Novel combination of serum microRNA for detecting breast cancer in the early stage

Shimomura, Akihiko; Shiino, Sho; Kawauchi, Junpei; Takizawa, Satoko; Sanada, Hiromi; Matsuzaki, Juntaro; Ono, Makiko; Takeshita, Fumitaka; Niida, Shumpei; Shimizu, Chikako; Fujiwara, Yasuhiro; Kinoshita, Takayuki; Tamura, Kenji; Ochiya, Takahiro

doi:10.1111/cas.12880

Cited by 292 publications

(280 citation statements)

References 24 publications

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“…Particularly, many miRNAs including miR-1307 have been linked to chemo-resistance to many kinds of chemotherapy drugs in ovarian cancer [2, 4, 7–9, 13–16]. Recently, miR-1307 was found to be up-regulated in a variety of cancers, such as breast cancer, liver cancer, colorectal tumor and ovarian cancer [7, 21–24]. For example, miR-1307-3p was found to be useful for detecting breast cancer in the early stage [21].…”

Section: Discussionmentioning

confidence: 99%

MiR-1307 promotes ovarian cancer cell chemoresistance by targeting the ING5 expression

et al. 2017

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BackgroundWe aimed to investigate the function of miR-1307 in chemoresistance and to explore its chemoresistance mechanism in ovarian cancer.MethodsIC50 determination was used to test the chemoresistance profling in ovarian cancer cells. QRT-PCR or western blot was used to validate the expression level of miR-1307 and candidate gene or protein. Colony formation assay and FITC-labeled enhanced Annexin V immunofluorescence were used to compare cell proliferation and apoptosis ability, respectively. The potential target gene and its biological function of miRNA-1307 were also analyzed. Bioinformatics and Luciferase Reporter Gene Assay were conducted to validate the regulation of miRNA-1307 on the ING5 expression. Xenografts assay was used to demonstrate the inhibiting effect of miR-1307 ASO and Taxol therapy against ovarian cancer in vivo.ResultsMiR-1307 was over-expressed in chemoresistant ovarian cancer cell line A2780/Taxol, and over-expression or loss of miR-1307 promoted or inhabited chemoresistance. And we also found that the over-expression of miR-1307 promoted proliferation and inhibited apoptosis in ovarian cancer cells. Besides, we demonstrated that ING5 was a direct target of miR-1307 and miR-1307 down-regulated the ING5 expression in ovarian cancer cells. Additionally, we showed that ING5 inhibited cell proliferation, promoted cell apoptosis and inhabited chemoresistance reversely. Furthermore, the up-regulated ability of cell apoptosis and down-regulated ability of chemoresistance following the loss of miR-1307 was reversed by adding ING5 siRNA in vitro. Finally, we proved the inhibiting effect of miR-1307 ASO and Taxol therapy by increasing the ING5 expression against ovarian cancer through xenografts assay in vivo.ConclusionOur results suggested that miR-1307 could promote ovarian cancer chemoresistance by targeting the ING5 expression and miR-1307 might serve as a therapeutic target for ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

MiR-1307 promotes ovarian cancer cell chemoresistance by targeting the ING5 expression

et al. 2017

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“…This miRNA has also an impact on metastasis-free survival and event-free survival rates, independently of ER status [154]. Hsa-miR-1246 was included in a 5-miRNA signature with good diagnostic features, able to discriminate between healthy and early stage BC samples [155]. …”

Section: Discussionmentioning

confidence: 99%

How interacting pathways are regulated by miRNAs in breast cancer subtypes

Cava

Colaprico²,

Bertoli

et al. 2016

BMC Bioinformatics

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BackgroundAn important challenge in cancer biology is to understand the complex aspects of the disease. It is increasingly evident that genes are not isolated from each other and the comprehension of how different genes are related to each other could explain biological mechanisms causing diseases. Biological pathways are important tools to reveal gene interaction and reduce the large number of genes to be studied by partitioning it into smaller paths. Furthermore, recent scientific evidence has proven that a combination of pathways, instead than a single element of the pathway or a single pathway, could be responsible for pathological changes in a cell.ResultsIn this paper we develop a new method that can reveal miRNAs able to regulate, in a coordinated way, networks of gene pathways. We applied the method to subtypes of breast cancer. The basic idea is the identification of pathways significantly enriched with differentially expressed genes among the different breast cancer subtypes and normal tissue. Looking at the pairs of pathways that were found to be functionally related, we created a network of dependent pathways and we focused on identifying miRNAs that could act as miRNA drivers in a coordinated regulation process.ConclusionsOur approach enables miRNAs identification that could have an important role in the development of breast cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12859-016-1196-1) contains supplementary material, which is available to authorized users.

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“…Several studies have shown that exosomal miRNAs can be detected in a variety of body fluids, from blood to breast milk, and that their levels are correlated with patient status [4,5,[60][61][62][63][64][65][66][67][68][69][70][71][72][73] (Table 1). On January 21, 2016, cancer diagnosis using exosomes from blood became commercially available in the US (http://www.exosomedx.com) [6].…”

Section: Diagnosismentioning

confidence: 99%

“…Like cellular miRNA expression profiles, the expression profiles of cell-free and circulating miRNAs are also correlated with the pathological condition of cancer patients [4,60]. Therefore, a considerable amount of research has been expended in trying to develop novel methods for the detection and characterization of microvesicles in the blood of patients classified according to clinical stage [74][75][76].…”

Section: Diagnosismentioning

confidence: 99%

The role of extracellular vesicle microRNAs in cancer biology

Takahashi¹,

Prieto‐Vila²,

Hironaka³

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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microRNAs (miRNAs) constitute a large family of small, approximately 20-22 nucleotide non-coding RNAs that regulate the expression of target genes, mainly at the post-transcriptional level. Multiple studies report that miRNAs are involved in homeostatic maintenance and that aberrant expression of miRNAs is often observed in various types of diseases, including cancer. In cancer biology, miRNAs exert functional roles in tumor initiation, drug resistance, and metastasis. miRNAs are also secreted through small vesicles called exosomes, which are endosome-derived vesicles derived from various cell types including immune and tumor cells. In addition to cellular miRNAs (ce-miRNAs), secreted miRNAs (se-miRNAs) play important roles in cancer development and metastasis. Therefore, se-miRNAs in body fluids have been investigated as a promising biomarkers and therapeutic targets for cancer treatment. In this review, we summarize the current knowledge of miRNA functions in cancer development and discuss the potential clinical applications of se-miRNAs, e.g. as diagnostic markers and therapeutic targets.

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Novel combination of serum microRNA for detecting breast cancer in the early stage

Cited by 292 publications

References 24 publications

MiR-1307 promotes ovarian cancer cell chemoresistance by targeting the ING5 expression

MiR-1307 promotes ovarian cancer cell chemoresistance by targeting the ING5 expression

How interacting pathways are regulated by miRNAs in breast cancer subtypes

The role of extracellular vesicle microRNAs in cancer biology

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