Novel cold-adapted raw-starch digesting α-amylases from Eisenia fetida: Gene cloning, expression, and characterization

Abstract

Highlights There have been few reports about gene cloning and expression of α-amylases from E. fetida . Ef-Amy I and II were shown to 89% identity of amino acid sequences. The catalytically important residues of α-amylase of GH family 13 were conserved in Ef-amy I and II. The substrate specificities of rEf-Amy I and II were dissimilar. It found that rEf-Amy I and II could be possible use for simultaneous saccharification and fermentation process.