Novel casein-derived peptides with antihypertensive activity

Contreras, María del Mar; Carrón, Rosalı́a; Montero, María; Ramos, Mercedes; Recio, Isidra

doi:10.1016/j.idairyj.2009.05.004

Cited by 218 publications

(106 citation statements)

References 50 publications

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“…These results were similar to Silva et al (2012), Contreras et al (2009) andAlli et al (1998) when investigated the peptide profile of bovine casein in "coalho" cheese, ovine casein and bovine casein from cheddar cheese, respectively.…”

Section: Antioxidant Activity Evaluation Of Caprine Casein Hydrolysatessupporting

confidence: 87%

Biotechnological richness of the northeastern semi-arid region: antioxidant activity of casein hydrolysates from Moxotó goat milk (Capra hircus Linnaeus, 1758) obtained by papain action

et al. 2013

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Section: Antioxidant Activity Evaluation Of Caprine Casein Hydrolysatessupporting

confidence: 87%

Biotechnological richness of the northeastern semi-arid region: antioxidant activity of casein hydrolysates from Moxotó goat milk (Capra hircus Linnaeus, 1758) obtained by papain action

et al. 2013

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“…Prior to QSAR modeling, proteins have to be enzymatically hydrolyzed and isolated using bioassay guided fractionation; the peptides are then identified by MS/MS spectrometry and subsequently synthesized for structure-activity relationship. Several studies have been conducted using this model of rats to screen potential antihypertensive peptides from different food sources (Cheng et al, 2008;Contreras, Carrón, Montero, Ramos & Recio, 2009;Girgih, Udenigwe, Hasan, Gill & Aluko, 2013;Hiwatashi et al, 2010;Lee, Qian & Kim, 2010;Miguel, Gómez-Ruiz, Recio & Aleixandre, 2010;Quiñones et al, 2010;Staljanssens et al, 2011;. The antihypertensive properties of peptides have recently been reviewed and two main routes (oral gavage and intravenous administration) are employed for administering these peptides to the rats.…”

Section: Structure-function Relationship Of Bioactive Peptidesmentioning

confidence: 99%

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Girgih

Malomo

et al. 2014

Journal of Functional Foods

241

142

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The aim of this work was to produce enzymatic hemp seed protein hydrolysates (HPH) followed by bioassay guided fractionation to identify antioxidant and antihypertensive peptides. Therefore, simulated gastrointestinal digestion of isolated hemp seed proteins was conducted using consecutive actions of pepsin and pancreatin to produce HPH, which was then separated by membrane ultrafiltration to obtain peptide sizes of <1, 1-3, 3-5, and 5-10 kDa. Evaluation of HPH and its membrane fractions for antioxidant and antihypertensive properties showed that they significantly (P<0.05) scavenged radicals, reduced and strongly chelated metal ions as well as inhibited lipid oxidation. During a 24-hr test, the HPH reduced systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR) after oral administration by a maximum of -30 mmHg when compared to -15 mmHg for the membrane fractions.To reduce production cost, hemp seed protein meal (HPM) was directly hydrolyzed to a protein hydrolysate (HMH) and was shown to also reduce SBP during 4-8 weeks of dietary feeding. The attenuation of SBP correlated to suppressed plasma levels (0.047-0.059 U/mL and 0.040-0.054 µg/mL) of angiotensin converting enzyme (ACE) and renin, respectively, when compared to the control rats (0.123 U/mL and 0.151 µg/mL). A total of 23 peptides were identified to be present in the HPH. WVYY and PSLPA showed superior in vitro antioxidant properties, while ACE activity was inhibited by WYT (89%), WVYY (91%) and PSLPA (90%). Renin activity was inhibited by WYT (77%), SVYT (87%) and IPAGV (75%). However, oral administration to SHR showed that the pentapeptides (PSLPA and IPAGV) were more effective SBP-reducing agents (-40 mm Hg) when compared to the tri-(-13 mmHg) and tetrapeptides (-36 mmHg). These results

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“…Within the group of bioactive peptides, the ones that have attracted higher scientific and industrial interest are those having antihypertensive activity. Interest in studying these compounds has arisen from the increase in mortality in industrialized countries due to hypertension and/or renal, heart or brain complications (Rojas-Ronquillo et al, 2012;Contreras et al, 2009;Nielsen et al, 2009;Saito, 2008;Hartmann & Meisel, 2007;Quirós et al, 2005;Wu & Ding, 2001;Takano, 1998).…”

Section: Introductionmentioning

confidence: 99%

Peptide separation of commercial fermented milk during refrigerated storage

González-Olivares¹,

Añorve‐Morga²,

Castañeda‐Ovando³

et al. 2014

Food Sci. Technol (Campinas)

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Milk is an important source of bioactive compounds. Many of these compounds are released during fermentation and refrigerated storage. The aim of this study was to determine the release of peptides by lactic acid bacteria in commercial fermented milk during refrigerated storage. The size and profile of peptides were analyzed by polyacrylamide gel electrophoresis and sizeexclusion HPLC. During electrophoresis, it was observed that the peptides were released from caseins, whereas β-lactoglobulin was the whey protein with the highest degradation. HPLC analysis confirmed the pattern of peptide formation observed in electrophoresis. Two fractions lower than 2 kDa with aromatic amino acids in their structure were separated. These results were consistent with those reported for structures of peptides with antihypertensive activity. Therefore, the presence of aromatic amino acids in the peptide fractions obtained increases the likelihood of finding peptides with such activity in refrigerated commercial fermented milk. In conclusion, during cold storage, peptides with different molecular weights are released and accumulated. This could be due to the action of proteinases and peptidases of the proteolytic system in lactic acid bacteria.

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Novel casein-derived peptides with antihypertensive activity

Cited by 218 publications

References 50 publications

Biotechnological richness of the northeastern semi-arid region: antioxidant activity of casein hydrolysates from Moxotó goat milk (Capra hircus Linnaeus, 1758) obtained by papain action

Biotechnological richness of the northeastern semi-arid region: antioxidant activity of casein hydrolysates from Moxotó goat milk (Capra hircus Linnaeus, 1758) obtained by papain action

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Peptide separation of commercial fermented milk during refrigerated storage

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