Novel biotechnological strategies to combat biotic stresses: polygalacturonase inhibitor (PGIP) proteins as a promising comprehensive option

Rathinam, Maniraj; Rao, Uma; Sreevathsa, Rohini

doi:10.1007/s00253-020-10396-3

Cited by 26 publications

(16 citation statements)

References 60 publications

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“…CAZymes mediate the degradation of cellulose, hemicellulose and pectin in host cell walls and break down the physical barrier of plant immune system, and thus enhancing pathogen virulence (Zheng et al 2013 ; Xia et al 2017 ). CWDEs in R. solani are categorized into seven major families including PG, endo-β- 1,4-glucanase, polymethylgalacturonase, pectin methylesterase, filter paper enzyme, polymethylgalacturonase trans-eliminas and pectin methyltrans-eliminase (Silva et al 2018 ; Xue et al 2018 ; Rathinam et al 2020 ). As a group of hydrolytic enzymes, PGs act on polygalacturonic acid, a key component of plant cell wall, by cleaving the α-(1,4)-glycosidic bonds, and thus causing hydrolysis of pectate.…”

Section: Pathogenicity Factors In R Solanimentioning

confidence: 99%

“…As a countermeasure to fungal PGs, plant polygalacturonase-inhibiting proteins (PGIPs) specifically recognize and bind to fungal PGs, and thereby inhibit the cell wall degradation activity and prevent fungal infection (Rathinam et al 2020 ). The interaction of PGs and PGIPs results in the production of oligogalacturonides, which are important signals to induce PGIP gene expression and plant defense responses (Davidsson et al 2017 ).…”

Section: Countermeasures From the Hostmentioning

confidence: 99%

“…The attempts at inhibiting the PG activity via PGIP overexpression are also successful in suppressing R. solani infection. Overexpression of OsPGIP1 significantly improves rice resistance to RSB (Chen et al 2016 ; Rathinam et al 2020 ; Wang et al 2015b ). Although OsPGIP2, a homolog of OsPGIP1, has no inhibitory activity to PGs, the mutant protein OsPGIP2 L233F has been identified to have the PG inhibition activity.…”

Section: Countermeasures From the Hostmentioning

confidence: 99%

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Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Wei

et al. 2021

Rice

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Rhizoctonia solani is an important phytopathogenic fungus with a wide host range and worldwide distribution. The anastomosis group AG1 IA of R. solani has been identified as the predominant causal agent of rice sheath blight, one of the most devastating diseases of crop plants. As a necrotrophic pathogen, R. solani exhibits many characteristics different from biotrophic and hemi-biotrophic pathogens during co-evolutionary interaction with host plants. Various types of secondary metabolites, carbohydrate-active enzymes, secreted proteins and effectors have been revealed to be essential pathogenicity factors in R. solani. Meanwhile, reactive oxygen species, phytohormone signaling, transcription factors and many other defense-associated genes have been identified to contribute to sheath blight resistance in rice. Here, we summarize the recent advances in studies on molecular interactions between rice and R. solani. Based on knowledge of rice-R. solani interactions and sheath blight resistance QTLs, multiple effective strategies have been developed to generate rice cultivars with enhanced sheath blight resistance.

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Section: Pathogenicity Factors In R Solanimentioning

confidence: 99%

Section: Countermeasures From the Hostmentioning

confidence: 99%

Section: Countermeasures From the Hostmentioning

confidence: 99%

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Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Wei

et al. 2021

Rice

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“…PGs are among the first enzymes to be secreted by phytopathogenic microorganisms during colonization (5-7) and have been shown to be important pathogenicity factors in fungi (8)(9)(10)(11) and bacteria (12,13). Plants defend themselves by secreting PG-inhibiting proteins (PGIPs) into their cell wall to counteract microbial PGs (14)(15)(16). Besides restricting the pectin degradation, inhibition of PGs by PGIPs favors the formation of oligogalacturonides (17,18).…”

mentioning

confidence: 99%

Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy

Haeger

Henning

Heckel

et al. 2020

Journal of Biological Chemistry

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Plant cell wall-associated polygalacturonase-inhibiting proteins (PGIPs) are widely distributed in the plant kingdom. They play a crucial role in plant defense against phytopathogens by inhibiting microbial polygalacturonases (PGs). PGs hydrolyze the cell wall polysaccharide pectin and are among the first enzymes to be secreted during plant infection. Recent studies demonstrated that herbivorous insects express their own PG multi-gene families, raising the question whether PGIPs also inhibit insect PGs and protect plants from herbivores. Preliminary evidence suggested that PGIPs may negatively influence larval growth of the leaf beetle Phaedon cochleariae (Coleoptera: Chrysomelidae) and identified BrPGIP3 from Chinese cabbage (Brassica rapa ssp. pekinensis) as a candidate. PGIPs are predominantly studied in planta because their heterologous expression in microbial systems is problematic and instability and aggregation of recombinant PGIPs has complicated in vitro inhibition assays. To minimize aggregate formation, we heterologously expressed BrPGIP3 fused to a glycosylphosphatidylinositol (GPI) membrane anchor, immobilizing it on the extracellular surface of insect cells. We demonstrated that BrPGIP3_GPI inhibited several P. cochleariae PGs in vitro, providing the first direct evidence of an interaction between a plant PGIP and an animal PG. Thus, plant PGIPs not only confer resistance against phytopathogens, but may also aid in defense against herbivorous beetles.

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“…PGIP представляют собой гликопротеины, структурно близкие обширной группе богатых лейциновыми повторами (Leucine-repeat rich, LRR) белков, вовлеченных в реализацию важнейших механизмов иммунитета растений к бактериям, грибам и насекомым (Yarullina et al, 2016;Rathinam et al, 2020).…”

Section: полигалактуроназыunclassified

Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

Konarev¹

2020

PPN

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Novel biotechnological strategies to combat biotic stresses: polygalacturonase inhibitor (PGIP) proteins as a promising comprehensive option

Cited by 26 publications

References 60 publications

Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy

Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

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