Novel cis-acting elements in petunia Cab gene promoters

Abstract

In order to identify specific cis-acting elements which regulate the expression of the divergent Cab22R and Cab22L genes of Petunia, we conducted systematic mutational studies of the 1 kb intergenic promoter region. Sequence analysis revealed three GATA box sequence repeats positioned between the TATA and CAAT box elements. These GATA elements are conserved in corresponding promoter regions of all LHCII Type I Cab genes in Petunia and other dicotyledonous plants we have examined. Site-specific mutations in the CAAT box and the GATA box elements of the Cab22R promoter resulted in 8-fold and 5-fold reductions in Cab22R transcript levels respectively. A deletion of 52 bp, adjacent and upstream from the CAAT box (-92 to -145) in the Cab22R promoter reduced transcript levels 20-fold. This deletion contains a region of 13 bp which is conserved between many Petunia Cab genes. These results indicate that the quantitative expression of the Cab22 promoters is regulated by multiple cis-acting elements including CAAT and GATA box elements as well as sequences located between -92 and -145. The deletion of the region between -92 and -145 is partially compensated by homologous sequences present in the adjacent divergent promoter Cab22L.

Ancestral Multipartite Units in Light-Responsive Plant Promoters Have Structural Features Correlating with Specific Phototransduction Pathways

“…Since a cab gene (P. thunberghii Cab 6) containing only modules 1 and 4 seems to be nonphotoregulated (Kojima et al, 1992(Kojima et al, , 1994, the minimal D N A modular array, which is consistently associated with light-responsive regions, is the one constituted by modules 1 , 2, and 4. Individual conserved DNA modules are: 1 to 3, GATA motifs (Gidoni et al, 1989); 4, CCAAT box; 5 , motif related to the ABF-2-binding site (Argüello et al, 1992) and contained within the region bound by CA-1, CUF2, and CUF3 factors (Sun et al, 1993;Carré and Kay, 1995); 6, CUF-1 binding-site (Anderson et al, 1994).…”

“…The latter display distinct "broadened" consensus in dicotyledons, which are RGATGAGATAAGAT (20 genes from species belonging to 10 genera and five families compared; not shown) and TGGTGNNYAAYGATAAGG (derived from 11 genes from plants belonging to four dicotyledonous families) for rbcS-CMA5 and rbcS-CMA4 IB modules, respectively. Cab-CMA1 also includes an IB element, which is part of a conserved array of three GATA motifs found in many cab gene promoters (Gidoni et al, 1989) and in which the consensus in angiosperms is GA-TANNGATAN(6-8)GATAAGR (Fig. 2).…”

“…For instance, several plant mutants exist in which PhANGs are expressed in the dark at levels that are similar to those in the light (reviewed by McNellis and Deng, 1995), and promoter regions from some of those genes have been shown to contain cis-acting elements that function as silencers in the absence of light (Kuhlemeier et al, 1987(Kuhlemeier et al, , 1989Stockhaus et al, 1989). On the other hand, mutational studies from PhANG promoters have established a positive role in transcription for a variety of IB-related motifs, including some unequivocally defined as phytochrome-responsive elements (Gidoni et al, 1989;Donald and Cashmore, 1990;Schafner and Sheen, 1991;Kehoe et al, 1994;Anderson and Kay, 1995;Argüello-Astorga and Herrera-Estrella, 1995;I. Meier and W. Gruissem, unpublished data).…”

Isolation of an ion channel gene from Arabidopsis thaliana using the H5 signature sequence from voltage‐dependent K+ channels

“…The second motif is the light-responsive element GATA (-384 to -387). This sequence regulates transcription of both rhcS (small subunit of ribulose-1,5-bisphosphate carboxylase) and Cub (chlorophyll a/b binding protein) and is present in the promoter region of many genes essential for photosynthesis [23]. There are nine introns in the AKT3 genomic sequence, ranging in size from 79 to 256 bp (Table I).…”

Multiple transcripts of a gene for a leucine-rich repeat receptor kinase from morning glory (Ipomoea nil) originate from different TATA boxes in a tissue-specific manner

“…The TATA element beginning at nt 524 deviates from the consensus TATA box defined by Bucher (1990), but is similar to an artificial TATA box construct that results in low RNA polymerase II activity in vitro in the absence of other cis -acting elements (Wang et al 1996) and is also similar to the TATA box associated with the petunia cab22R gene (Gidoni et al 1989). The low activity of these TATA boxes suggests a plausible reason why only a small number of transcripts initiate downstream of TATA 524 at nt 561.…”