Novel Cefotaximase (CTX-M-16) with Increased Catalytic Efficiency Due to Substitution Asp-240→Gly

Bonnet, Richard; Dutour, C.; Sampaio, Jorge Luiz Mello; Chanal, C.; Sirot, D.; Labia, Roger; Champs, Christophe de; Sirot, J.

doi:10.1128/aac.45.8.2269-2275.2001

Cited by 162 publications

(134 citation statements)

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“…Clinical isolates of Proteus mirabilis, C. amalonaticus, E. cloacae and E. aerogenes collected from hospitals in Rio de Janeiro (Brazil) also demonstrated bla CTX-M-2 (Bonnet et al 2000, Bonnet et al 2001. In South America, the bla CTX-M-2 appears to be dominant in Argentina (Quinteros et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In South America, studies from Argentina have shown that bla CTX-M-2 seems to be the most frequent ESBL among Enterobacteriaceae (Quinteros et al 2003). In the Brazilian Southeast Region, bla CTX-M genes were identified in E. coli, Citrobacter amalonaticus, Enterobacter aerogenes and Enterobacter cloacae (Bonnet et al 2000, 2001, Minarini et al 2007), but bla CTX-M was only recently detected in K. pneumoniae (Do Carmo et al 2008, Garcia et al 2008. In this paper we describe the first detection of bla CTX-M genes and class 1 integrons in clinical isolates of K. pneumoniae from the Northeast of Brazil.…”

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blaCTX-M-2 and blaCTX-M-28 extended-spectrum β-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil

Lópes

Veras

Lima

et al. 2010

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

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blaCTX-M-2 and blaCTX-M-28 extended-spectrum β-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil

Lópes

Veras

Lima

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…A new family of ESBLs, CTX-M, has emerged over recent years, especially in E. coli. This family has become one of the most important families of ESBL enzymes in many countries [28][29][30] . CTX-M ß-lactamases are the predominant type of ESBLs in Europe and South America 29,31 , including Brazil 32 .…”

Section: Discussionmentioning

confidence: 99%

Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil

Abreu

Marques

Monteiro-Neto

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. Methods: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the bla TEM and bla CTX-M genes. Results: Among the isolates, 125 were ESBL producers. The bla CTX-M and bla TEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. Conclusions: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae.

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“…Thus, most of the CTX-M producers display levels of resistance to cefotaxime significantly higher than those to ceftazidime. However, the classical phenotype of resistance conferred by CTX-M ␤-lactamases is not universal among all CTX-M producers, since many factors, including production of additional ␤-lactamases (1) or mutations altering the substrate specificity of CTX-M enzymes (8,21,33), can mask their presence. Therefore, the phenotype of resistance to ␤-lactams may suggest the presence of CTX-M enzymes, but this is not a completely reliable approach.…”

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confidence: 99%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

329

181

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A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum ␤-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for CTX-M-type ␤-lactamases, which, as shown by PCR-restriction fragment length polymorphism analysis, were distributed into the two genetic groups of CTX-M-1 (93%)-and CTX-M-2 (7%)-related enzymes. Isolates producing the enzymes of the first group were found in 20 hospitals from geographically distant regions of the country and were characterized by considerable diversity of genetic types, as was demonstrated by enterobacterial repetitive consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15 ␤-lactamases were identified. In contrast, the enzymes of the CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally related E. coli isolates from a single hospital. Notably, the levels of resistance to ceftazidime were remarkably variable among the CTX-M producers. This study provides further evidence of the global dissemination of CTX-M type ESBLs and emphasizes the need for their epidemiological monitoring.During the past decade extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type emerged in many countries of the world. The first organisms producing ␤-lactamases of this type were identified both as single and epidemic clinical isolates in very distant geographic regions (Germany and France and Argentina) in the early 1990s (3, 5, 7). More recently, a rapid increase in the proportion of multiple CTX-M variants to the TEM-and SHV-derived ESBLs has been reported in many hospitals in Spain (9,12,15,35) (14), and Korea (28). Furthermore, CTX-M ␤-lactamases, mainly types CTX-M-2 and CTX-M-3, were found to be widespread or even predominant ESBL types in several countries, including Argentina (31, 34; M. Galas, F. Pasteran, R. Melano, A. Petroni, G. Lopez, A. Corso, A. Rossi, et al., Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-109, 1998), Japan (41), and Poland (1).Currently the CTX-M family includes more than 20 ␤-lactamases, which may be grouped on the basis of sequence similarity into four distinct clusters (subtypes) epitomized by CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 (26, 37; http: //www.lahey.org/studies/webt.htm). Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of ...

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Novel Cefotaximase (CTX-M-16) with Increased Catalytic Efficiency Due to Substitution Asp-240→Gly

Cited by 162 publications

References 38 publications

blaCTX-M-2 and blaCTX-M-28 extended-spectrum β-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil

blaCTX-M-2 and blaCTX-M-28 extended-spectrum β-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil

Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

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