Novel chitosan hydrogels reinforced by silver nanoparticles with ultrahigh mechanical and high antibacterial properties for accelerating wound healing

Xie, Yajuan; Liao, Xi; Zhang, Jingxiang; Yang, Feiwen; Fan, Zengjie

doi:10.1016/j.ijbiomac.2018.07.060

Cited by 165 publications

(92 citation statements)

References 54 publications

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“…Xie et al, found that entrapment of silver nanoparticles into chitosan hydrogels could increase its antibacterial activity. In addition to wound healing properties of hydrogel, this study also showed that the hydrogel significantly improved the re-epithelialization and collagen deposition 41 . Another study by Kumar et al on chitosan hydrogel/nano zinc oxide composite bandages (CZBs) indicated that combination of zinc oxide nanoparticles with chitosan hydrogel improved antibacterial activity and wound healing.…”

Section: Discussionmentioning

confidence: 63%

Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

Golmohammadi

Peerayeh

Moghadam

et al. 2020

Sci Rep

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the current study aimed to formulate Selenium-chitosan-Mupirocin (M-Senps-ccH) complex. the nanohybrid system was prepared using chitosan-cetyltrimethylammonium bromide (ctAB)based hydrogel (ccH) that entrapped mupirocin (M) and selenium nanoparticles (Senps). the in vitro studies were performed by evaluation of the antibacterial activity and toxicity on L929 mouse fibroblast cell line. The in vivo study was conducted on rat diabetic wound infection model that was infected by mupirocin-methicillin-resistant Staphylococcus aureus (MMRSA). the wounds were treated by M-SeNPs-CCH nanohybrid system with concentrations of M; 20 mg/ml, CCH; 2 mg/ml and SeNPs; 512 μg/ml in two times/day for 21 days. The therapeutic effect of this nanohybrid system was evaluated by monitoring wound contraction and histopathological changes. evaluation of the average wound healing time showed a significant difference between the treatment and control groups (P≤0.05). The histopathological study indicated that the amount of wound healing was considerable in M-Senps-ccH nanohybrid system groups compared to the control and M groups. the M-Senps-CCH nanohybrid system formulated in this study was able to reduce 3-fold MIC of mupirocin with synergistic antibacterial activity as well as to play a significant role in wound contraction, angiogenesis, fibroblastosis, collagenesis, proliferation of hair follicle, and epidermis growth compared to the control group (P ≤ 0.05). This research suggests that this nanohybrid system might be a development for the treatment of diabetic wound infection at mild stage. Management of the infectious diabetic foot ulcer remains a global health issue 1,2. According to the World Health Organization (WHO), about 422 million people worldwide suffer from diabetes 2. About 25% of diabetic patients experience diabetic foot wounds in their lifetime 3,4 , half of them suffer infection 3. Diabetic wounds are classified into three groups, i.e. mild, moderate and severe 3. To avoid amputation and reduce health care costs, it is essential to control the infection 5. Gram-positive bacteria such as Staphylococcus aureus and beta-hemolytic streptococci are common infectious agents in the mild stage of diabetic foot wounds 3,5. Regarding the increasing incidence of antibiotic-resistant strains such as methicillin-resistant S. aureus (MRSA) 6,7 , the treatment of infection caused by this organism has

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Section: Discussionmentioning

confidence: 63%

Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

Golmohammadi

Peerayeh

Moghadam

et al. 2020

Sci Rep

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“…Such nanocomposites demonstrated minimum bactericidal concentration values of 39.1 and 312.5 µg/ml for E. coli and S. aureus, respectively, and did not exhibit cytotoxicity to L-929 fibroblasts [24]. Chitosan hydrogels, reinforced with silver nanoparticles, revealed ultra-high mechanical and high antibacterial properties against E. coli and S. aureus and were used to accelerate wound healing [25].…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Deposition and Characteristics of Chitosan–Nanosilver Composite Coatings on a Nanotubular TiO2 Layer

et al. 2020

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The surface treatment of titanium implants has been applied mainly to increase surface bioactivity and, more recently, to introduce antibacterial properties. To this end, composite coatings have been investigated, particularly those based on hydroxyapatite. The present research was aimed at the development of another coating type, chitosan–nanosilver, deposited on a Ti13Zr13Nb alloy. The research comprised characterization of the coating’s microstructure and morphology, time-dependent nanosilver dissolution in simulated body fluid, and investigation of the nanomechanical properties of surface coatings composed of chitosan and nanosilver, with or without a surface-active substance, deposited at different voltages for 1 min on a nanotubular TiO2 layer. The microstructure, morphology, topography, and phase composition were examined, and the silver dissolution rate in simulated body fluid, nanoscale mechanical properties, and water contact angle were measured. The voltage value significantly influenced surface roughness. All specimens possessed high biocompatibility. The highest and best adhesion of the coatings was observed in the absence of a surface-active substance. Silver dissolution caused the appearance of silver ions in solution at levels effective against bacteria and below the upper safe limit value.

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“…Similar to the second example, mesh dependence is investigated by comparing two cases (740 elements and 4230 H(d,t) is similar for both meshes. Results in figure 14 show a healing process which is comparable to in vivo wound healing of rats reported in [43], in terms of both duration and size of the damage area, although the gain parameter and the internal length scales are set arbitrarily. The effect of internal length scales in the healing process is investigated by setting different c d values (c d is a parameter related to the average length of the microstructural components in the soft tissues).…”

Section: Simulation Of Wound Healingmentioning

confidence: 61%

“…The damage threshold can be determined by in vitro experimental measurements for different kinds of tissues, and the effects of inter-individual variability can be predicted by sensitivity analyses. For instance, the wound-healing experiments in rats reported by [43] could provide essential data about the evolution of the area of damage to identify the healing parameters. The choice of the PK-2 principal stress direction in computing the growth directions is another limitation, as PK- 2 stress has no physical meaning.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional numerical simulation of soft-tissue wound healing using constrained-mixture anisotropic hyperelasticity and gradient-enhanced damage mechanics

Zuo

Avril

Yang

et al. 2020

J. R. Soc. Interface.

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Healing of soft biological tissues is the process of self-recovery or self-repair after injury or damage to the extracellular matrix (ECM). In this work, we assume that healing is a stress-driven process, which works at recovering a homeostatic stress metric in the tissue by replacing the damaged ECM with a new undamaged one. For that, a gradient-enhanced continuum healing model is developed for three-dimensional anisotropic tissues using the modified anisotropic Holzapfel–Gasser–Ogden constitutive model. An adaptive stress-driven approach is proposed for the deposition of new collagen fibres during healing with orientations assigned depending on the principal stress direction. The intrinsic length scales of soft tissues are considered through the gradient-enhanced term, and growth and remodelling are simulated by a constrained-mixture model with temporal homogenization. The proposed model is implemented in the finite-element package Abaqus by means of a user subroutine UEL. Three numerical examples have been achieved to illustrate the performance of the proposed model in simulating the healing process with various damage situations, converging towards stress homeostasis. The orientations of newly deposited collagen fibres and the sensitivity to intrinsic length scales are studied through these examples, showing that both have a significant impact on temporal evolutions of the stress distribution and on the size of the damage region. Applications of the approach to carry out in silico experiments of wound healing are promising and show good agreement with existing experiment results.

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Novel chitosan hydrogels reinforced by silver nanoparticles with ultrahigh mechanical and high antibacterial properties for accelerating wound healing

Cited by 165 publications

References 54 publications

Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

Electrophoretic Deposition and Characteristics of Chitosan–Nanosilver Composite Coatings on a Nanotubular TiO2 Layer

Three-dimensional numerical simulation of soft-tissue wound healing using constrained-mixture anisotropic hyperelasticity and gradient-enhanced damage mechanics

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