Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells

Hida, Naoko; Nishiyama, Nobuhiro; Miyoshi, Shunichiro; Kira, Shintaro; Segawa, Kaoru; Uyama, Taro; Mori, Taisuke; Miyado, Kenji; Ikegami, Yasuyuki; Cui, Chang Hao; Kiyono, Tohru; Kyo, Satoru; Shimizu, Tatsuya; Okano, Teruo; Sakamoto, Michiie; Ogawa, Satoshi; Umezawa, Akihiro

doi:10.1634/stemcells.2007-0826

Cited by 295 publications

(280 citation statements)

References 28 publications

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“…Nevertheless, recent evidences suggest that adult progenitor cells can be used to fabricate ex vivo engineered cardiac tissue to be implanted in the injured myocardium. To this aim, progenitor cells can be appropriately grown on polymeric scaffolds [2,3] or, alternatively, thermo-responsive surfaces [4][5][6]. Following the latter procedure, scaffoldless tissue sheets fabricated using clonally expanded stem cell antigen-1 positive (Sca-1 pos ) progenitor cells (CPCs) isolated from murine hearts, prevascularized [7] and transplanted into hearts with experimental myocardial infarction have shown to prevent cardiac Author contributions: G.F. and S.P.…”

Section: Introductionmentioning

confidence: 99%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.

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Section: Introductionmentioning

confidence: 99%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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“…Cell culture: Human endometrial-derived mesenchymal cells (EMCs) [14] were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich Japan, Tokyo) supplemented with 10% fetal bovine serum (FBS) (Japan Bio Serum, Nagoya), 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA) at 37 o C in a humidified incubator with 5% CO 2 . EMCs showed adherent spindle shape morphology.…”

Section: Temperature-responsive Culture Dishesmentioning

confidence: 99%

Thickness limitation and cell viability of multi-layered cell sheets and overcoming the diffusion limit by a porous-membrane culture insert

Sekine¹,

Haraguchi²

2015

J Biochip Tissue chip

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A major challenge of tissue engineering is the creation of three-dimensional (3D) and functional tissues. However, the tissue ischemic environments make the production of thicker 3D tissues difficult. For evaluating the thickness limitation of tissue, the cell viability and metabolism of a novel in vitro 3D tissue model, which is composed of multilayered cell sheets, were investigated. Human endometrial-derived mesenchymal cells (EMC) were cultured on temperature-responsive culture dishes. Confluently cultured EMCs were harvested as an intact contiguous cell sheet only by lowering temperature. The obtained cell sheets were successfully layered into 3D cell-dense tissues and cultured in vitro for 7 days. Glucose consumption and lactate production in the culture media increased in accordance with the number of layers (single to triple). Histological analyses and cell viability assays showed that viable tissues were found in single-to triple-layered cell sheets and damaged tissues in over quadruple layers. The results concluded that the thickness limitation of layered cell sheets was approximately 40 mm, which was the thickness of triple-layered cell sheets. Importantly, when multi-layered cell sheets were cultured on porous membranes, the adhesion among cell sheets and cell viability were improved, resulting in successful fabricating thicker tissues (~100 mm) than that on normal culture dishes. The metabolic analyses showed that multi-layered cell sheets rely on their anaerobic metabolism, indicating that supplying nutrients rather than oxygen through both upper and bottom tissue surfaces improved the cell viability in 3D tissues. ` `Citation: Sekine W, Haraguchi Y, Shimizu T, Umezawa A, Okano T (2011) Thickness limitation and cell viability of multi-layered cell sheets and overcoming the diffusion limit by a porous-membrane culture insert. J Biochip Tissue chip S1:007.

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“…Клітини раку, в свою чергу, експресують широкий спектр рецепторів до цих речовин, які регулюють клітинний і гумора-льний імунітет і відіграють важливу роль у визна-ченні напрямку розвитку пухлини [16,17]. Цю сис-тему злоякісні клітини використовують для парак-ринного стимулювання і метастазування [18,19], за-лучення клітин імунної системи [19]. Вплив цитокі-нів на клітини здійснюється через різні молекулярні шляхи.…”

Section: літературний оглядunclassified

“…Маркерними білками для раку молочної зало-зи (РМЗ), що здійснюють передачу гуморальних сиг-налів в клітину є естрогенові рецептори (ЕР) і рецеп-тори епідермального фактора росту (р-ЕФР) [20][21][22]. Рівень експресії ЕР і р-ЕФР корелює з проліфератив-ної і метастазуючою активністю пухлинних клітин [19,[23][24][25], а також з чутливістю клітин РМЗ до про-типухлинних препаратів [16,26,27]. …”

Section: літературний оглядunclassified

Influence of mesenchymal stem cells on tumor cells of breast cancer in non-contact co-cultivation

et al. 2015

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Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells

Cited by 295 publications

References 28 publications

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

Thickness limitation and cell viability of multi-layered cell sheets and overcoming the diffusion limit by a porous-membrane culture insert

Influence of mesenchymal stem cells on tumor cells of breast cancer in non-contact co-cultivation

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