Novel co-enrichment method for isolation of magnetotactic bacteria

Abstract

A novel co-enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux. We successfully enriched and isolated both North seeking as well as South seeking magnetotactic bacteria from Lonar Lake (Buldhana), Moti Lake (Jalna), Ghanewadi Lake (Jalna), Ganesh Lake (Miraj), Rankala Lake (Kolhapur), and industrial metal-contaminated glaying soils (Jalna) and a soil (Karad), (MS, India) exposed to high-voltage electric current. The hanging drop preparations and growth under magnetic stress on low-agar media allowed conformation of magnetotactic behavior of the isolates. Both Gram positive and Gram negative MTB were isolated with diverse morphologies. South seeking population was more predominant. The soil inhabitants showed little dwelling property which was more prominent in case of aquatic inhabitants. The use of in situ pH and salt concentrations during enrichment and isolation found suited. The simultaneous growth of whole consortium in the system ensured the in situ simulation of microenvironment needful for proper growth of fastidious MTB.

Alleviation of drought stress in pulse crops with ACC deaminase producing rhizobacteria isolated from acidic soil of Northeast India

“…The members of the consortium were also efficient producers of bacterial siderophores. The siderophores play a significant role in the biological availability of iron to plant roots 55 . Low molecular mass siderophores can bind to most of the iron available in the rhizosphere with very high avidity, which inhibits the proliferation of fungal pathogens in host plant roots due to lack of available iron 56 , 57 .…”

Yield cultivation of magnetotactic bacteria and magnetosomes: A review

“…Table depicts the real situation concerning research development regarding MTB and its yield cultivation since its discovery. Recently, Sorty and Shaikh designed a novel co‐enrichment technique for MTB. This technique was less time consuming, less laborious/tedious, does not require any sophisticated instruments and secondary enrichment.…”

“…MTB are the groups of prokaryotes that can synthesis intracellular bio‐magnetite minerals or MNPs known as magnetosomes (MS) by a strictly controlled biomineralization process . Due to the presence of these MS, MTB have the ability to move along the applied external magnetic field .…”

Diversity and ecology of and biomineralization by magnetotactic bacteria

“…Nash () identified a thermophilic MTB from Little Hot Creek (45–55°C) and four from Mono Lake (pH of 9.8 and salinity 80.8 gL −1 ). This was the first indication that some MTB thrive in extreme environments including hot springs (Lefèvre et al ., ), saline‐alkaline lakes (Lefèvre et al ., ; Sorty and Shaikh, ) and deep‐sea environments (Dong et al ., ). We recently found many types of MTB in several saline‐alkaline lakes in Inner Mongolia, China, with pH ranges between 8.9 and 9.8 and salinity values between 5.5 and 58.0 ppt (Fig.…”