systems with varying microarchitecture. We investigated properties of the hydrogel that could recapitulate ex vivo release into nonhuman primate vaginal tissue. Using a DMSO extraction protocol and HPLC analysis, we achieved >93% recovery from the hydrogels and >88% recovery from tissue explants for all three drugs. We found that DPV loading, but not stacking order (layers of fiber containing a single drug) or microarchitecture (layers with isolated drug compared to all drugs in the same layer) impacted the burst release in vitro and ex vivo. Our burst-release formulations showed a correlation for DPV accumulation between the hydrogel and tissue (R2=0.80), but the correlation was not significant for MVC or TFV. For the sustained release formulations, the PLGA/PCL content did not affect TFV release in vitro or ex vivo. Incorporation of cells into the hydrogel matrix improved the correlation between hydrogel and tissue explant release for TFV. We expect that this hydrogel tissue mimic maybe a promising preclinical model to evaluate topical or transdermal drug delivery systems with complex microarchitectures.

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Section: Introductionmentioning

confidence: 99%

In vitro–ex vivo correlations between a cell-laden hydrogel and mucosal tissue for screening composite delivery systems

et al. 2017

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“…Additionally, a group evaluating the delivery of antibiotics from PLGA scaffolds demonstrated differences in release kinetics in vivo compared to in vitro . 8, 42 However, while they observed these differences, they were still able to obtain sustained release of their drug in vivo , making it a successful delivery method for their application. In our study, it is possible that IBP itself, as well as the addition of a nanofiber scaffold form, causes a distinct change in release in these different environments with the outcome of an unfavorable in vivo release profile.…”

Section: Discussionmentioning

confidence: 99%

Electrospun PLGA Nanofiber Scaffolds Release Ibuprofen Faster and Degrade Slower After In Vivo Implantation

Riggin

Kim

et al. 2017

Ann Biomed Eng

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While delayed delivery of non-steroidal anti-inflammatory drugs (NSAIDs) has been associated with improved tendon healing, early delivery has been associated with impaired healing. Therefore, NSAID use is appropriate only if the dose, timing, and mode of delivery relieves pain but does not impede tissue repair. Because delivery parameters can be controlled using drug-eluting nanofibrous scaffolds, our objective was to develop a scaffold for local controlled release of ibuprofen, and characterize the release profile and degradation both in vitro and in vivo. We found that when incubated in vitro in saline, scaffolds containing ibuprofen had a linear release profile. However, when implanted subcutaneously in vivo or when incubated in vitro in serum, scaffolds showed a rapid burst release. These data demonstrate that scaffold properties are dependent on the environment in which they are placed and the importance of using serum, rather than saline, for initial in vitro evaluation of biofactor release from biodegradable scaffolds.

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“…In general, although these other types of composite materials can exhibit some physical and biological benefits, they typically lack sufficient resistance to suture pull-out and strength, unlike the properties exhibited by our dense chitosan membranes (i.e., ~ 1.4 N for suture pull-out and ~ 86 MPa in maximum stress) [53]. …”

Section: Discussionmentioning

confidence: 99%

Dense chitosan surgical membranes produced by a coincident compression-dehydration process

Dooley

Ellis

Belousova

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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High density chitosan membranes were produced via a novel manufacturing process for use as implantable resorbable surgical membranes. The innovative method utilizes the following three sequential steps: (1) casting an acidic chitosan solution within a silicon mold, followed by freezing; (2) neutralizing the frozen acidic chitosan solution in alkaline solution to facilitate polymerization; and (3) applying coincident compression-dehydration under a vacuum. Resulting membranes of 0.2 – 0.5 mm thickness have densities as high as 1.6 g/cm3. Inclusion of glycerol prior to the compression-dehydration step provides additional physical and clinical handling benefits. The biomaterials exhibit tensile strength with a maximum load as high as 10.9 N at ~ 2.5 mm width and clinically-relevant resistance to suture pull-out with a maximum load as high as 2.2 N. These physical properties were superior to those of a commercial reconstituted collagen membrane. The dense chitosan membranes have excellent clinical handling characteristics, such as pliability and “memory” when wet. They are semi-permeable to small molecules, biodegradable in vitro in lysozyme solution, and the rates of degradation are inversely correlated to the degree of deacetylation. Furthermore, the dense chitosan membranes are biocompatible and resorbable in vivo as demonstrated in a rat oral wound healing model. The unique combination of physical, in vitro, in vivo, and clinical handling properties demonstrate the high utility of dense chitosan membranes produced by this new method. The materials may be useful as surgical barrier membranes, scaffolds for tissue engineering, wound dressings, and as delivery devices for active ingredients.

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Novel biodegradable sandwich-structured nanofibrous drug-eluting membranes for repair of infected wounds: an in vitro and in vivo study

Cited by 34 publications

References 28 publications

In vitro–ex vivo correlations between a cell-laden hydrogel and mucosal tissue for screening composite delivery systems

In vitro–ex vivo correlations between a cell-laden hydrogel and mucosal tissue for screening composite delivery systems

Electrospun PLGA Nanofiber Scaffolds Release Ibuprofen Faster and Degrade Slower After In Vivo Implantation

Dense chitosan surgical membranes produced by a coincident compression-dehydration process

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