Novel circovirus in European catfish (Silurus glanis)

Lőrincz, Márta; Dán, Ádám; López-Pavía, María; Csaba, G.; Tóth, Ágnes; Székely, Csaba; Cságola, Attila; Tuboly, Tamás

doi:10.1007/s00705-012-1291-1

Cited by 52 publications

(55 citation statements)

References 10 publications

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“…In fact, until 2010, pigs were the only mammals known to be affected by circoviruses and most of the diversity for this group of viruses was reported from avian species (Table 1). However, studies employing viral metagenomic-based strategies and degenerate PCR for circoviruses in unconventional hosts have since identified the presence of circovirus genomes in freshwater fish [45,46] and various mammals, including bats [34-36, 42, 91], chimpanzees [34], dogs [37], humans [34] and minks [41]. Although a definitive host has not been confirmed for some of these newly-detected circoviruses (e.g., bat-associated circoviruses), phylogenetic analyses indicate that circovirus genomes detected in mammals, in general, are more closely-related to each other than to avian circoviruses (Fig.…”

Section: Genus Circovirusmentioning

confidence: 99%

Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus

et al. 2017

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Section: Genus Circovirusmentioning

confidence: 99%

Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus

et al. 2017

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“…Circoviruses have an ambisense genome organization containing two major open reading frames (ORFs), rep and cap, on opposite strands of the double-stranded DNA replicative intermediate. While the initial descriptions of circoviruses were mainly from avian species, numerous members of the family Circoviridae have been characterized in fish, insects, and mammals (2)(3)(4). Two species of circovirus are known to infect pigs.…”

mentioning

confidence: 99%

A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure

Palinski

Piñeyro

Shang

et al. 2017

J Virol

480

744

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Porcine circovirus-associated disease (PCVAD) is clinically manifested by postweaning multisystemic wasting syndrome (PMWS), respiratory and enteric disease, reproductive failure, and porcine dermatitis and nephropathy syndrome (PDNS). Porcine circovirus 2 (PCV2) is an essential component of PCVAD, although an etiologic role in PDNS is not well established. Here, a novel circovirus, designated porcine circovirus 3 (PCV3), was identified in sows that died acutely with PDNS-like clinical signs. The capsid and replicase proteins of PCV3 are only 37% and 55% identical to PCV2 and bat circoviruses, respectively. Aborted fetuses from sows with PDNS contained high levels of PCV3 (7.57 ϫ 10 7 genome copies/ml), and no other viruses were detected by PCR and metagenomic sequencing. Immunohistochemistry (IHC) analysis of sow tissue samples identified PCV3 antigen in skin, kidney, lung, and lymph node samples localized in typical PDNS lesions, including necrotizing vasculitis, glomerulonephritis, granulomatous lymphadenitis, and bronchointerstitial pneumonia. Further study of archived PDNS tissue samples that were negative for PCV2 by IHC analysis identified 45 of 48 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testing positive for PCV3 by IHC analysis. Analysis by qPCR of 271 porcine respiratory disease diagnostic submission samples identified 34 PCV3-positive cases (12.5%), and enzyme-linked immunosorbent assay detection of anti-PCV3 capsid antibodies in serum samples found that 46 (55%) of 83 samples tested were positive. These results suggest that PCV3 commonly circulates within U.S. swine and may play an etiologic role in reproductive failure and PDNS. Because of the high economic impact of PCV2, this novel circovirus warrants further studies to elucidate its significance and role in PCVAD.IMPORTANCE While porcine circovirus 2 (PCV2) was first identified in sporadic cases of postweaning multisystemic wasting syndrome in Canada in the early 1990s, an epidemic of severe systemic disease due to PCV2 spread worldwide in the ensuing decade. Despite being effectively controlled by commercial vaccines, PCV2 remains one of the most economically significant viruses of swine. Here, a novel porcine circovirus (PCV3) that is distantly related to known circoviruses was identified in sows with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive failure. PCV2, which has previously been associated with these clinical presentations, was not identified. High levels of PCV3 nucleic acid were observed in aborted fetuses by quantitative PCR, and PCV3 antigen was localized in histologic lesions typical of PDNS in sows by immunohistochemistry (IHC) analysis. PCV3 was also identi- Citation Palinski R, Piñeyro P, Shang P, Yuan F, Guo R, Fang Y, Byers E, Hause BM. 2017. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J Virol 91:e01879-16.

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“…More specifically, the putative bream CV resembled most the two barbel circoviruses (BaCV1 and 2) reported from Hungary (Lőrincz et al, 2011), whereas the sequence derived from the common toad seemed to be monophyletic with the European catfish circovirus (CfCV) also described by Hungarian researchers more recently (Lőrincz et al, 2012). Interestingly, the putative CV supposedly present in the dead and live specimens of captive green tree frogs clustered closest to the branch of the bird CVs, although the node support (75) calculated by bootstrap analysis was not very strong.…”

Section: Acta Veterinaria Hungarica 62 2014mentioning

confidence: 99%

“…Among these, a common bream sample contained a rep sequence most closely related to that of the barbel CVs (Lőrincz et al, 2011), whereas in the samples from three other fish species, namely a roach (Rutilus rutilus), an asp (Aspius aspius) and a round goby (Neogobius melanostomus), further three novel rep sequences were detected. Among the amphibian samples, a putative new CV, resembling most the European catfish CV, published recently (Lőrincz et al, 2012), was detected in the carcass of a common toad (Bufo bufo). In case of the green tree frog (Litoria caerulea) samples, we detected identical nucleotide sequences which imply the possible presence of the same, novel CV in a dead and a live animal.…”

Section: Pcr and Sequencingmentioning

confidence: 99%

First detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes

Tarján

Pénzes

Tóth

et al. 2014

Acta Veterinaria Hungarica

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The negative samples of a collection, established originally for seeking new adeno-and herpesviruses in lower vertebrates, were screened for the presence of circoviruses by a consensus nested PCR targeting the gene coding for the replication-associated protein. Six fish samples representing five species, namely asp (Aspius aspius), roach (Rutilus rutilus), common bream (Abramis brama), round goby (Neogobius melanostomus) and monkey goby (Neogobius fluviatilis), as well as three frog samples were found positive for circoviral DNA. Sequence analysis of the amplicons indicated the presence of three novel putative circo-like viruses and a circovirus in Hungarian fishes and one novel circovirus in a common toad (Bufo bufo), and another one in a dead and an alive specimen of green tree frog (Litoria caerulea), respectively. In phylogeny reconstruction, the putative bream circovirus clustered together with circoviruses discovered in other cyprinid fishes recently. Three other piscine circoviral sequences appeared closest to sequences derived from different environmental samples. Surprisingly, the nucleotide sequence derived from two fish samples (a bream and a monkey goby) proved to be from porcine circovirus 2 (PCV2), almost identical to a sequence detected in Sweden previously. This is the first report on the detection of PCV2 in fish and circoviral DNA in amphibian hosts.

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Novel circovirus in European catfish (Silurus glanis)

Cited by 52 publications

References 10 publications

Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus

Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus

A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure

First detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes

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