Novel carboxymethyl chitosan-graphene oxide hybrid particles for drug delivery

Abstract

We describe an electrostatic droplet generation method to prepare a novel carboxymethyl chitosan-graphene oxide hybrid particles for delivery purpose. Under an adjustable electrostatic field, graphene oxide and carboxymethyl chitosan mixed solution was sprayed as uniform micro-droplets, which were solidified as particles in CaCl solution. Such hybrid particles are wished to have excellent stability in saline solution, and better delivery properties than pristine carboxymethyl chitosan particles. The effects of micro-droplets generation conditions on particles formation were systematically investigated. At conditions of 40 mg/ml of carboxymethyl chitosan, 2 mg/ml of graphene oxide, 3 ml/h of feed speed, electrostatic field parameters was 9 kV and 20 cm, uniformly sized carboxymethyl chitosan-graphene oxide particles in the diameter range of 250-300 μm was successfully prepared. In NaCl saline, these particles could maintain stable for at least a week, while pristine carboxymethyl chitosan particles quickly collapsed within an hour. The results of loading experiments showed that carboxymethyl chitosan-graphene oxide particles could effectively adsorb gatifloxacin, ofloxacin, bovine serum albumin, lysozyme, doxorubicin hydrochloride. And gatifloxacin was chosen as a model drug to study the exact effect of graphene oxide content on the loading and release properties. In 40:2 group, the highest loading capacity of 0.45 ± 0.19 mg/mg was achieved, and also a good sustained release was available. Above all, we believed that carboxymethyl chitosan-graphene oxide particles as a versatile carrier, has great potential in Medicine and Pharmacy.

Effects of aspirin-loaded graphene oxide coating of a titanium surface on proliferation and osteogenic differentiation of MC3T3-E1 cells

“…Regarding the drug release from A/Ti and A/Ti-GO in PBS, the initial burst of aspirin release from the surface of Ti happened in the first 0.5 h, but this did not occur for Ti-GO. Because of the interaction between the aspirin benzene ring and functional groups on Ti-GO 25 (which controlled the aspirin release), the duration of aspirin release was more than 72 h. This indicates that a GO coating on a Ti substrate is a promising approach for drug delivery. We can expect that it will provide a cost-effective method of implant material modification that will be highly useful in the early stage after implantation.…”

“…In recent years, graphene oxide (GO) has attracted considerable interest in terms of its biomedical applications such as in cancer therapy 23 , tissue engineering 24 , and drug and gene delivery 25 , 26 on account of its unique physical, chemical, and mechanical properties 27 , 28 . GO consists of single sheets of sp 2 -hybridized carbon (C) atoms arranged within honeycomb lattices.…”

Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

“…Chitosan has been investigated extensively for drug delivery systems. 15 The chitosan-gelatin scaffold prepared by the freeze-gelation method provides better conditions for NP cell proliferation. 16 The advantage of ECM molecules is that it allows cells to maintain their differentiated phenotype for specific tissues.…”

Sub-Acute Toxicity Study of Graphene Oxide in the Sprague-Dawley Rat

“…GO has high surface area and a strong adsorption capacity for drugs and maintains drug stability without altering biological activity, far more than other nanomaterials [10,11,12]. Studies show that GO with lateral sizes from 200 μm to 500 nm can serve as a means of drug delivery [13,14]. …”