Novel Chitosan Coated Magnetic Nanocarriers for the Targeted Diclofenac Delivery

Agotegaray, Mariela; Palma, Santiago Daniel; Lassalle, Verónica Leticia

doi:10.1166/jnn.2014.8256

Cited by 18 publications

(11 citation statements)

References 10 publications

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“…Results are in agreement with reported data regarding to similar formulations. [12,13] In both cases, the polydispersion indexes registered for all measurements were under 0.5, indicating that N1 and N2 rendered almost monodispersed particles in water.…”

Section: Characterization Of the Obtained Formulationsmentioning

confidence: 97%

“…[12,13] Briefly, 0.3 g of oleic acid (OA) were added, under nitrogen atmosphere and at 70 °C, to 20 mL of an aqueous solution composed by FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 O (Fe 2+ /Fe 3+ ) with an iron molar ratio equal to 0.5. Then, 5 mL of NaOH 5 M were added dropwise.…”

Section: Synthesis Morphological and Magnetic Characterization Of Tmentioning

confidence: 99%

“…[11][12][13] In this work, N1 and N2 were further characterized by Dynamic Light Scattering (DLS), using a Malvern Zetasizer to determine particle hydrodynamic diameters (Dh) and surface charge by measurements of zeta potential (ζ).The measurements were performed employing aqueous dispersions of 0.1 mg MNPS/mL at 25 °C. Data are expressed as mean ± S.D.…”

Section: Synthesis Morphological and Magnetic Characterization Of Tmentioning

confidence: 99%

“…[13] In previous work, we have demonstrated the necessity to better fix chitosan onto the magnetic core to avoid dissagregation. Stability studies performed by UV-Visible spectroscopy, size measurements, [12,13] and TEM analysis have demonstrated instability of chitosan coating.…”

Section: Characterization Of the Obtained Formulationsmentioning

confidence: 99%

“…polydispersity, surface charge, and ability to bind to a model drug (Diclofenac). [12,13] Then this contribution proposes the following step in view of the practical implementation of these nanosystems as targetable drug delivery systems related to pre-clinical studies at vascular and biodistribution levels.…”

Section: Introductionmentioning

confidence: 99%

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Influence of chitosan coating on magnetic nanoparticles in endothelial cells and acute tissue biodistribution

Agotegaray

Campelo

Zysler

et al. 2016

Journal of Biomaterials Science, Polymer Edition

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Chitosan coating on magnetic nanoparticles (MNPs) was studied on biological systems as a first step toward the application in the biomedical field as drug-targeted nanosystems. Composition of MNPs consists of magnetite functionalized with oleic acid and coated with the biopolymer chitosan or glutaraldehyde-cross-linked chitosan. The influence of the biopolymeric coating has been evaluated by in vitro and in vivo assays on the effects of these MNPs on rat aortic endothelial cells (ECs) viability and on the random tissue distribution in mice. Results were correlated with the physicochemical properties of the nanoparticles. Nitric oxide (NO) production by ECs was determined, considering that endothelial NO represents one of the major markers of ECs function. Cell viability was studied by MTT assay. Different doses of the MNPs (1, 10 and 100 μg/mL) were assayed, revealing that MNPs coated with non-cross-linked chitosan for 6 and 24 h did not affect neither NO production nor cell viability. However, a significant decrease in cell viability was observed after 36 h treatment with the highest dose of this nanocarrier. It was also revealed that the presence and dose of glutaraldehyde in the MNPs structureimpact on the cytotoxicity. The study of the acute tissue distribution was performed acutely in mice after 24 h of an intraperitoneal injection of the MNPs and sub acutely, after 28 days of weekly administration. Both formulations greatly avoided the initial clearance by the reticuloendothelial system (RES) in liver. Biological properties found for N1 and N2 in the performed assays reveal that chitosan coating improves biocompatibility of MNPs turning these magnetic nanosystems as promising devices for targeted drug delivery.

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Section: Characterization Of the Obtained Formulationsmentioning

confidence: 97%

Section: Synthesis Morphological and Magnetic Characterization Of Tmentioning

confidence: 99%

Section: Synthesis Morphological and Magnetic Characterization Of Tmentioning

confidence: 99%

Section: Characterization Of the Obtained Formulationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of chitosan coating on magnetic nanoparticles in endothelial cells and acute tissue biodistribution

Agotegaray

Campelo

Zysler

et al. 2016

Journal of Biomaterials Science, Polymer Edition

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Development of Magnetic Nanosystems with Potential for the Treatment of Inner Ear Pathologies

Julia Martín,

Schneider,

Paolillo

et al. 2024

ChemMedChem

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Hearing loss (HL) affects more than 5% of the global population, with projections indicating an impact of up to 50% on young individuals in the next years. HL treatments remain limited due to the inner ear's hermeticism. HL often involves inflammatory processes, underscoring the need for enhanced delivery of antiinflammatory agents to the inner ear. Our research focuses on the development of a directed therapy based on magnetic nanoparticles (MNPs). We previously synthesized biocompatible folic acid‐coated iron oxide‐core nanoparticles (MNPs@FA) as potential carriers for the anti‐inflammatory Diclofenac (Dfc). This study aims to incorporate Dfc onto MNPs@FA to facilitate targeted drug delivery to the inner ear. Through optimizing the loading procedure, we achieved optimal loading capacity. Dfc release was studied in the simulated target fluid and the administration vehicle. Complete characterization is also shown. In vitro biocompatibility testing ensured the biosafety of the resulting formulation. Subsequent ex vivo targeting assays on murine cochleae validated the nanosystems' ability to penetrate the round window membrane, one of the main HL therapy barriers. These findings serve as validation before continuing to more complex in vivo studies. Together, the data here presented represent an advancement in addressing unmet medical needs in HL therapy.

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Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

et al. 2014

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The effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.

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Novel Chitosan Coated Magnetic Nanocarriers for the Targeted Diclofenac Delivery

Cited by 18 publications

References 10 publications

Influence of chitosan coating on magnetic nanoparticles in endothelial cells and acute tissue biodistribution

Influence of chitosan coating on magnetic nanoparticles in endothelial cells and acute tissue biodistribution

Development of Magnetic Nanosystems with Potential for the Treatment of Inner Ear Pathologies

Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

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