Novel carboxylated PEG-coating on magnetite nanoparticles designed for biomedical applications

Illés, Erzsébet; Tombácz, Etelka; Szekeres, Márta; Tóth, Ildikó Y.; Szabó, Ákos; Iván, Béla

doi:10.1016/j.jmmm.2014.10.146

Cited by 61 publications

(38 citation statements)

References 34 publications

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“…To enhance the binding affinity of PEG-OH to the NPs, we further engineered mPEG using a simple succinylation procedure. mPEG-5000 was chosen, as it is an FDA-approved polymer and its use in biomedical applications has been well-documented [156][157][158][159][160][161][162][163]. Succinylated PEG was produced in-house from the PEG-OH terminal of mPEG in a process, during which the terminal hydroxyl group was converted, by a small chain extension, to a more electronegative carboxyl group.…”

Section: Polyethylene Glycol Succinylation and Cappingmentioning

confidence: 99%

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Armijo¹,

Wawrzyniec²,

Kopciuch³

et al. 2020

J Nanobiotechnol

128

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Background: Novel methods are necessary to reduce morbidity and mortality of patients suffering from infections with Pseudomonas aeruginosa. Being the most common infectious species of the Pseudomonas genus, P. aeruginosa is the primary Gram-negative etiology responsible for nosocomial infections. Due to the ubiquity and high adaptability of this species, an effective universal treatment method for P. aeruginosa infection still eludes investigators, despite the extensive research in this area. Results:We report bacterial inhibition by iron-oxide (nominally magnetite) nanoparticles (NPs) alone, having a mean hydrodynamic diameter of ~ 16 nm, as well as alginate-capped iron-oxide NPs. Alginate capping increased the average hydrodynamic diameter to ~ 230 nm. We also investigated alginate-capped iron-oxide NP-drug conjugates, with a practically unchanged hydrodynamic diameter of ~ 232 nm. Susceptibility and minimum inhibitory concentration (MIC) of the NPs, NP-tobramycin conjugates, and tobramycin alone were determined in the PAO1 bacterial colonies. Investigations into susceptibility using the disk diffusion method were done after 3 days of biofilm growth and after 60 days of growth. MIC of all compounds of interest was determined after 60-days of growth, to ensure thorough establishment of biofilm colonies. Conclusions:Positive inhibition is reported for uncapped and alginate-capped iron-oxide NPs, and the corresponding MICs are presented. We report zero susceptibility to iron-oxide NPs capped with polyethylene glycol, suggesting that the capping agent plays a major role in enabling bactericidal ability in of the nanocomposite. Our findings suggest that the alginate-coated nanocomposites investigated in this study have the potential to overcome the bacterial biofilm barrier. Magnetic field application increases the action, likely via enhanced diffusion of the iron-oxide NPs and NP-drug conjugates through mucin and alginate barriers, which are characteristic of cystic-fibrosis respiratory infections. We demonstrate that iron-oxide NPs coated with alginate, as well as alginate-coated magnetite-tobramycin conjugates inhibit P. aeruginosa growth and biofilm formation in established colonies. We have also determined that susceptibility to tobramycin decreases for longer culture times. However, susceptibility to the iron-oxide NP © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Section: Polyethylene Glycol Succinylation and Cappingmentioning

confidence: 99%

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Armijo¹,

Wawrzyniec²,

Kopciuch³

et al. 2020

J Nanobiotechnol

128

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“…Magnetite nanoparticles were synthesized by alkaline hydrolysis [42][43][44] of FeCl 2 × 4 H 2 O and FeCl 3 × 6 H 2 O. e concentrated solutions of iron (II) and iron (III) salts were mixed in the molar ratio of 1.1 to 2 and filtered into fresh water using a microfilter of 0.2 µm pore size. e calculated amount of freshly prepared NaOH solution was added in 10% excess to the doubly diluted iron salt solution in two portions, the first half slowly and the second half rapidly under rigorous stirring.…”

Section: Synthesis Of Mnpsmentioning

confidence: 99%

A Simple and Scalable Method for the Preparation of Magnetite/Graphene Oxide Nanocomposites under Mild Conditions

Szabó

Nánai

Nesztor

et al. 2018

Advances in Materials Science and Engineering

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Nanostructured composite dispersions containing magnetic nanoparticles (MNPs) and graphene oxide (GO) lamellae have been prepared by a simple and easily scalable room temperature procedure. We show that, owing to the enormous surface area and negative surface charge developed in aqueous GO suspensions, large amounts of positively charged MNPs can be electrostatically attached to the layered host. is procedure is superior to many previous synthesis pathways because it exploits the chargeregulated adhesion of naked MNPs to GO resulting in the formation of stable and uniform nanocomposite materials in a wide composition range without any preliminary functionalization steps or harsh conditions that may lead to chemical degradation of the graphene-based nanosheets.

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“…DNA). Perhaps the most successful core-shell products are carboxylgroup functionalized SPIONs, since they are not only easy to functionalize in a huge variety of reactions of the carboxyl groups, but also provide high stability to SPIONs in a biological milieu by decreasing the isoelectric point (pHiep) of iron oxide nanoparticles [7][8][9][10][11]. However, protein adsorption occurs on the surface of carboxylated SPIONs and the formation of hard/soft protein coronas [12,13] can give rise to cellular recognition of the nanoparticles by phagocytes or inflammatory cells (opsonization), which reduce significantly the efficiency of active targeting and may cause problems in their application in vivo.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application

Illés

Szekeres

Tóth

et al. 2018

Journal of Magnetism and Magnetic Materials

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Highlights• Multicore magnetite nanoparticles (MNPs) were superparamagnetic.• PEG-carboxylate polyelectrolytes coat spontaneously MNPs and stabilize them electrosterically.• Biofunction can be attached to MNPs via carboxylated coating layer.• Multifunctional shell prevents MNPs' internalization into cells.• Superparamagnetic property is sustained after MNP coating. AbstractBiocompatible magnetite nanoparticles (MNPs) were prepared by post-coating the magnetic nanocores with a synthetic polymer designed specifically to shield the particles from nonspecific interaction with cells. Poly(ethylene glycol) methyl ether methacrylate (PEGMA) macromonomers and acrylic acid (AA) small molecular monomers were chemically coupled by quasi-living atom transfer radical polymerization (ATRP) to a comb-like copolymer, P(PEGMA-co-AA) designated here as P(PEGMA-AA). The polymer contains pendant carboxylate moieties near the backbone and PEG side chains. It is able to bind spontaneously to MNPs; stabilize the particles electrostatically via the carboxylate moieties and sterically via the PEG moieties; provide high protein repellency via the structured PEG layer; and anchor bioactive proteins via peptide bond formation with the free carboxylate groups. The presence of the P(PEGMA-AA) coating was verified in XPS experiments. The electrosteric (i.e., combined electrostatic and steric) stabilization is efficient down to pH 4 (at 10 mM ionic strength). Static magnetization and AC susceptibility measurements showed that the P(PEGMA-AA)@MNPs are superparamagnetic with a saturation magnetization value of 55 emu/g and that both single core nanoparticles and multicore structures are present in the samples. The multicore components make our product well suited for magnetic hyperthermia applications (SAR values up to 17.44 W/g). In vitro biocompatibility, cell internalization, and magnetic hyperthermia studies demonstrate the excellent theranostic potential of our product. Graphical abstract

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Novel carboxylated PEG-coating on magnetite nanoparticles designed for biomedical applications

Cited by 61 publications

References 34 publications

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

A Simple and Scalable Method for the Preparation of Magnetite/Graphene Oxide Nanocomposites under Mild Conditions

Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application

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