Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Abstract

The objective of this experiment was to identify the novel single nucleotide polymorphisms (SNPs) on porcine follicle stimulating hormone  subunit (FSH) genes. Moreover, their association with litter size traits in commercial pigs will be analyzed. 1,155 Large White x Landrace crossbred sows were bled and DNA was extracted. The records included total number of piglet born (TNB), number of piglet born alive (NBA), number of piglets stillbirth (SB) and number of piglets mummified (MM). The known sequence of porcine FSH (GenBank accession no. D00621.1) was screened homology to known sequences in term of express sequenced tags (ESTs) in public domain, GenBank. The primers were designed for amplified the novel BsuRI-c.930A>G-FSH fragment which confirmed by PCR-RFLP and nucleotide sequencing then genotyping. The favorable cut homozygous G/G allele was highly significant higher than A/G allele in terms of TNB and NBA. While, published marker HaeIII-g.5894A>G-FSH was not significant difference in any litter traits. For haplotype analysis, c.930A>G-g.5894A>G of FSHβ, unfavorable GG/AA haplotype was significantly lower TNB and NBA than other haplotypes. Conversely, this haplotype was significantly higher MM than others. The study concluded that BsuRI-c.930A>G and the haplotype of HaeIII-g.5894A>G -BsuRI-c.930A>G of FSH may used for marker-assisted selection on pig breeding program.

Polymorphism of candidate genes associated with reproductive performance in crossbred pigs

“…FSHβ (c.930A>G) SNP: The length of amplified PCR product (930 bp) was similar to the findings of Pripwai and Mekchay (2011). Monomorphism at this SNP locus was however contrary to the findings of Pripwai and Mekchay (2011) in crossbred pigs of Large White and Landrace breeds. They reported that frequency of A and G allele at FSHâ (c.930A>G) SNP locus was 0.81 and 0.19, respectively.…”

“…Niu et al (2006) studied the polymorphism of Prei3 (T802G) in seven pig breeds and suggested that pre-implantation protein 3 (prei3) is one of the promising candidate gene for litter size in pigs. Pripwai and Mekchay (2011) identified a novel SNP at BsuRIc 930A>G in porcine follicle stimulating hormone (FSHâ) gene having significant association with litter size in commercial pigs. Kumchoo and Mekchay (2015) identified SNP at c.425G>A in OPN gene having significant association with litter size in commercial Thai Large White pigs.…”