Novel Clinical Trial for Treatment of Infected Subcutaneous Fistula in Female Griffon Dog using Gold Nano-Rod Assisted Near-Infrared Plasmonic Photothermal Therapy

Abstract

Abstract:Raises in the occurrence of antibiotic resistant bacterial infections need novel methods for control. It is currently clear that a nanotechnology-driven method using nanoparticles to target and terminate pathogenic bacteria can be positively applied. Plasmonic photothermal therapy (PPTT) is a hopeful minimally-invasive antibacterial therapy as well as oncological treatment strategy where plasmonic nanoparticles are used to transform near infrared light to localized heat to cause cell death, mainly through apoptosis and/ or necrosis. The objective of this work was to detect the clinical bactericidal activities of gold nanorods (AunRs) using plasmonic photothermal therapy (PPTT) against pathogenic extended spectrum Beta Lactamase (ESβL) Klebsiella pneumoniae as well as apoptotic actions against pyogenic membrane of chronic subcutaneous fistula had no tendency to heal in clinically affected eight years old Griffon female dog. The fistula was infiltrated along its pyogenic wall and intraluminal in multiple spots with AuNR at 7.5 nM concentration and then exposed to an 808 nm diode laser with power of 5.8 W/cm2 and spot size about 5.6 mm2. The PPTT session was repeated every two weeks for four successive sessions. The evaluations of the curative response were done through clinical, bacteriological and histopathoilogical repetitive examinations. Clinically, the purulent discharge decreased in the quantity until it disappeared at the third PPTT session and complete regression of the fistula occurred after the fourth PPTT application. Bacteriologically, Klebsiella pneumoniae isolate showed dramatic reduction in colony forming unit (CFU) count after first session and was completely absent at the fourth PPTT session. Regarding histopathology of the tissue specimens from infected fistula when the case was admitted to the clinic, there was massive liquefied necrotic area infiltrated mainly by neutrophils represented as grade +3 and after first session of PPTT, there was circumscribe necrotic area infiltrated by few numbers of neutrophils and represented as grade +1. After third session of PPTT, complete absence of necrotic tissues was recorded and new fibrous connective tissue formation was observed. Altogether, these outcomes powerfully recommend that AunRs could be a motivating choice to control antibiotic resistant bacterial infections as well as pyogenic conditions which have no tendency to heal 151

Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

“…Wound infections with multidrug-resistant strains of pathogenic microorganisms are significant global health problems. The economic loads and morbidity and mortality rates are immense and have attracted researchers to find novel methods for infection management 1,2…”