Novel biomarkers for the early prediction of pediatric cystic echinococcosis post-surgical outcomes

Salah, Eya Ben; Barrera, Coralie; Sakly, Wahiba; Mosbahi, Sana; Franche, Nathalie; Gottstein, Bruno; Youssef, Sabrine Ben; Mekki, Mongi; Babba, Hamouda; Millon, Laurence

doi:10.1016/j.jinf.2021.09.023

Cited by 6 publications

(1 citation statement)

References 31 publications

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“…Due to the possibility of cyst rupture during surgery, the possibility of forming secondary cysts and recurrence of the disease, follow-up of patients with CE at regular intervals after surgery is essential [ 22 ]. Post-surgical follow up of CE is usually uses ultrasonography and serological methods [ 23 , 24 ]. Ultrasonography which is usually used for post follow up of liver cyst surgery is not effective in lungs CE [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomics investigation of human sera for determination of postoperative indicators of pulmonary cystic echinococcosis

Sadjjadi

Hajjaran

Sedaghat

et al. 2023

J Cardiothorac Surg

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Background Cystic echinococcosis (CE)/hydatidosis is an important zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. The disease is a major health problem all over the world. Finding specific and sensitive biomarkers for follow-up of CE in patients after surgery is essential. Using proteomics methods, the present study aimed to evaluate post-surgical treatment by finding probable biomarker/s in the serum of human lungs CE. Methods A total of 24 human sera were tested. These sera included eight confirmed lung/s CE patients sera before surgery (BS), eight sera 12 months post-surgery (12MPS) as well as eight control sera from healthy people. Proteomics methods including 2DE and LC–MS/MS were performed on the specimens followed by bioinformatics analysis. Differentially expressed proteins (DEP) were detected and, separately integrated with protein–protein interaction (PPI) data to construct the PPI network. Results A total of 171 protein spots were detected in three groups including BS, 12MPS, and control groups; of which a total of 106 DEP have been expressed based on fold changes > = 2 and p-value < 0.05. More analysis was performed and a total of 10 protein spots were selected for identification by mass spectrometry showing the following proteins: APOA1, BGN, SPP2, EAF1, ACOXL, MRPL55, MCTP2, SEPTIN1, B4GALNT1, and ZNF843. Based on centrality parameters of the PPI network (degree and betweenness) five Hub-bottlenecks proteins with significant centrality values were found including APOA1, BGN, SPP2, EAF1, and ACOXL. Conclusion This study showed five proteins as hub-bottleneck proteins; of which APOA1 was more prominent. It can be concluded that a change in expression of this protein in patients’ sera could be used as an indicator tool for the achievement of lungs CE surgical therapy.

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