Novel calcitonin gene‐related peptide/chitosan‐strontium‐calcium phosphate cement: Enhanced proliferation of human umbilical vein endothelial cells <i>in vitro</i>

Lv, Tiancheng; Liang, Wen‐Miin; Li, Li; Cui, Xu; Wei, Xiaomou; Pan, Haobo; Li, Bing

doi:10.1002/jbm.b.34091

Cited by 22 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of chitosan may be beneficial for anti‐washout. Aqueous solutions of chitosan are high in viscosity, and the viscosity will be increased by the cross‐linking of citric acid, which enhances the anti‐washed of the specimen 34,35 . Moreover, the cements also showed good injectability, indicating that the materials were beneficial for minimally invasive surgery.…”

Section: Discussionmentioning

confidence: 99%

Cu‐loaded Brushite bone cements with good antibacterial activity and operability

Zhang

et al. 2020

J Biomed Mater Res

View full text Add to dashboard Cite

Bone defect‐related surgical procedures are traumatic processes carrying potential inflammation and infection risks in the clinic, which are associated with prolonged antibiotic therapy that promotes bacterial antibiotic‐resistance. In the present study, Cu‐loaded brushite bone cements were designed, and the properties of the bone cements were evaluated. The setting time of the cement was prolonged from 12 to 50 min as the copper content increased. All cements were anti‐washout, and the injectable coefficient of the cements was approximately 88%. Scanning electron microscopy results revealed that the crystal grains grew larger and thicker as the copper content in the cement increased, and brushite was determined to be the dominant crystalline phase for all the cements. However, a small amount of newly formed calcium copper phosphate was observed in the cement. Simultaneously, band shifts were observed in the Fourier transform infrared spectroscopy results at a Cu content of 5%. Moreover, the addition of Cu improved the compressive strength of brushite cements, and all cements were degradable. Furthermore, the Cu‐loaded brushite bone cements performed well in inhibiting the growth and proliferation of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, and the diameter of the inhibition zone increased with increasing copper content. The study revealed that the Cu‐loaded brushite bone cements possessed good cellular affinity to mouse bone marrow stem cells when a lower dose of copper was added in vitro. These results support the great potential of injectable antibacterial brushite bone cement specifically for bone tissue defect‐related repair and regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Cu‐loaded Brushite bone cements with good antibacterial activity and operability

Zhang

et al. 2020

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…In addition, novel calcitonin gene-related peptide/chitosan-strontium-calcium phosphate cement enhances the proliferation of HUVECs (41). Furthermore, increasing extracellular Ca 2+ significantly triggers TNF-α-induced vascular cell adhesion molecule 1 activation and monocyte adhesion in HUVECs (42). Several reports have revealed that the p38 MAPK inhibitor (CBS3830) restricts vascular re-modeling in arterialized vein grafts (35).…”

Section: Discussionmentioning

confidence: 99%

Expression profile of tRNA‑derived fragments and their potential roles in human varicose veins

Wang

Yun

et al. 2019

Mol Med Report

View full text Add to dashboard Cite

Varicose veins (VVs) is a common disease presenting with chronic venous insufficiency. tRNA-derived fragments (tRFs) are associated with a variety of pathological conditions. However, the functions of tRFs in VVs have not been elucidated to date. The present study aimed to identify the key tRFs and investigate their potential roles in VVs. Small RNA sequencing (RNA-seq) was performed to investigate the expression of tRFs in tissues of patients with VVs and their matched adjacent normal veins tissues (ANVs). Reverse transcription-quantitative PCR (RT-qPCR) was used to confirm the differential expression of tRFs. A total of 13,789 tRFs were identified by small RNA-seq, including 45 differentially expressed tRFs (DETs), which comprised 14 upregulated and 31 downregulated tRFs in VV tissues compared with ANVs. In addition, DETs were mainly involved in the function of epidermal growth factor receptor and vascular endothelial growth factor receptor signaling pathways in VVs. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the target genes of DETs were predominantly involved in Wnt and mitogen-activated protein kinase (MAPK) signaling pathways, as well as calcium signaling. Additionally, two upregulated tRFs (tRF-36-F900BY4D84KRIME and tRF-23-87R8WP9IY) and one downregulated tRF (tRF-40-86J8WPMN1E8Y7Z2R) were further validated by RT-qPCR, and a signaling pathway regulation network of their target genes confirmed their involvement in the calcium, Wnt and MAPK signaling pathways. The results of the present study identified three DETs (tRF-36-F900BY4D84KRIME, tRF-23-87R8WP9IY and tRF-40-86J8WPMN1E8Y7Z2R), which may have crucial roles in the occurrence and progression of VVs by regulating Wnt and MAPK signaling, as well as calcium signaling. The present results may provide a basis for further investigation of the functional roles of tRFs in VVs.

show abstract

“…Rat osteosarcoma cells, bone stromal cells, and chondrocytes attached well to RGD–chitosan and promoted mineralization [ 33 , 53 , 59 ]. Further, Lv et al reported that calcitonin gene-related peptide/chitosan containing cement promoted VEGF (vascular endothelial growth factor) mRNA expression and HUVEC (human umbilical vein endothelial cell) proliferation by controlling the release of the calcitonin gene-related peptide [ 60 ], and SIKVAV–chitosan hydrogels accelerated the re-epithelization of wounds and promoted angiogenesis in vivo [ 61 ].…”

Section: Properties Of Peptide–chitosan Matricesmentioning

confidence: 99%

Mixed Peptide-Conjugated Chitosan Matrices as Multi-Receptor Targeted Cell-Adhesive Scaffolds

Hozumi

Nomizu

2018

IJMS

View full text Add to dashboard Cite

Biomaterials are important for cell and tissue engineering. Chitosan is widely used as a scaffold because it is easily modified using its amino groups, can easily form a matrix, is stable under physiological conditions, and is inactive for cell adhesion. Chitosan is an excellent platform for peptide ligands, especially cell adhesive peptides derived from extracellular matrix (ECM) proteins. ECM proteins, such as collagen, fibronectin, and laminin, are multifunctional and have diverse cell attachment sites. Various cell adhesive peptides have been identified from the ECM proteins, and these are useful to design functional biomaterials. The cell attachment activity of peptides is influenced by the solubility, conformation, and coating efficiency to solid materials, whereas immobilization of peptides to a polysaccharide such as chitosan avoids these problems. Peptide–chitosan matrices promote various biological activities depending on the peptide. When the peptides are immobilized to chitosan, the activity of the peptides is significantly enhanced. Further, mixed peptide–chitosan matrices, conjugated with more than one peptide on a chitosan matrix, interact with multiple cellular receptors and promote specific biological responses via receptor cross-talk. Receptor cross-talk is important for mimicking the biological activity of ECM and the proteins. The mixed peptide–chitosan matrix approach is useful to develop biomaterials as a synthetic ECM for cell and tissue engineering.

show abstract

Novel calcitonin gene‐related peptide/chitosan‐strontium‐calcium phosphate cement: Enhanced proliferation of human umbilical vein endothelial cells in vitro

Cited by 22 publications

References 29 publications

Cu‐loaded Brushite bone cements with good antibacterial activity and operability

Cu‐loaded Brushite bone cements with good antibacterial activity and operability

Expression profile of tRNA‑derived fragments and their potential roles in human varicose veins

Mixed Peptide-Conjugated Chitosan Matrices as Multi-Receptor Targeted Cell-Adhesive Scaffolds

Contact Info

Product

Resources

About